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The map label for this gene is rsuA-1 [H]

Identifier: 116516048

GI number: 116516048

Start: 258091

End: 258813

Strand: Direct

Name: rsuA-1 [H]

Synonym: SPD_0260

Alternate gene names: 116516048

Gene position: 258091-258813 (Clockwise)

Preceding gene: 116516350

Following gene: 116516913

Centisome position: 12.61

GC content: 44.67

Gene sequence:

>723_bases
ATGAGATTAGATAAATTTTTAGTTGCCTGTGCGGTGGGAAGTCGGACTGAGGTCAAAAACTTGCTCAAGGCTGGGCGCGT
GACTGTAAATGGTAAAAAAGAAAAATCAGCTAAATTTCAGATTGATGAAAAAATAGATGAGATTCGCTTTGATGGGCAAG
TGTTGGAGTATGAAGAGTTTGTCTACTACATGATGAACAAGCCCAAAGGAGTTATCTCAGCGACTGAGGATCCCAAGCAC
AGAACCGTTCTGGACTTGTTGGATGACTTGGCGCGGAGTAAGGAAGTTTTCCCAGTAGGACGCTTGGATATTAACACGCA
TGGTCTTTTGCTCTTGACCAATGATGGTCAGCTGGCTCATGTTCTTCTTTCGCCCAAGCGTCATGTGGACAAGACTTATC
TGGCACAAGTCAAGGGAATCATGACCCAAGAAGATGTGGAGACATTTGCTGAGGGTATTCCTCTCAAAGACTTTACCTGT
CAGCCCGCTAGACTGGAGATTCTTTCCACAGATGCAGAAAAGAATCAGAGCCAAATCCGTGTGACCATTGCAGAAGGGAA
GTTTCATCAGATCAAGCGTATGGTGGGCTACTGTGGCAAGGAAGTGGTGGACTTGCAACGATTGACTATGGGAACTTTAG
TATTAGATGAGAACCTGGAACGAGGGGAATGGCGTCGCTTGACCAAGGAAGAATTAGAAATTCTCCGCGCTAATATTATT
TAA

Upstream 100 bases:

>100_bases
AGGCGACGTTGACGCGGTTTGAAGAGATTTTTGAAGAGTATGAAACGAAAAGGAGGTTGGTCATTGTACCAGCCTCCTTT
TGATATGATATAATAGTTCT

Downstream 100 bases:

>100_bases
TTTAGTTTAATACTCAATGAAAATCAAAGAGCAAACTAGGAAACTAGCCGCAGGCTGTACTTGAGTACGGCAAGGCGACG
TTGACGTGGTTTGAAGAGAT

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKFQIDEKIDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKH
RTVLDLLDDLARSKEVFPVGRLDINTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDENLERGEWRRLTKEELEILRANII

Sequences:

>Translated_240_residues
MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKFQIDEKIDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKH
RTVLDLLDDLARSKEVFPVGRLDINTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDENLERGEWRRLTKEELEILRANII
>Mature_240_residues
MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKFQIDEKIDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKH
RTVLDLLDDLARSKEVFPVGRLDINTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDENLERGEWRRLTKEELEILRANII

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=236, Percent_Identity=40.2542372881356, Blast_Score=170, Evalue=8e-44,
Organism=Escherichia coli, GI1787524, Length=246, Percent_Identity=32.9268292682927, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1790453, Length=238, Percent_Identity=30.672268907563, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87081838, Length=178, Percent_Identity=33.7078651685393, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27384; Mature: 27384

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKFQIDEKIDEIRFDGQVLEYEEF
CCHHHEEHHHHCCCHHHHHHHHHHCCEEECCCCCCCCCEEHHHHHHHHCCCCEEEHHHHH
VYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRLDINTHGLLLLTNDGQLAH
HHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCEEE
VLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTCQPARLEILSTDAEKNQSQIR
EEECCHHHCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCEEE
VTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDENLERGEWRRLTKEELEILRANII
EEEECCHHHHHHHHHHHHCHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKFQIDEKIDEIRFDGQVLEYEEF
CCHHHEEHHHHCCCHHHHHHHHHHCCEEECCCCCCCCCEEHHHHHHHHCCCCEEEHHHHH
VYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRLDINTHGLLLLTNDGQLAH
HHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCEEE
VLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTCQPARLEILSTDAEKNQSQIR
EEECCHHHCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCEEE
VTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDENLERGEWRRLTKEELEILRANII
EEEECCHHHHHHHHHHHHCHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]