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The map label for this gene is pfkA [H]

Identifier: 116515997

GI number: 116515997

Start: 806708

End: 807715

Strand: Direct

Name: pfkA [H]

Synonym: SPD_0789

Alternate gene names: 116515997

Gene position: 806708-807715 (Clockwise)

Preceding gene: 116516844

Following gene: 116516870

Centisome position: 39.43

GC content: 44.35

Gene sequence:

>1008_bases
ATGAAACGTATTGCTGTTTTGACTAGTGGCGGAGACGCACCTGGTATGAACGCTGCCATCCGTGCAGTTGTTCGTCAAGC
AATTTCAGAAGGAATGGAAGTGTTTGGTATCTATGACGGATATGCTGGTATGGTTGCCGGTGAAATTCATCCCCTAGATG
CAGCTTCAGTAGGGGACATCATTTCTCGTGGTGGTACTTTTCTTCACTCAGCTCGTTATCCAGAGTTCGCTCAACTTGAA
GGGCAACTTAAAGGGATTGAGCAATTGAAAAAACACGGAATTGAAGGTGTAGTTGTTATCGGTGGTGACGGATCTTACCA
CGGCGCTATGCGTTTGACTGAACATGGCTTCCCAGCTATTGGTCTTCCAGGTACAATCGATAACGATATCGTTGGTACTG
ACTTTACAATCGGTTTTGACACAGCGGTTACTACTGCCATGGACGCTATCGATAAGATTCGTGATACATCATCAAGTCAC
CGTCGTACTTTTGTAATCGAAGTTATGGGACGTAACGCTGGTGATATCGCTCTTTGGGCTGGTATTGCAACTGGTGCTGA
TGAAATCATCATCCCTGAAGCAGGCTTCAAGATGGAAGATATCGTAGCAAGCATCAAAGCTGGTTATGAATGTGGTAAAA
AACACAATATCATCGTCTTAGCTGAAGGTGTGATGTCAGCGGCTGAATTTGGTCAAAAACTTAAAGAAGCTGGAGATATA
AGCGACCTTCGTGTAACAGAACTTGGACATATTCAACGTGGTGGTTCTCCAACTCCGCGTGACCGTGTTTTGGCGTCACG
TATGGGTGCACATGCTGTTAAACTTCTTAAAGAAGGTATCGGTGGTGTTGCGGTTGGTATTCGTAACGAAAAAATGGTTG
AAAATCCAATTCTTGGTACTGCAGAAGAAGGGGCATTGTTTAGCCTTACTGCAGAAGGTAAGATTGTGGTTAACAACCCA
CACGAAGCCGATATTGAGCTATCTAGCTTGAATAAGAGCTTGTCATAA

Upstream 100 bases:

>100_bases
AAAACGATTTATCGCTAAAAATACGGAAAATAGAAGAATTTTAAAATCAAATGTGATATAATCAAGAAGAATGTTAAAAG
AAAAAGGAGCATAAACCAAT

Downstream 100 bases:

>100_bases
TTTACAATTTAGTTAATAAGACCAAAAAGGTCATATATATAAAGGAGTCACAAAAATCATGAACAAACGTGTAAAAATCG
TTGCAACTTTGGGTCCTGCG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGMVAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLE
GQLKGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNIIVLAEGVMSAAEFGQKLKEAGDI
SDLRVTELGHIQRGGSPTPRDRVLASRMGAHAVKLLKEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNP
HEADIELSSLNKSLS

Sequences:

>Translated_335_residues
MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGMVAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLE
GQLKGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNIIVLAEGVMSAAEFGQKLKEAGDI
SDLRVTELGHIQRGGSPTPRDRVLASRMGAHAVKLLKEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNP
HEADIELSSLNKSLS
>Mature_335_residues
MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGMVAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLE
GQLKGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNIIVLAEGVMSAAEFGQKLKEAGDI
SDLRVTELGHIQRGGSPTPRDRVLASRMGAHAVKLLKEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNP
HEADIELSSLNKSLS

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=312, Percent_Identity=42.9487179487179, Blast_Score=208, Evalue=5e-54,
Organism=Homo sapiens, GI266453768, Length=315, Percent_Identity=41.9047619047619, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI266453748, Length=315, Percent_Identity=41.9047619047619, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI4505749, Length=315, Percent_Identity=41.9047619047619, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI48762920, Length=316, Percent_Identity=42.0886075949367, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI266453619, Length=337, Percent_Identity=40.0593471810089, Blast_Score=201, Evalue=6e-52,
Organism=Escherichia coli, GI1790350, Length=303, Percent_Identity=58.7458745874587, Blast_Score=343, Evalue=7e-96,
Organism=Caenorhabditis elegans, GI25147584, Length=343, Percent_Identity=39.6501457725948, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17558788, Length=310, Percent_Identity=35.8064516129032, Blast_Score=175, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI72003601, Length=343, Percent_Identity=30.6122448979592, Blast_Score=155, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6321679, Length=337, Percent_Identity=39.1691394658754, Blast_Score=200, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6323861, Length=344, Percent_Identity=37.5, Blast_Score=186, Evalue=5e-48,
Organism=Drosophila melanogaster, GI28573326, Length=338, Percent_Identity=40.5325443786982, Blast_Score=216, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24652337, Length=338, Percent_Identity=40.5325443786982, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI17647809, Length=339, Percent_Identity=40.4129793510324, Blast_Score=216, Evalue=3e-56,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35214; Mature: 35214

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGMVAGEIHPLDAASVGDI
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCEECCCCCCCCHHHHHH
ISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAI
HHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCC
GLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSHRRTFVIEVMGRNAGDIALWA
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEE
GIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNIIVLAEGVMSAAEFGQKLKEAGDI
EECCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHCCC
SDLRVTELGHIQRGGSPTPRDRVLASRMGAHAVKLLKEGIGGVAVGIRNEKMVENPILGT
CCEEEHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCCCCCCC
AEEGALFSLTAEGKIVVNNPHEADIELSSLNKSLS
CCCCCEEEEECCCEEEECCCCCCCEEHHHHCCCCC
>Mature Secondary Structure
MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGMVAGEIHPLDAASVGDI
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCEECCCCCCCCHHHHHH
ISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAI
HHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCC
GLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSHRRTFVIEVMGRNAGDIALWA
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEE
GIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNIIVLAEGVMSAAEFGQKLKEAGDI
EECCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHCCC
SDLRVTELGHIQRGGSPTPRDRVLASRMGAHAVKLLKEGIGGVAVGIRNEKMVENPILGT
CCEEEHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCCCCCCC
AEEGALFSLTAEGKIVVNNPHEADIELSSLNKSLS
CCCCCEEEEECCCEEEECCCCCCCEEHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA