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The map label for this gene is ppnK

Identifier: 116515865

GI number: 116515865

Start: 995239

End: 996057

Strand: Direct

Name: ppnK

Synonym: SPD_0983

Alternate gene names: 116515865

Gene position: 995239-996057 (Clockwise)

Preceding gene: 116515750

Following gene: 116516162

Centisome position: 48.64

GC content: 41.39

Gene sequence:

>819_bases
ATGAAGAATACAGGTAAACGAATTGATCTGATAGCCAATAGAAAACCGCAGAGTCAAAGGGTTTTGTATGAATTGCGAGA
TCGTTTGAAGAGAAATCAGTTTATACTCAATGATACCAATCCGGATATTGTCATTTCCATTGGCGGGGATGGTATGCTCT
TGTCGGCCTTTCATAAGTACGAAAATCAGCTTGACAAGGTCCGCTTTATCGGTCTTCATACTGGACATTTGGGCTTCTAT
ACAGATTATCGTGATTTTGAGTTGGACAAGCTAGTGACTAATTTGCAACTAGATACTGGGGCAAGGGTTTCTTACCCTGT
TCTGAATGTGAAGGTCTTTCTTGAAAATGGTGAAGTTAAGATTTTCAGAGCACTCAACGAAGCCAGCATCCGCAGGTCTG
ATCGAACCATGGTGGCAGATATTGTAATAAATGGTGTTCCCTTTGAACGTTTTCGTGGAGACGGGCTAACAGTTTCGACA
CCGACTGGTAGTACTGCCTATAACAAGTCTCTTGGCGGTGCTGTTTTACACCCTACCATTGAAGCTTTGCAATTAACGGA
GATTGCCAGCCTTAATAATCGTGTCTATCGAACATTGGGCTCTTCCATTATTGTGCCTAAGAAGGATAAGATTGAACTTA
TTCCAACAAGAAACGATTATCATACTATTTCGGTTGACAATAGCGTTTATTCTTTCCGTAATATTGAGCGTATTGAGTAT
CAAATCGACCATCATAAGATTCACTTTGTCGCGACTCCTAGCCATACCAGTTTCTGGAACCGTGTTAAGGATGCCTTTAT
CGGTGAGGTGGATGAATGA

Upstream 100 bases:

>100_bases
GAAATGGGTGAAATTCGTGATGATATCCAAGAAGCCCAGGCACTTTTTGATCCTTTGAGTAGAAAATTAAATGACGGTGT
AGGAAACAGTGACGATACAG

Downstream 100 bases:

>100_bases
GGTTTGAATTTATCGCAGATGAACATGTCAAGGTTAAGACCTTTTTAAAAAAGCACGAGGTTTCTAAGGGATTGCTGGCC
AAGATTAAGTTTCGAGGTGG

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIVISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFY
TDYRDFELDKLVTNLQLDTGARVSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRNDYHTISVDNSVYSFRNIERIEY
QIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE

Sequences:

>Translated_272_residues
MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIVISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFY
TDYRDFELDKLVTNLQLDTGARVSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRNDYHTISVDNSVYSFRNIERIEY
QIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE
>Mature_272_residues
MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIVISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFY
TDYRDFELDKLVTNLQLDTGARVSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRNDYHTISVDNSVYSFRNIERIEY
QIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Escherichia coli, GI1788968, Length=223, Percent_Identity=26.457399103139, Blast_Score=66, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322509, Length=273, Percent_Identity=26.7399267399267, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325068, Length=163, Percent_Identity=30.6748466257669, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320794, Length=199, Percent_Identity=26.6331658291457, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_STRP2 (Q04KI9)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816459.1
- ProteinModelPortal:   Q04KI9
- SMR:   Q04KI9
- STRING:   Q04KI9
- EnsemblBacteria:   EBSTRT00000018993
- GeneID:   4441101
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0983
- eggNOG:   COG0061
- GeneTree:   EBGT00050000026927
- HOGENOM:   HBG314020
- OMA:   YINNEFL
- ProtClustDB:   PRK04885
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 31036; Mature: 31036

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIVISIGGDGMLLSAFHKY
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCEEEEECCCCCEEEEECCCCEEHHHHHHH
ENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGARVSYPVLNVKVFLENGEVK
HHHHCEEEEEEEEECCEEEECCCCCCCHHHHHHHEEECCCCEEEEEEEEEEEEEECCCEE
IFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVSTPTGSTAYNKSLGGAVLHPTI
EEEECCHHHHCCCCCEEEEEEEECCCCHHHCCCCCEEEECCCCCCCCCCCCCCEEECCHH
EALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRNDYHTISVDNSVYSFRNIERIEY
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCEEEEEECCCEEHHCCEEEEEE
QIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE
EECCEEEEEEECCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIVISIGGDGMLLSAFHKY
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCEEEEECCCCCEEEEECCCCEEHHHHHHH
ENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGARVSYPVLNVKVFLENGEVK
HHHHCEEEEEEEEECCEEEECCCCCCCHHHHHHHEEECCCCEEEEEEEEEEEEEECCCEE
IFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVSTPTGSTAYNKSLGGAVLHPTI
EEEECCHHHHCCCCCEEEEEEEECCCCHHHCCCCCEEEECCCCCCCCCCCCCCEEECCHH
EALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRNDYHTISVDNSVYSFRNIERIEY
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCEEEEEECCCEEHHCCEEEEEE
QIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE
EECCEEEEEEECCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA