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The map label for this gene is ackA

Identifier: 116515857

GI number: 116515857

Start: 1836335

End: 1837525

Strand: Reverse

Name: ackA

Synonym: SPD_1853

Alternate gene names: 116515857

Gene position: 1837525-1836335 (Counterclockwise)

Preceding gene: 116516707

Following gene: 116516968

Centisome position: 89.81

GC content: 40.39

Gene sequence:

>1191_bases
ATGACAAAAACAATTGCAATCAATGCAGGAAGTTCAAGTTTGAAATGGCAATTATACTTAATGCCAGAAGAAAAAGTATT
GGCGAAAGGTTTGATTGAACGTATCGGTTTGAAAGATTCAATTTCAACTGTAAAATTTGACGGCCGTTCTGAACAACAAA
TTTTGGATATTGAAAATCATATACAAGCCGTTAAAATTTTATTGGATGACTTGATTCGTTTCGATATTATCAAGGCTTAT
GACGAGATTACAGGTGTTGGACATCGTGTTGTTGCTGGTGGAGAATATTTCAAAGAATCAACAGTTGTTGAGGGAGATGT
TTTAGAAAAAGTTGAAGAGTTGAGTTTGTTGGCTCCTCTACACAACCCGGCCAATGCAGCAGGTGTTCGTGCCTTCAAGG
AATTGTTGCCAGACATTACCAGTGTAGTTGTTTTTGATACTTCCTTCCACACAAGTATGCCAGAGAAAGCTTATCGCTAC
CCTCTACCAACAAAATATTACACAGAAAACAAGGTTCGTAAATACGGTGCTCATGGTACAAGTCACCAGTTTGTAGCAGG
AGAAGCTGCAAAACTCTTGGGACGTCCATTAGAAGACTTGAAGTTAATTACCTGTCATATTGGTAACGGAGGCTCAATTA
CAGCTGTGAAAGCCGGCAAATCTGTAGACACTTCTATGGGGTTCACTCCTCTTGGTGGTATTATGATGGGAACGCGTACA
GGGGATATTGATCCAGCTATCATTCCTTATTTAATGCAATATACAGAGGATTTTAACACACCAGAAGATATCAGTCGTGT
TCTTAACCGTGAATCAGGTCTTTTGGGAGTTTCTGCTAATTCTAGCGATATGCGCGATATAGAAGCAGCTGTAGCAGAAG
GGAATCACGAGGCTAGCTTGGCTTATGAAATGTATGTTGACCGTATCCAAAAACATATCGGTCAGTACCTTGCAGTGCTA
AATGGAGCAGATGCCATTGTTTTCACAGCAGGTGTCGGTGAAAATGCAGAGAGTTTCCGTCGTGATGTAATCTCAGGGAT
TTCGTGGTTTGGTTGTGATGTTGATGATGAAAAGAATGTCTTTGGCGTTACAGGAGACATCTCAACAGAGGCAGCTAAAA
TCCGTGTCTTGGTTATTCCAACAGATGAAGAATTAGTCATTGCCCGTGACGTTGAACGCTTGAAAAAATAA

Upstream 100 bases:

>100_bases
TGAAATTTAAAGAAAATTTTCAAAAATGGACTCAAGGTACTGAAATATAAAATAGATTTTGTTATAATAGTTGAAAACGC
TTAAAAAGGGGTATCATGTT

Downstream 100 bases:

>100_bases
GTGAAACTAAAAAAATATTCAATACAAGGAGTTGGGAAAGTTATTTTTCCAGCTTCTTTTTCTGATGAAATTGTCCAAAA
CCTTGCTATGATTGGCTTTT

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTVKFDGRSEQQILDIENHIQAVKILLDDLIRFDIIKAY
DEITGVGHRVVAGGEYFKESTVVEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKAGKSVDTSMGFTPLGGIMMGTRT
GDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLGVSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVL
NGADAIVFTAGVGENAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVERLKK

Sequences:

>Translated_396_residues
MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTVKFDGRSEQQILDIENHIQAVKILLDDLIRFDIIKAY
DEITGVGHRVVAGGEYFKESTVVEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKAGKSVDTSMGFTPLGGIMMGTRT
GDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLGVSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVL
NGADAIVFTAGVGENAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVERLKK
>Mature_395_residues
TKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTVKFDGRSEQQILDIENHIQAVKILLDDLIRFDIIKAYD
EITGVGHRVVAGGEYFKESTVVEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRYP
LPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKAGKSVDTSMGFTPLGGIMMGTRTG
DIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLGVSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLN
GADAIVFTAGVGENAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVERLKK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=397, Percent_Identity=41.3098236775819, Blast_Score=298, Evalue=6e-82,
Organism=Escherichia coli, GI145693184, Length=395, Percent_Identity=40.7594936708861, Blast_Score=288, Evalue=3e-79,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_STRP2 (Q04IC8)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_817260.1
- ProteinModelPortal:   Q04IC8
- SMR:   Q04IC8
- STRING:   Q04IC8
- EnsemblBacteria:   EBSTRT00000018914
- GeneID:   4441549
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1853
- eggNOG:   COG0282
- GeneTree:   EBGT00050000027501
- HOGENOM:   HBG285863
- OMA:   SSDFRDV
- ProtClustDB:   PRK00180
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 43343; Mature: 43212

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTVKFDGRSEQQILDIENH
CCEEEEEECCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHH
IQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTVVEGDVLEKVEELSLLAPL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCHHHHHHHHHHHHHHCC
HNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRYPLPTKYYTENKVRKYGAHGT
CCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHCCCCCCCCCCCHHHHHHCCCCC
SHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKAGKSVDTSMGFTPLGGIMMGTRT
CCCEECCHHHHHHCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEECCC
GDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLGVSANSSDMRDIEAAVAEGNHEASL
CCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCHHH
AYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGENAESFRRDVISGISWFGCDVDDEKNV
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCEECCCCCCCCCE
FGVTGDISTEAAKIRVLVIPTDEELVIARDVERLKK
EEEECCCCCCCEEEEEEEEECCCCEEHHHHHHHHCC
>Mature Secondary Structure 
TKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTVKFDGRSEQQILDIENH
CEEEEEECCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHH
IQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTVVEGDVLEKVEELSLLAPL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCHHHHHHHHHHHHHHCC
HNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRYPLPTKYYTENKVRKYGAHGT
CCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHCCCCCCCCCCCHHHHHHCCCCC
SHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKAGKSVDTSMGFTPLGGIMMGTRT
CCCEECCHHHHHHCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEECCC
GDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLGVSANSSDMRDIEAAVAEGNHEASL
CCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCHHH
AYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGENAESFRRDVISGISWFGCDVDDEKNV
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCEECCCCCCCCCE
FGVTGDISTEAAKIRVLVIPTDEELVIARDVERLKK
EEEECCCCCCCEEEEEEEEECCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA