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The map label for this gene is rnjB [H]

Identifier: 116516084

GI number: 116516084

Start: 545228

End: 546889

Strand: Direct

Name: rnjB [H]

Synonym: SPD_0533

Alternate gene names: 116516084

Gene position: 545228-546889 (Clockwise)

Preceding gene: 116517143

Following gene: 116516440

Centisome position: 26.65

GC content: 43.5

Gene sequence:

>1662_bases
ATGAGTAATATCAGTTTAACAACACTTGGTGGTGTGCGTGAGAATGGAAAAAATATGTACATTGCTGAAATTGGAGAGTC
CATTTTTGTTTTGAATGTAGGGTTAAAATATCCTGAAAATGAACAATTAGGGGTCGATGTGGTGATTCCAAACATGGATT
ACCTTTTTGAAAATAGCGACCGTATTGCTGGGGTTTTCTTGACCCACGGGCATGCGGATGCCATTGGTGCTCTACCTTAT
CTCTTGGCAGAGGCTAAAGTTCCTGTATTTGGGTCTGAGTTGACCATTGAGTTGGCAAAGCTCTTTGTCAAAGGAAATGA
TGCCGTTAAGAAATTTAATGATTTCCATGTCATTGATGAGAATACGGAGATTGATTTTGGTGGGACAGTGGTTTCCTTCT
TCCCTACGACTTACTCCGTTCCAGAGAGTCTGGGAATTGTCTTGAAGACATCGGAAGGAAGCATCGTTTATACAGGTGAC
TTCAAATTTGACCAAACGGCTAGTGAATCTTATGCAACTGATTTTGCTCGTTTGGCAGAGATTGGTCGTGACGGCGTCCT
GGCTCTCCTCAGTGATTCGGCCAATGCAGACAGCAATATTCAGGTGGCTAGTGAAAGTGAAGTTAGGGATGAAATTACCC
AAACTATTGCTGACTGGGAAGGTCGTATCATCGTTGCAGCTGTTTCCAGTAATCTTTCTCGTATTCAGCAGATTTTTGAC
GCTGCGGATAAAACAGGTCGACGTATCGTCTTGACAGGATTTGATATTGAAAATATCGTCCGCACAGCGATTCGTCTTAA
GAAGTTGTCTTTAGCCAACGAAATTCTCTTGATTAAGCCTAAAGATATGTCTCGCTTTGAAGACCATGAGTTGATTATTC
TTGAGACAGGTCGTATGGGTGAACCTATCAATGGACTTCGTAAGATGTCGATTGGTCGCCATCGTTATGTAGAAATCAAG
GATGGGGACCTGGTCTATATTGCTACGGCTCCGTCTATTGCTAAAGAAGCCTTTGTTGCGCGTGTGGAAAATATGATTTA
TCAGGCAGGTGGGGTTGTCAAATTGATTACCCAAAGTTTACATGTATCAGGGCACGGAAATGTGCGTGATTTGCAGCTGA
TGATCAATCTTTTGCAACCTAAGTACCTCTTCCCTGTCCAAGGGGAGTATCGTGAGTTGGATGCTCACGCTAAGGCTGCC
ATGGCAGTTGGGATGTTGCCAGAACGCATCTTCATTCCTAAAAAGGGGACGACCATGGCTTACGAGAATGGAGACTTTGT
TCCAGCTGGATCGGTTTCAGCAGGAGATATCTTGATTGATGGGAATGCCATTGGTGATGTTGGAAATGTTGTTCTTCGTG
ACCGTAAGGTCTTGTCAGAGGATGGAATTTTCATCGTGGCTATTACAGTCAACCGTCGTGAGAAGAAAATTGTGGCTAGG
GCTCGTGTTCACACGCGTGGATTTGTTTATCTCAAGAAGAGTCGCGATATTCTCCGTGAAAGTTCAGAATTGATTAACCA
AACGGTAGAAGAGTATCTTCAAGGAGATGACTTTGACTGGGCAGATCTCAAAGGTAAGGTTCGTGACAATCTGACCAAGT
ACCTCTTTGATCAAACCAAGCGTCGCCCAGCCATTTTACCAGTAGTCATGGAAGCAAAATAA

Upstream 100 bases:

>100_bases
TATAATGGTAGGAAAAGATTCGGCTGAAAGTATCAGAACTTTTAGAATAAGAGGGTAGAATTGCCCTATAATCAAGATAA
ACTAAGATTTTGGAGGAAAA

Downstream 100 bases:

>100_bases
TCGTTGAAATAAACAGAGAGAAAGTCGAGTTTCGGCTTTTTCTTATAGAAAAATAGAAGGAGAAAATCATGGCAGTAATG
AAAATCGAGTATTACTCACA

Product: metallo-beta-lactamase superfamily protein

Products: NA

Alternate protein names: RNase J2 [H]

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLGVDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPY
LLAEAKVPVFGSELTIELAKLFVKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIADWEGRIIVAAVSSNLSRIQQIFD
AADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEILLIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIK
DGDLVYIATAPSIAKEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRELDAHAKAA
MAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNVVLRDRKVLSEDGIFIVAITVNRREKKIVAR
ARVHTRGFVYLKKSRDILRESSELINQTVEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK

Sequences:

>Translated_553_residues
MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLGVDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPY
LLAEAKVPVFGSELTIELAKLFVKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIADWEGRIIVAAVSSNLSRIQQIFD
AADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEILLIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIK
DGDLVYIATAPSIAKEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRELDAHAKAA
MAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNVVLRDRKVLSEDGIFIVAITVNRREKKIVAR
ARVHTRGFVYLKKSRDILRESSELINQTVEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK
>Mature_552_residues
SNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLGVDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYL
LAEAKVPVFGSELTIELAKLFVKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGDF
KFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIADWEGRIIVAAVSSNLSRIQQIFDA
ADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEILLIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKD
GDLVYIATAPSIAKEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRELDAHAKAAM
AVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNVVLRDRKVLSEDGIFIVAITVNRREKKIVARA
RVHTRGFVYLKKSRDILRESSELINQTVEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Appears to have a limited effect on 16S rRNA maturation, despite its similarity to rnjA [H]

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61167; Mature: 61036

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLGVDVVIPNMDYLFENSD
CCCCEEEEECCCEECCCEEEEEECCCEEEEEEECCCCCCCCCCEEEEEECCCHHEECCCC
RIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLFVKGNDAVKKFNDFHVIDE
CEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHEEEHHHCCCCHHHHCCCCEEEEEC
NTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGDFKFDQTASESYATDFARLAE
CCEEECCCEEEEECCCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHH
IGRDGVLALLSDSANADSNIQVASESEVRDEITQTIADWEGRIIVAAVSSNLSRIQQIFD
CCCCCEEEEEECCCCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHH
AADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEILLIKPKDMSRFEDHELIILETGRMG
HHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCCC
EPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIAKEAFVARVENMIYQAGGVVKLITQSL
CCHHHHHHHCCCCEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHEEEHHHH
HVSGHGNVRDLQLMINLLQPKYLFPVQGEYRELDAHAKAAMAVGMLPERIFIPKKGTTMA
EECCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEE
YENGDFVPAGSVSAGDILIDGNAIGDVGNVVLRDRKVLSEDGIFIVAITVNRREKKIVAR
ECCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHCCHHHCCCCEEEEEEEECCCHHHHHHH
ARVHTRGFVYLKKSRDILRESSELINQTVEEYLQGDDFDWADLKGKVRDNLTKYLFDQTK
HEEEECEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHC
RRPAILPVVMEAK
CCCEEEEEEEECC
>Mature Secondary Structure 
SNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLGVDVVIPNMDYLFENSD
CCCEEEEECCCEECCCEEEEEECCCEEEEEEECCCCCCCCCCEEEEEECCCHHEECCCC
RIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLFVKGNDAVKKFNDFHVIDE
CEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHEEEHHHCCCCHHHHCCCCEEEEEC
NTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGDFKFDQTASESYATDFARLAE
CCEEECCCEEEEECCCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHH
IGRDGVLALLSDSANADSNIQVASESEVRDEITQTIADWEGRIIVAAVSSNLSRIQQIFD
CCCCCEEEEEECCCCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHH
AADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEILLIKPKDMSRFEDHELIILETGRMG
HHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCCC
EPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIAKEAFVARVENMIYQAGGVVKLITQSL
CCHHHHHHHCCCCEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHEEEHHHH
HVSGHGNVRDLQLMINLLQPKYLFPVQGEYRELDAHAKAAMAVGMLPERIFIPKKGTTMA
EECCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEE
YENGDFVPAGSVSAGDILIDGNAIGDVGNVVLRDRKVLSEDGIFIVAITVNRREKKIVAR
ECCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHCCHHHCCCCEEEEEEEECCCHHHHHHH
ARVHTRGFVYLKKSRDILRESSELINQTVEEYLQGDDFDWADLKGKVRDNLTKYLFDQTK
HEEEECEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHC
RRPAILPVVMEAK
CCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]