Click here to switch to the map view.

The map label for this gene is rnhB

Identifier: 116515974

GI number: 116515974

Start: 1044680

End: 1045459

Strand: Direct

Name: rnhB

Synonym: SPD_1020

Alternate gene names: 116515974

Gene position: 1044680-1045459 (Clockwise)

Preceding gene: 116515446

Following gene: 116517148

Centisome position: 51.06

GC content: 41.28

Gene sequence:

>780_bases
ATGGCAACGATTAAAGAAATCAAAGAACTCCTTGTGACAGTCAAGGAGTTAGAAAGCCCTATTTTTTTAGAGCTTGAAAA
GGATAATCGCTCAGGAGTTCAAAAGGAAATCAGCAAGCGTAAAAGAGCCATTCAAGCTGAATTAGATGAAAATTTGCGCT
TGGAATCCATGCTTTCTTATGAAAAAGAACTTTATAAGCAAGGATTGACCTTAATTGTAGGTATTGATGAGGTTGGTCGT
GGTCCTCTTGCTGGTCCTGTAGTCGCTGCGGCCGTCATTTTACCTAAAAATTGTAAGATTAAAGGTCTCAACGACAGCAA
GAAAATTCCTAAAAAGAAACATCTGGAGATTTTCCAAGCCGTTCAAGACCAAGCCTTGTCGATTGGAATTGGTATCATAG
ATAATCAGGTCATCGACCAAGTCAACATCTATGAAGCAACCAAACTAGCCATGCAAGAAGCAATCTCCCAGCTCAGCCCT
CAACCAGAGCACCTTTTGATTGATGCCATGAAACTGGACTTGCCCATTTCACAAACCTCCATTATCAAAGGAGATGCCAA
CTCCCTCTCTATCGCAGCAGCATCTATAGTAGCCAAGGTAACACGTGATGAATTGATGAAAGAATACGATCAGCAGTTCC
CTGGCTATGATTTCGCTACTAATGCAGGATATGGCACAGCTAAACATCTGGAAGGCCTCACAAAACTAGGAGTTACCCCA
ATTCACCGAACCAGCTTTGAACCCGTTAAATCACTGGTTTTAGGTAAAAAAGAAAGTTAA

Upstream 100 bases:

>100_bases
GGTTTCCGTGATGACTATGACCGTTTTTACAGTCTCTTCGTGAAGGAAGTCCGTGATGGCAAACTCGGTAACTATACCTT
AGATACATTGGAAGACCTCG

Downstream 100 bases:

>100_bases
TTGAAAGGAAATACCATGGAGGAACAGTCGGAAATAGTCCGTTCTAAGAAAGAATTCGCCTTTGCATCCAGCACTATACT
ATCCCAAGTTGGTCGAGGAA

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAIQAELDENLRLESMLSYEKELYKQGLTLIVGIDEVGR
GPLAGPVVAAAVILPKNCKIKGLNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDFATNAGYGTAKHLEGLTKLGVTP
IHRTSFEPVKSLVLGKKES

Sequences:

>Translated_259_residues
MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAIQAELDENLRLESMLSYEKELYKQGLTLIVGIDEVGR
GPLAGPVVAAAVILPKNCKIKGLNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDFATNAGYGTAKHLEGLTKLGVTP
IHRTSFEPVKSLVLGKKES
>Mature_258_residues
ATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAIQAELDENLRLESMLSYEKELYKQGLTLIVGIDEVGRG
PLAGPVVAAAVILPKNCKIKGLNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSPQ
PEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDFATNAGYGTAKHLEGLTKLGVTPI
HRTSFEPVKSLVLGKKES

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=181, Percent_Identity=49.171270718232, Blast_Score=167, Evalue=6e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_STRP2 (Q04KF4)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816494.1
- ProteinModelPortal:   Q04KF4
- SMR:   Q04KF4
- STRING:   Q04KF4
- EnsemblBacteria:   EBSTRT00000019651
- GeneID:   4442425
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1020
- eggNOG:   COG0164
- GeneTree:   EBGT00050000028203
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 28515; Mature: 28383

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAIQAELDENLRLESMLSY
CCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
EKELYKQGLTLIVGIDEVGRGPLAGPVVAAAVILPKNCKIKGLNDSKKIPKKKHLEIFQA
HHHHHHCCCEEEEECHHCCCCCCCHHHHHHHHHCCCCCEEECCCCHHHCCHHHHHHHHHH
VQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSPQPEHLLIDAMKLDLPISQTS
HHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHH
IIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDFATNAGYGTAKHLEGLTKLGVTP
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCC
IHRTSFEPVKSLVLGKKES
CCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
ATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAIQAELDENLRLESMLSY
CCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
EKELYKQGLTLIVGIDEVGRGPLAGPVVAAAVILPKNCKIKGLNDSKKIPKKKHLEIFQA
HHHHHHCCCEEEEECHHCCCCCCCHHHHHHHHHCCCCCEEECCCCHHHCCHHHHHHHHHH
VQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSPQPEHLLIDAMKLDLPISQTS
HHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHH
IIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDFATNAGYGTAKHLEGLTKLGVTP
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCC
IHRTSFEPVKSLVLGKKES
CCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA