Click here to switch to the map view.

The map label for this gene is folD [H]

Identifier: 116515868

GI number: 116515868

Start: 735934

End: 736812

Strand: Direct

Name: folD [H]

Synonym: SPD_0721

Alternate gene names: 116515868

Gene position: 735934-736812 (Clockwise)

Preceding gene: 116515734

Following gene: 116517034

Centisome position: 35.97

GC content: 45.51

Gene sequence:

>879_bases
TTGAAAGAAAAGAGAAGTGATATGACACAGATTATTGATGGGAAAGCTTTAGCGGCCAAATTGCAGGGGCAGTTGGCTGA
AAAGACTGCAAAATTAAAGGAAGAAACAGCTCTAGTGCCTGGTTTGGTAGTGATTTTGGTTGGGGATAATCCAGCCAGCC
AAGTCTACGTTCGCAACAAGGAGAGGTCAGCTCTTGCGGCTGGTTCCCGTAGCGAAGTAGTGCGAGTTCCAGAGACCATT
ACTCAAGAGGAATTGTTAGACCTGATTGCTAAATATAATCAGGATCCAGCTTGGCATGGGATTTTGGTCCAGTTGCCATT
ACCAAAACATATCGATGAAGAGGCGGTTTTATTAGCCATTGACCCAGAAAAGGATGTGGATGGTTTCCATCCCCTAAACA
TGGGACGTCTTTGGTCTGGACATCCAGTCATGATTCCCTCGACACCTGCAGGAATTATGGAAATGTTTCATGAATATGGG
ATTGACTTGGAAGGTAAAAATGCGGTCGTAATCGGTCGTTCAAATATCGTTGGAAAACCTATGGCTCAGCTTCTTTTGGC
TAAAAATGCGACAGTAACCTTGACCCACTCACGAACTCATAATCTTGCCAAGGTGGCTGCTAAAGCAGATATTCTTGTAG
TCGCAATCGGCCGCGCCAAGTTTGTGACTGCTGACTTTGTCAAACCAGGTGCGGTTGTCATTGACGTTGGGATGAATCGA
GATGAAAATGGGAAGCTCTGTGGAGATGTTGATTATGAGGCAGTTGCACCACTTGCTAGCCATATCACGCCAGTCCCTGG
AGGTGTCGGTCCTATGACCATTACCATGCTGATGGAGCAAACTTATCAAGCAGCACTTCGGACATTGGATAGAAAATAA

Upstream 100 bases:

>100_bases
AAAGTTCAACCAGAGCTTTTTCTTATAGTTTGAAACTATAGGATAACCTAGGAAAGAAGTGTTAGAGGGCACTAGCAGTT
TTTGGTATAATGGAAGATAT

Downstream 100 bases:

>100_bases
GAGAAGCATTCGATTAATAGAGTGTATTTTCTATAGCTATATCTAAAATGGCAGAAATGAATATTAAATTTTAGATATAA
GATTACAAAAGGAGGTCTGC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKEKRSDMTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNPASQVYVRNKERSALAAGSRSEVVRVPETI
TQEELLDLIAKYNQDPAWHGILVQLPLPKHIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYG
IDLEGKNAVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTADFVKPGAVVIDVGMNR
DENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQAALRTLDRK

Sequences:

>Translated_292_residues
MKEKRSDMTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNPASQVYVRNKERSALAAGSRSEVVRVPETI
TQEELLDLIAKYNQDPAWHGILVQLPLPKHIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYG
IDLEGKNAVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTADFVKPGAVVIDVGMNR
DENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQAALRTLDRK
>Mature_292_residues
MKEKRSDMTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNPASQVYVRNKERSALAAGSRSEVVRVPETI
TQEELLDLIAKYNQDPAWHGILVQLPLPKHIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYG
IDLEGKNAVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTADFVKPGAVVIDVGMNR
DENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQAALRTLDRK

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=293, Percent_Identity=43.0034129692833, Blast_Score=232, Evalue=3e-61,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=46.2585034013605, Blast_Score=232, Evalue=3e-61,
Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=45.9183673469388, Blast_Score=221, Evalue=6e-58,
Organism=Homo sapiens, GI36796743, Length=214, Percent_Identity=26.1682242990654, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1786741, Length=281, Percent_Identity=50.1779359430605, Blast_Score=271, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI17568735, Length=291, Percent_Identity=42.6116838487972, Blast_Score=209, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=42.6174496644295, Blast_Score=241, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6319558, Length=289, Percent_Identity=43.9446366782007, Blast_Score=239, Evalue=5e-64,
Organism=Drosophila melanogaster, GI17136816, Length=302, Percent_Identity=47.682119205298, Blast_Score=257, Evalue=5e-69,
Organism=Drosophila melanogaster, GI17136818, Length=300, Percent_Identity=47.6666666666667, Blast_Score=255, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24645718, Length=292, Percent_Identity=46.5753424657534, Blast_Score=242, Evalue=3e-64,
Organism=Drosophila melanogaster, GI17137370, Length=292, Percent_Identity=46.5753424657534, Blast_Score=242, Evalue=3e-64,
Organism=Drosophila melanogaster, GI62472483, Length=292, Percent_Identity=46.5753424657534, Blast_Score=241, Evalue=3e-64,
Organism=Drosophila melanogaster, GI45551871, Length=292, Percent_Identity=46.5753424657534, Blast_Score=241, Evalue=3e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31555; Mature: 31555

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKRSDMTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNPASQVYVRNK
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECC
ERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPKHIDEEAVLLAI
CCHHHHCCCCCCEEECCHHCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEE
DPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKNAVVIGRSNIVGKP
CCCCCCCCCCCCCCCCEECCCEEEECCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCH
MAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTADFVKPGAVVIDVGMNR
HHHHHHHCCCEEEEEECCCCHHHHHHHCCCEEEEEECCCEEEEECCCCCCEEEEEECCCC
DENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQAALRTLDRK
CCCCCEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKEKRSDMTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNPASQVYVRNK
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECC
ERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPKHIDEEAVLLAI
CCHHHHCCCCCCEEECCHHCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEE
DPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKNAVVIGRSNIVGKP
CCCCCCCCCCCCCCCCEECCCEEEECCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCH
MAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTADFVKPGAVVIDVGMNR
HHHHHHHCCCEEEEEECCCCHHHHHHHCCCEEEEEECCCEEEEECCCCCCEEEEEECCCC
DENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQAALRTLDRK
CCCCCEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA