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The map label for this gene is spxB [H]

Identifier: 116516119

GI number: 116516119

Start: 656976

End: 658751

Strand: Direct

Name: spxB [H]

Synonym: SPD_0636

Alternate gene names: 116516119

Gene position: 656976-658751 (Clockwise)

Preceding gene: 116517177

Following gene: 116517078

Centisome position: 32.11

GC content: 43.02

Gene sequence:

>1776_bases
ATGACTCAAGGGAAAATTACTGCATCTGCAGCAATGCTTAACGTATTGAAAACATGGGGCGTAGATACAATCTACGGTAT
CCCATCAGGAACACTCAGCTCATTGATGGACGCTTTGGCTGAAGACAAAGATATCCGCTTCTTACAAGTTCGCCACGAAG
AGACAGGTGCTCTTGCAGCGGTTATGCAAGCTAAATTCGGCGGCTCAATCGGGGTTGCAGTTGGTTCAGGTGGTCCAGGT
GCGACTCACTTGATTAACGGTGTTTACGATGCAGCTATGGATAACACTCCATTCCTAGCGATCCTTGGATCACGTCCAGT
TAACGAATTGAACATGGATGCTTTCCAAGAGCTTAACCAAAACCCAATGTACAACGGTATCGCTGTTTACAACAAACGTG
TAGCTTACGCTGAGCAATTGCCAAAAGTAATTGACGAAGCCTGCCGTGCTGCAATTTCTAAAAAAGGTCCAGCTGTTGTT
GAAATTCCAGTAAACTTCGGTTTCCAAGAAATCGACGAAAACTCATACTACGGTTCAGGTTCATACGAACGCTCATTCAT
CGCTCCTGCTTTGAACGAAGTTGAAATCGACAAAGCTGTTGAAATCTTGAACAATGCTGAACGCCCAGTTATCTATGCTG
GATTTGGTGGTGTTAAAGCTGGTGAAGTGATTACTGAATTGTCACGTAAAATCAAAGCACCAATCATCACAACTGGTAAA
AACTTTGAAGCTTTCGAATGGAACTATGAAGGTTTGACAGGTTCTGCTTACCGTGTTGGTTGGAAACCAGCCAACGAAGT
GGTCTTTGAAGCAGACACAGTTCTTTTCCTTGGTTCAAACTTCGCATTTGCTGAAGTTTACGAAGCATTCAAGAACACTG
AAAAATTCATACAAGTCGATATCGACCCTTACAAACTTGGTAAACGTCATGCCCTTGACGCTTCAATCCTTGGTGATGCT
GGTCAAGCAGCTAAAGCTATCCTTGACAAAGTAAACCCAGTTGAATCAACTCCATGGTGGCGTGCAAACGTTAAGAACAA
CCAAAACTGGCGTGATTACATGAACAAACTCGAAGGTAAAACTGAGGGTGAATTGCAATTGTATCAAGTTTACAATGCAA
TCAACAAACATGCTGATCAAGACGCTATCTACTCAATCGACGTAGGTAACACTACTCAAACATCTACTCGTCACCTTCAC
ATGACACCTAAGAATATGTGGCGTACATCTCCGCTCTTTGCGACAATGGGTATTGCCCTTCCTGGTGGTATCGCTGCTAA
GAAAGACAATCCAGATCGCCAAGTATGGAACATCATGGGTGATGGAGCATTCAACATGTGCTACCCAGACGTTATCACAA
ACGTTCAATACGACCTTCCAGTTATCAACCTTGTCTTCTCAAATGCTGAGTACGGCTTCATCAAGAACAAATACGAAGAT
ACAAACAAACACTTGTTTGGTGTAGACTTCACAAACGCTGACTACGCTAAAATTGCGGAAGCTCAAGGAGCTGTTGGATT
CACAGTTGACCGTATCGAAGACATCGATGCAGTTGTTGCAGAAGCTGTTAAATTGAACAAAGAAGGTAAAACTGTTGTCA
TCGATGCTCGCATCACTCAACACCGTCCACTTCCAGTAGAAGTACTTGAATTGGATCCAAAACTTCACTCAGAAGAAGCT
ATCAAAGCCTTCAAGGAAAAATACGAAGCAGAAGAACTCGTACCATTCCGTCTCTTCTTGGAAGAAGAAGGATTGCAATC
ACGCGCAATTAAATAA

Upstream 100 bases:

>100_bases
ATAAATATGATACAGTGGAATAGTAAAAATTTGGAGAACGTTTCCAATTCTATGTAATCGTATTCTCCAAGTTTAAAAAA
ATTGAAGGAGAGTTATCATT

Downstream 100 bases:

>100_bases
TTCCTCTCGCCGAAAATCAAATATGAAACTTGTAGACTTTATTCTTTGATTTTCATAGAGCAGAACTTTAAAAGATCGGA
GCAATCCGGTCTTTTTATGG

Product: pyruvate oxidase

Products: NA

Alternate protein names: Pyruvic oxidase; POX [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKDIRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPG
ATHLINGVYDAAMDNTPFLAILGSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGVKAGEVITELSRKIKAPIITTGK
NFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLFLGSNFAFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDA
GQAAKAILDKVNPVESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTTQTSTRHLH
MTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPDVITNVQYDLPVINLVFSNAEYGFIKNKYED
TNKHLFGVDFTNADYAKIAEAQGAVGFTVDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEA
IKAFKEKYEAEELVPFRLFLEEEGLQSRAIK

Sequences:

>Translated_591_residues
MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKDIRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPG
ATHLINGVYDAAMDNTPFLAILGSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGVKAGEVITELSRKIKAPIITTGK
NFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLFLGSNFAFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDA
GQAAKAILDKVNPVESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTTQTSTRHLH
MTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPDVITNVQYDLPVINLVFSNAEYGFIKNKYED
TNKHLFGVDFTNADYAKIAEAQGAVGFTVDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEA
IKAFKEKYEAEELVPFRLFLEEEGLQSRAIK
>Mature_590_residues
TQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKDIRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGA
THLINGVYDAAMDNTPFLAILGSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVVE
IPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGVKAGEVITELSRKIKAPIITTGKN
FEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLFLGSNFAFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAG
QAAKAILDKVNPVESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTTQTSTRHLHM
TPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPDVITNVQYDLPVINLVFSNAEYGFIKNKYEDT
NKHLFGVDFTNADYAKIAEAQGAVGFTVDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAI
KAFKEKYEAEELVPFRLFLEEEGLQSRAIK

Specific function: Important for the aerobic growth. Decarboxylates pyruvate in four steps. The energy released is partially stored in acetyl phosphate [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=552, Percent_Identity=23.9130434782609, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI21361361, Length=569, Percent_Identity=23.3743409490334, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI1787096, Length=557, Percent_Identity=28.3662477558348, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI1790104, Length=552, Percent_Identity=28.804347826087, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1786717, Length=561, Percent_Identity=25.3119429590018, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI87081685, Length=537, Percent_Identity=25.3258845437616, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1788716, Length=551, Percent_Identity=23.4119782214156, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17531299, Length=563, Percent_Identity=23.4458259325044, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17531301, Length=563, Percent_Identity=23.4458259325044, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17542570, Length=566, Percent_Identity=23.321554770318, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323755, Length=587, Percent_Identity=24.7018739352641, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320816, Length=560, Percent_Identity=24.4642857142857, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19922626, Length=555, Percent_Identity=24.6846846846847, Blast_Score=122, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014092
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.3.3 [H]

Molecular weight: Translated: 65255; Mature: 65123

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKDIRFLQVRHEETGALAA
CCCCCCHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHH
VMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAILGSRPVNELNMDAFQELNQ
HHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHCCC
NPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVVEIPVNFGFQEIDENSYYGSG
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCCCCCCCCC
SYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGVKAGEVITELSRKIKAPIITTGK
CCCCCHHCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEECCC
NFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLFLGSNFAFAEVYEAFKNTEKFIQVD
CCEEEEEECCCCCCCEEEECCCCCCCEEEECCEEEEECCCCHHHHHHHHHCCCCEEEEEE
IDPYKLGKRHALDASILGDAGQAAKAILDKVNPVESTPWWRANVKNNQNWRDYMNKLEGK
CCHHHCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHCCC
TEGELQLYQVYNAINKHADQDAIYSIDVGNTTQTSTRHLHMTPKNMWRTSPLFATMGIAL
CCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEECHHHHCCCCCHHHHHCEEC
PGGIAAKKDNPDRQVWNIMGDGAFNMCYPDVITNVQYDLPVINLVFSNAEYGFIKNKYED
CCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCEECCCCCC
TNKHLFGVDFTNADYAKIAEAQGAVGFTVDRIEDIDAVVAEAVKLNKEGKTVVIDARITQ
CCCEEEEEECCCCCHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECC
HRPLPVEVLELDPKLHSEEAIKAFKEKYEAEELVPFRLFLEEEGLQSRAIK
CCCCCEEEEECCCCCCHHHHHHHHHHHHCHHHCCEEEEEEECCCCHHCCCC
>Mature Secondary Structure 
TQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKDIRFLQVRHEETGALAA
CCCCCHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHH
VMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAILGSRPVNELNMDAFQELNQ
HHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHCCC
NPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVVEIPVNFGFQEIDENSYYGSG
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCCCCCCCCC
SYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGVKAGEVITELSRKIKAPIITTGK
CCCCCHHCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEECCC
NFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLFLGSNFAFAEVYEAFKNTEKFIQVD
CCEEEEEECCCCCCCEEEECCCCCCCEEEECCEEEEECCCCHHHHHHHHHCCCCEEEEEE
IDPYKLGKRHALDASILGDAGQAAKAILDKVNPVESTPWWRANVKNNQNWRDYMNKLEGK
CCHHHCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHCCC
TEGELQLYQVYNAINKHADQDAIYSIDVGNTTQTSTRHLHMTPKNMWRTSPLFATMGIAL
CCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEECHHHHCCCCCHHHHHCEEC
PGGIAAKKDNPDRQVWNIMGDGAFNMCYPDVITNVQYDLPVINLVFSNAEYGFIKNKYED
CCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCEECCCCCC
TNKHLFGVDFTNADYAKIAEAQGAVGFTVDRIEDIDAVVAEAVKLNKEGKTVVIDARITQ
CCCEEEEEECCCCCHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECC
HRPLPVEVLELDPKLHSEEAIKAFKEKYEAEELVPFRLFLEEEGLQSRAIK
CCCCCEEEEECCCCCCHHHHHHHHHHHHCHHHCCEEEEEEECCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8820650; 11463916 [H]