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The map label for this gene is dut [H]

Identifier: 116515930

GI number: 116515930

Start: 24776

End: 25219

Strand: Direct

Name: dut [H]

Synonym: SPD_0027

Alternate gene names: 116515930

Gene position: 24776-25219 (Clockwise)

Preceding gene: 116516620

Following gene: 116516859

Centisome position: 1.21

GC content: 44.59

Gene sequence:

>444_bases
ATGAAAATTCGTGGTTTTGAATTGGTTTCGAGTTTTACAGATGAAAATTTATTGCCCAAGCGTGAGACAGCGCATGCGGC
TGGTTACGACTTAAAGGTTGCTGTGCGTACAGTTGTTGCGCCAGGAGAGATTGTCTTGGTTCCGACAGGGGTTAAGGCTT
ATATGCAGCCGACTGAGGTTCTCTACCTCTATGATCGTTCTTCAAATCCTCGTAAGAAGGGCTTGGTTTTAATTAACTCA
GTTGGGGTCATTGATGGGGATTATTATGGAAATCCTGGAAATGAAGGGCATATTTTTGCGCAGATGAAGAATATCACAGA
CCAAGAGGTTGTTCTTGAAGTTGGGGAGCGTATTGTCCAGGCTGTTTTTGCTACTTTCTTAATTGCAGATGGAGATGCAG
CTGATGGCGTTCGAACTGGTGGATTTGGATCGACAGGGCACTAG

Upstream 100 bases:

>100_bases
AGCCCTAAGCGATTTGAATTGTGTGCTCTTTTTTTCGTGCTTTTTCCGAATAAATAAGATAGAATAATCTAGAATAAATG
ATAATAGAAAAGAGAAAATT

Downstream 100 bases:

>100_bases
AATGAAGATTATCTTTGTACGTCATGGGGAGCCAGATTACCGTGAGTTAGAGGAGCGTTCTTATATAGGATTTGGGATAG
ATTTGGCACCCTTGTCTGAG

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase [H]

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEIVLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINS
VGVIDGDYYGNPGNEGHIFAQMKNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH

Sequences:

>Translated_147_residues
MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEIVLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINS
VGVIDGDYYGNPGNEGHIFAQMKNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH
>Mature_147_residues
MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEIVLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINS
VGVIDGDYYGNPGNEGHIFAQMKNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [H]

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family [H]

Homologues:

Organism=Escherichia coli, GI1790071, Length=138, Percent_Identity=36.231884057971, Blast_Score=67, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008180 [H]

Pfam domain/function: PF00692 dUTPase [H]

EC number: =3.6.1.23 [H]

Molecular weight: Translated: 15843; Mature: 15843

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEIVLVPTGVKAYMQPTEV
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCCHHHCCCCEE
LYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQMKNITDQEVVLEVGERIVQ
EEEEECCCCCCCCCEEEEEECCEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHH
AVFATFLIADGDAADGVRTGGFGSTGH
HHHHHHEEECCCCCCCEECCCCCCCCC
>Mature Secondary Structure
MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEIVLVPTGVKAYMQPTEV
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCCHHHCCCCEE
LYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQMKNITDQEVVLEVGERIVQ
EEEEECCCCCCCCCEEEEEECCEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHH
AVFATFLIADGDAADGVRTGGFGSTGH
HHHHHHEEECCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]