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The map label for this gene is ltrBE1 [H]

Identifier: 116516207

GI number: 116516207

Start: 946388

End: 947005

Strand: Direct

Name: ltrBE1 [H]

Synonym: SPD_0936

Alternate gene names: 116516207

Gene position: 946388-947005 (Clockwise)

Preceding gene: 116517032

Following gene: 116515758

Centisome position: 46.25

GC content: 34.14

Gene sequence:

>618_bases
ATGGTAGTTACTAAACACTTTGCGACGCACGGAAAAAAATATCGTAGGCGTTTGATTAAATATATCCTCAATCCAGATAA
AACAGATAATCTAAAATTAGTATCTGATTTTGGCATGAGTAATTACTTAGATTTTCCTAGCCATACAGAAATGGTAGAAA
TGTACAATGTTAACTTTACTAATAACGACAAGTTGTACGAGTCCAGAAATGACCGACAAGAAAAACACCAACAAACTATT
CATGCCCATCATCTCATCCAATCATTTTCTCCTGAAGATAATCTGACACCTGAAGAAATTAACCGCATTGGTTATGAGAC
CATGATGGAATTGACAGGAGGTCGTTTTAAGTTTATCGTAGCAACTCACACAGACAAAGATCATGTTCATAATCACATCC
TAATCAACGCTATTGACCGCAATTCAGATAAAAAGTTTATATGGAACTATGCCTTGGAACGAAACCTCCGTATGATTTCA
GACCGTATTTCTAAAATGGCTGGTGCAAAAATTATTGAGAAACGTTATTCTTATCGAGGTTATAAAAAATATAGGGAATC
TAGTCATAAGTTTGAATTGAAACAACGTCTTTATTTTTTGATGCAGCAATCTAAATAG

Upstream 100 bases:

>100_bases
TCTCAGGACCAATTACAAGAATTGAGTAAAGGAGTCGGAGAGTTAATTAAGGAAGTGGACAAAGAATTTCAAGTGTAGGT
GAAAAGACTGAAGGAGTTTC

Downstream 100 bases:

>100_bases
TTTGATGATTTTTTAGAGAAAGCAAAGCAGTTGCATGTTCAGATTGATTTTAGTCAAAAGCATAGTCGATTCATGATGAC
GGATCGAGCAATGACAAAAC

Product: Tn5252, relaxase

Products: NA

Alternate protein names: Conjugative nickase [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLDFPSHTEMVEMYNVNFTNNDKLYESRNDRQEKHQQTI
HAHHLIQSFSPEDNLTPEEINRIGYETMMELTGGRFKFIVATHTDKDHVHNHILINAIDRNSDKKFIWNYALERNLRMIS
DRISKMAGAKIIEKRYSYRGYKKYRESSHKFELKQRLYFLMQQSK

Sequences:

>Translated_205_residues
MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLDFPSHTEMVEMYNVNFTNNDKLYESRNDRQEKHQQTI
HAHHLIQSFSPEDNLTPEEINRIGYETMMELTGGRFKFIVATHTDKDHVHNHILINAIDRNSDKKFIWNYALERNLRMIS
DRISKMAGAKIIEKRYSYRGYKKYRESSHKFELKQRLYFLMQQSK
>Mature_205_residues
MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLDFPSHTEMVEMYNVNFTNNDKLYESRNDRQEKHQQTI
HAHHLIQSFSPEDNLTPEEINRIGYETMMELTGGRFKFIVATHTDKDHVHNHILINAIDRNSDKKFIWNYALERNLRMIS
DRISKMAGAKIIEKRYSYRGYKKYRESSHKFELKQRLYFLMQQSK

Specific function: Mediates initition of conjugal transfer of plasmid pRS01 possibly by introducing a single-stranded nick at the potential origin of transfer, to initiate single strand transfer from donor to recipient [H]

COG id: COG3843

COG function: function code U; Type IV secretory pathway, VirD2 components (relaxase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mobilization (MOB) protein type 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005094 [H]

Pfam domain/function: PF03432 Relaxase [H]

EC number: NA

Molecular weight: Translated: 24654; Mature: 24654

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLDFPSHTEMVEMYNVNFT
CEECCHHHHCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCEEHEEECCCC
NNDKLYESRNDRQEKHQQTIHAHHLIQSFSPEDNLTPEEINRIGYETMMELTGGRFKFIV
CCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEE
ATHTDKDHVHNHILINAIDRNSDKKFIWNYALERNLRMISDRISKMAGAKIIEKRYSYRG
EECCCHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
YKKYRESSHKFELKQRLYFLMQQSK
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLDFPSHTEMVEMYNVNFT
CEECCHHHHCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCEEHEEECCCC
NNDKLYESRNDRQEKHQQTIHAHHLIQSFSPEDNLTPEEINRIGYETMMELTGGRFKFIV
CCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEE
ATHTDKDHVHNHILINAIDRNSDKKFIWNYALERNLRMISDRISKMAGAKIIEKRYSYRG
EECCCHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
YKKYRESSHKFELKQRLYFLMQQSK
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655550; 10532373 [H]