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The map label for this gene is murD

Identifier: 116515762

GI number: 116515762

Start: 622902

End: 624254

Strand: Direct

Name: murD

Synonym: SPD_0598

Alternate gene names: 116515762

Gene position: 622902-624254 (Clockwise)

Preceding gene: 116516538

Following gene: 116515971

Centisome position: 30.44

GC content: 44.35

Gene sequence:

>1353_bases
ATGAAAGTAATAGATCAATTTAAAAATAAGAAAGTTCTTGTTTTAGGTTTGGCCAAGTCTGGTGAATCTGCAGCTCGTTT
GTTGGACAAGCTAGGTGCCATTGTGACAGTAAATGATGGGAAACCTTTCGAGGACAATCCAGCTGCCCAAAGTTTGCTGG
AAGAAGGGATCAAGGTCATTACAGGTGGCCATCCTTTGGAACTCTTGGATGAAGAGTTTGCCCTTATGGTGAAAAATCCA
GGTATCCCCTACAACAATCCCATGATTGAAAAGGCTTTGGCCAAGAGAATTCCAGTCTTGACTGAGGTGGAATTGGCTTA
TTTGATTTCAGAAGCACCGATTATTGGTATCACAGGATCGAACGGTAAGACAACCACAACGACTATGATTGGGGAAGTTT
TGACTGCTGCTGGGCAACATGGTCTTTTATCAGGGAATATCGGCTATCCTGCCAGTCAGGTTGCTCAAATAGCATCAGAT
AAGGACACGCTTGTTATGGAACTTTCTTCTTTCCAACTCATGGGTGTTCAAGAATTCCATCCAGAGATTGCGGTTATTAC
CAACCTCATGCCAACTCATATCGACTACCATGGGTCATTTTCTGAATATGTAGCAGCCAAGTGGAATATCCAGAACAAGA
TGACAGCAGCTGATTTCCTTGTCTTGAACTTTAATCAAGACTTGGCAAAAGACTTGACTTCCAAGACAGAAGCCACTGTT
GTACCATTTTCAACACTTGAAAAGGTTGATGGAGCTTATCTGGAAGATGGTCAACTCTACTTCCGTGGTGAAGTAGTCAT
GGCAGCGAATGAAATCGGTGTTCCAGGTAGCCACAATGTGGAAAATGCCCTTGCGACTATTGCTGTAGCCAAGCTTCGTG
ATGTGGACAATCAAACCATCAAGGAAACTCTTTCAGCCTTCGGTGGTGTCAAACACCGTCTCCAGTTTGTGGATGACATC
AAGGGTGTTAAATTCTATAACGACAGTAAATCAACTAATATCTTGGCTACTCAAAAAGCCTTATCAGGATTTGACAACAG
CAAGGTCGTCTTGATTGCAGGTGGTTTGGACCGTGGCAATGAGTTTGACGAATTGGTGCCAGACATTACTGGACTCAAGA
AGATGGTCATCCTGGGTCAATCTGCAGAACGTGTCAAACGGGCAGCAGACAAGGCTGGTGTCGCTTATGTGGAGGCGACA
GATATTGCAGATGCGACCCGCAAGGCCTATGAGCTTGCGACTCAAGGAGATGTGGTTCTTCTTAGTCCTGCCAATGCCAG
CTGGGATATGTATGCTAACTTTGAAGTACGTGGCGACCTCTTTATCGACACAGTAGCGGAGTTAAAAGAATAA

Upstream 100 bases:

>100_bases
GGTAAGATTCAGTTAGAAGAAAGAGTCACAAACACACTTTGTGGCTTTTTTATTTCCATAAAAATGGTAAAATAGTAGGA
GTAGAAATGGAGTTCGAGAC

Downstream 100 bases:

>100_bases
AATATGAAAAAAATTGTCTTTACAGGTGGGGGGACGGTTGGACACGTTACCCTCAATCTTTTGTTAATGCCCAAGTTCAT
CGAAGATGGTTGGGAAGTCC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFEDNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNP
GIPYNNPMIEKALAKRIPVLTEVELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAADFLVLNFNQDLAKDLTSKTEATV
VPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPGSHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDI
KGVKFYNDSKSTNILATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKAGVAYVEAT
DIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE

Sequences:

>Translated_450_residues
MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFEDNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNP
GIPYNNPMIEKALAKRIPVLTEVELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAADFLVLNFNQDLAKDLTSKTEATV
VPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPGSHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDI
KGVKFYNDSKSTNILATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKAGVAYVEAT
DIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
>Mature_450_residues
MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFEDNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNP
GIPYNNPMIEKALAKRIPVLTEVELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAADFLVLNFNQDLAKDLTSKTEATV
VPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPGSHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDI
KGVKFYNDSKSTNILATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKAGVAYVEAT
DIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=451, Percent_Identity=31.4855875831486, Blast_Score=161, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_STRP2 (Q04LK1)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816097.1
- ProteinModelPortal:   Q04LK1
- SMR:   Q04LK1
- STRING:   Q04LK1
- EnsemblBacteria:   EBSTRT00000020249
- GeneID:   4442418
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0598
- eggNOG:   COG0771
- GeneTree:   EBGT00050000027197
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK02472
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 48580; Mature: 48580

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFEDNPAAQSLLEEGIKVI
CCCHHHHCCCEEEEEEECCCCCHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCCCEEE
TGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKRIPVLTEVELAYLISEAPIIGITGS
ECCCCHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECC
NGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASDKDTLVMELSSFQLMGVQEFH
CCCEEHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHCCCCEEEEEECCCEEECHHHHC
PEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAADFLVLNFNQDLAKDLTSKTEATV
CCCEEECCCCCCCEECCCCHHHHEEEEECCCCCCEEEEEEEEECCHHHHHHHHCCCCEEE
VPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPGSHNVENALATIAVAKLRDVDNQTI
ECHHHHHHCCCCEEECCEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHH
KETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNILATQKALSGFDNSKVVLIAGGLDRGN
HHHHHHHCCHHHHHHHHHHCCCCEEECCCCCCEEEEEHHHHCCCCCCEEEEEECCCCCCC
EFDELVPDITGLKKMVILGQSAERVKRAADKAGVAYVEATDIADATRKAYELATQGDVVL
CHHHHCCCHHCCCEEEEECCCHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHCCCCEEE
LSPANASWDMYANFEVRGDLFIDTVAELKE
EECCCCCCEEEEEEEEECCCHHHHHHHHCC
>Mature Secondary Structure
MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFEDNPAAQSLLEEGIKVI
CCCHHHHCCCEEEEEEECCCCCHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCCCEEE
TGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKRIPVLTEVELAYLISEAPIIGITGS
ECCCCHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECC
NGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASDKDTLVMELSSFQLMGVQEFH
CCCEEHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHCCCCEEEEEECCCEEECHHHHC
PEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAADFLVLNFNQDLAKDLTSKTEATV
CCCEEECCCCCCCEECCCCHHHHEEEEECCCCCCEEEEEEEEECCHHHHHHHHCCCCEEE
VPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPGSHNVENALATIAVAKLRDVDNQTI
ECHHHHHHCCCCEEECCEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHH
KETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNILATQKALSGFDNSKVVLIAGGLDRGN
HHHHHHHCCHHHHHHHHHHCCCCEEECCCCCCEEEEEHHHHCCCCCCEEEEEECCCCCCC
EFDELVPDITGLKKMVILGQSAERVKRAADKAGVAYVEATDIADATRKAYELATQGDVVL
CHHHHCCCHHCCCEEEEECCCHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHCCCCEEE
LSPANASWDMYANFEVRGDLFIDTVAELKE
EECCCCCCEEEEEEEEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA