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The map label for this gene is hsdS [H]

Identifier: 116515880

GI number: 116515880

Start: 458167

End: 459447

Strand: Direct

Name: hsdS [H]

Synonym: SPD_0451

Alternate gene names: 116515880

Gene position: 458167-459447 (Clockwise)

Preceding gene: 116516122

Following gene: 116516914

Centisome position: 22.39

GC content: 29.9

Gene sequence:

>1281_bases
GTGAGGATAAAATCAATTTATTGGAATTTTGGGCAAAATAAGCCAGAGAAATCCTTTAGGTATATAGATACGTCTAGTAT
TGATAGAAAAAAGAACATAATCAACTACAAAAATCTACAATATCTTTCACCTGAACAAGCGCCTTCCCGTGCTAGAAAAT
TAGTTTCGCAGAATAGTGTCTTATTTTCAACAGTTAGACCATATCTAAAAAATATTGCTGTAGTTAGAGAACTTAAAGAG
TATTTGATAGCTAGTACAGCATTTATTGTTTTGGATACTTTACTTAACGAAACATATTTGAAATATTATTTATTATCAGA
TAATTTTATCAATCGTGTTAATAATAAATCTACTGGAACAAGTTATCCTGCAATCAATGATTATAATTTTAATCTATTAT
TAATTGCTCTCCCCCCACTATCCGAACAACAACGAATAGTAGAAGCAATCGAATCAGCTTTAGAAAAAGTAGATGAATAT
GCTGAAAGTTATAATAGACTAGAACAGCTAGATAAAGAATTTCCAGATAAACTAAAAAAATCTATTCTTCAATATGCTAT
GCAAGGAAAATTAGTTGAACAAGACCCAAATGATGAATCAGTCGAAGTTTTACTTGAAAAAATACGAGCAGAAAAACAAA
AACTATTTGAAGAAGGCAAGATTAAAAAGAAAGATTTGGACATTTCTATTGTTTCCCAAGGAGATGATAACTCTTATTAT
GGGAACAAAGACGAAACAACCTCTTATCCTATATATGAAATCCCAGAAGCATGGAGATATATTAAATTTGCTAGCCTAGT
TAACTTCAGAATAGGAAAAACTCCTCCACGCAGTGAAGCTACTTTTTGGGGAACTGAAATACCTTGGGTATCTATATCAG
ATATGCCTATATCTGGTTATGTAACTAATACGAGAGAATCTATTTCTAAATTAGCATTAAAATCAAAGAAAATAGACATT
TCACCTAAAGGAACTTTATTAATGAGTTTTAAATTATCTATTGGAAAAGTTGCCATATTGGATATTCCTGCCACTCATAA
TGAAGCCATTATATCGATATTCCCCTATGCTAATAAAGAAAATATTATCAGAGATTACTTAATGATATTTTTGCCACTTA
TCTCTACTTTAGGTGATTCAAAAGATGCTATCAAAGGGAAAACATTAAATAGTACCAGTATCTCCGAATTATTAATCCCT
ATTTCTAATCATGAAGAGATGAAAAGAATCATTTTCAAAGTTGATTTACTTTTTCAAAAAGTTTCTCAACTTTTTGAGTA
A

Upstream 100 bases:

>100_bases
TTATATACTTGAAGCACATAAGAAACATTATGGGAAGTTTGCTGATGGAAGCACTCAAGAAATTGATGTTCCTTATGATA
TTCCTGATACTTGGGAGTGG

Downstream 100 bases:

>100_bases
TAAGTTCCTGTTCCTCAAAAGGAGCTAACGGAATTAATAGCTCGCTCAGTGTAGTTTTAGGAATATTATATAAAGCTTGA
CCTGATAGTTTAGTTATTGC

Product: type I restriction-modification system, S subunit

Products: NA

Alternate protein names: S.EcoAI; Type I restriction enzyme EcoAI specificity protein; S protein [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MRIKSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLKNIAVVRELKE
YLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLSEQQRIVEAIESALEKVDEY
AESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYY
GNKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTEIPWVSISDMPISGYVTNTRESISKLALKSKKIDI
SPKGTLLMSFKLSIGKVAILDIPATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIP
ISNHEEMKRIIFKVDLLFQKVSQLFE

Sequences:

>Translated_426_residues
MRIKSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLKNIAVVRELKE
YLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLSEQQRIVEAIESALEKVDEY
AESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYY
GNKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTEIPWVSISDMPISGYVTNTRESISKLALKSKKIDI
SPKGTLLMSFKLSIGKVAILDIPATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIP
ISNHEEMKRIIFKVDLLFQKVSQLFE
>Mature_426_residues
MRIKSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLKNIAVVRELKE
YLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLSEQQRIVEAIESALEKVDEY
AESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYY
GNKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTEIPWVSISDMPISGYVTNTRESISKLALKSKKIDI
SPKGTLLMSFKLSIGKVAILDIPATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIP
ISNHEEMKRIIFKVDLLFQKVSQLFE

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 48987; Mature: 48987

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIKSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSV
CCCCEEEEECCCCCCCCCEEEECCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCH
LFSTVRPYLKNIAVVRELKEYLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHCCCCCCCC
SYPAINDYNFNLLLIALPPLSEQQRIVEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYY
HHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
GNKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTEIPWVSISDMPISGY
CCCCCCCCCCCEECHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEECCCCCCCE
VTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIPATHNEAIISIFPYANKE
ECCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCEEEEEECCCCHH
NIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPISNHEEMKRIIFKVDLLFQK
HHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHEEECCCHHHHHHHHHHHHHHHHH
VSQLFE
HHHHCC
>Mature Secondary Structure
MRIKSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSV
CCCCEEEEECCCCCCCCCEEEECCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCH
LFSTVRPYLKNIAVVRELKEYLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHCCCCCCCC
SYPAINDYNFNLLLIALPPLSEQQRIVEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYY
HHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
GNKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTEIPWVSISDMPISGY
CCCCCCCCCCCEECHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEECCCCCCCE
VTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIPATHNEAIISIFPYANKE
ECCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCEEEEEECCCCHH
NIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPISNHEEMKRIIFKVDLLFQK
HHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHEEECCCHHHHHHHHHHHHHHHHH
VSQLFE
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2642743 [H]