Click here to switch to the map view.

The map label for this gene is pnp [H]

Identifier: 116515732

GI number: 116515732

Start: 523226

End: 525553

Strand: Direct

Name: pnp [H]

Synonym: SPD_0512

Alternate gene names: 116515732

Gene position: 523226-525553 (Clockwise)

Preceding gene: 116516593

Following gene: 116516473

Centisome position: 25.57

GC content: 43.9

Gene sequence:

>2328_bases
TTGTCTTCATCGAAGACTTCGTCAGTTTCCTATTTTTACTTTGCTTTTGACGTCCTTGGTATCTTGATCTTTGTAGGCAA
GGCGTATAATTTCATCAATCCAAAGGGGATTAAAATGGCAAAACAAGTGTTTCAAACGACTTTTGCGGGTCGTGAGTTAA
TTGTAGAAACTGGTCAGGTTGCTAAGCAAGCAAATGGCTCTGTTGTCGTACGTTACGGTGAGTCAACTGTCTTGACTGCT
GCCGTTATGTCTAAGAAAATGGCAACTGGGGATTTCTTCCCACTCCAAGTCAACTACGAAGAAAAAATGTATGCGGCTGG
GAAGTTTCCTGGTGGCTTTATGAAACGTGAAGGACGTCCTTCAACAGATGCGACCTTGACAGCGCGTTTGATTGACCGTC
CGATTCGTCCTATGTTTGCGGAAGGTTTCCGTAATGAAGTCCAAGTCATCAATACAGTGCTTTCTTATGATGAAAATGCA
TCTGCACCAATGGCTGCTATGTTTGGTTCATCTTTGGCACTGTCTATTTCAGATATTCCATTTGACGGACCAATTGCTGG
GGTACAAGTGGGATATGTAGATGGCCAAATCATCATCAACCCAAGTCAAGAACAAGCAGAGCAATCTCTTCTTGAATTGA
CAGTAGCTGGAACCAAGCACGCTATCAACATGGTAGAGTCTGGTGCCAAAGAATTGTCAGAAGAAATCATGTTGGAAGCG
CTTCTTAAAGGGCACGAAGCTGTCAAAGAATTGATTGCCTTCCAAGAAGAAATCGTTGCTGCTGTCGGTAAAGAAAAAGC
AGAAGTGGAATTGCTTCACGTGGATGCTGAATTGCAAGCTGAAATCATTGCAGCCTACAACAGTGACCTCCAAAAGGCAG
TTCAAGTAGAAGAGAAATTGGCCCGTGAAGCTGCAACTCAAGCAGTGAAAGACCAAGTGACTGCCGTTTACGAAGAAAAA
TATGCGAACCACGAAGAATTTGACCGTATTATGCGTGATGTGGCTGAAATCTTGGAACAAATGGAACACGCAGAAGTGCG
ACGTTTAATTACAGAAGACAAGGTGCGTCCTGATGGTCGTAAGGTCGATGAAATCCGTCCTTTGGATGCGGTTGTTGACT
TCCTTCCTCGTGTACATGGTTCAGGTCTCTTTACTCGTGGGCAAACTCAAGCTCTTTCAGTCTTGACCTTGGCTCCGATG
GGAGAAACTCAAATCATTGATGGTTTGGATCCAGAGTACAAGAAACGCTTTATGCACCACTATAACTTCCCTCAATATTC
TGTAGGGGAAACAGGTCGTTACGGTGCGCCAGGTCGTCGTGAAATCGGTCACGGTGCCCTTGGTGAGCGTGCTCTTGCTC
AAGTCTTGCCAAGCTTGGAAGAATTCCCATACGCTATCCGTCTAGTAGCAGAAGTTTTGGAATCAAACGGTTCTTCATCT
CAAGCTTCTATCTGTGCGGGAACTCTTGCCCTTATGGCTGGTGGTGTGCCAATCAAGGCGCCAGTAGCTGGTATTGCTAT
GGGACTTATCTCAGATGGAAATAACTACACAGTATTGACAGATATCCAAGGTTTGGAAGATCACTTTGGAGATATGGACT
TCAAGGTTGCAGGTACTCGTGATGGGATTACAGCCCTTCAAATGGATATCAAGATTCAAGGGATTACTGCAGAAATCTTG
ACGGAGGCTCTTGCTCAAGCCAAGAAAGCGCGTTTTGAAATCCTTGATGTCATTGAAGCAACCATTCCAGAAGTTCGTCC
AGAATTGGCTCCAACTGCTCCGAAAATTGATACGATCAAGATTGATGTGGACAAGATTAAGATTGTCATCGGTAAGGGTG
GAGAAACCATCGACAAGATTATCGCTGAAACAGGTGTTAAGATTGATATAGACGAAGAAGGAAATGTGTCTATCTACTCT
AGTGACCAAGATGCTATTAACCGTGCCAAAGAAATTATTGCTGGTTTGGTTCGTGAAGCCAAAGTGGATGAAGTTTACCG
TGCTAAAGTCGTTCGTATCGAGAAATTTGGTGCCTTTGTTAACCTCTTTGATAAGACAGATGCCCTTGTTCATATCTCTG
AGATGGCTTGGACTCGTACCAATCGTGTAGAGGATTTGGTAGAAATCGGGGATGAAGTTGATGTTAAGGTTATCAAAATT
GATGAAAAAGGCCGTATCGATGCCTCTATGAAGGCTCTTCTACCTCGTCCGCCAAAACCTGAGCATGATGAAAAAGGTGA
AAAGTCTGAGCGCCCTCACCGCCCACGTCATCAAAAGGATTACAAACCTAAGAAAGAATTTACAGAAACACCAAAAGATT
CAGAATAA

Upstream 100 bases:

>100_bases
CTTCCTATTTTTCTACAGAAATGTTCGGCAAGCCGAACCGTCCAAAATATCTTGTGTAATTGAACACGGCCGAAAAGCTG
TGTAAAAAAGATAAACTGTC

Downstream 100 bases:

>100_bases
GAAAAGGAGAAATGTATGGGGTGGTGGCGCGAAACCATTGATATTGTAAAAGAAAATGATCCAGCGGCCCGCACCACTTT
GGAGGTTTTGCTGACTTATC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 775; Mature: 774

Protein sequence:

>775_residues
MSSSKTSSVSYFYFAFDVLGILIFVGKAYNFINPKGIKMAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTA
AVMSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDENA
SAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQIIINPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEA
LLKGHEAVKELIAFQEEIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVTAVYEEK
YANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVVDFLPRVHGSGLFTRGQTQALSVLTLAPM
GETQIIDGLDPEYKKRFMHHYNFPQYSVGETGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSS
QASICAGTLALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITALQMDIKIQGITAEIL
TEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVDKIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYS
SDQDAINRAKEIIAGLVREAKVDEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDYKPKKEFTETPKDSE

Sequences:

>Translated_775_residues
MSSSKTSSVSYFYFAFDVLGILIFVGKAYNFINPKGIKMAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTA
AVMSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDENA
SAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQIIINPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEA
LLKGHEAVKELIAFQEEIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVTAVYEEK
YANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVVDFLPRVHGSGLFTRGQTQALSVLTLAPM
GETQIIDGLDPEYKKRFMHHYNFPQYSVGETGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSS
QASICAGTLALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITALQMDIKIQGITAEIL
TEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVDKIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYS
SDQDAINRAKEIIAGLVREAKVDEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDYKPKKEFTETPKDSE
>Mature_774_residues
SSSKTSSVSYFYFAFDVLGILIFVGKAYNFINPKGIKMAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAA
VMSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDENAS
APMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQIIINPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEAL
LKGHEAVKELIAFQEEIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVTAVYEEKY
ANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVVDFLPRVHGSGLFTRGQTQALSVLTLAPMG
ETQIIDGLDPEYKKRFMHHYNFPQYSVGETGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQ
ASICAGTLALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITALQMDIKIQGITAEILT
EALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVDKIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSS
DQDAINRAKEIIAGLVREAKVDEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKID
EKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDYKPKKEFTETPKDSE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=714, Percent_Identity=34.3137254901961, Blast_Score=405, Evalue=1e-113,
Organism=Escherichia coli, GI145693187, Length=689, Percent_Identity=48.6211901306241, Blast_Score=615, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI115534063, Length=720, Percent_Identity=34.1666666666667, Blast_Score=357, Evalue=2e-98,
Organism=Drosophila melanogaster, GI281362905, Length=697, Percent_Identity=36.441893830703, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651641, Length=697, Percent_Identity=36.441893830703, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651643, Length=697, Percent_Identity=36.441893830703, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI161079377, Length=661, Percent_Identity=35.7034795763994, Blast_Score=398, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 85241; Mature: 85110

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSKTSSVSYFYFAFDVLGILIFVGKAYNFINPKGIKMAKQVFQTTFAGRELIVETGQV
CCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
AKQANGSVVVRYGESTVLTAAVMSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRP
HHHCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCHHHHEECCCCCCCHHCCCCCC
STDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIP
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEEEECCC
FDGPIAGVQVGYVDGQIIINPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEA
CCCCCCCEEEEEECCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LLKGHEAVKELIAFQEEIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKL
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
AREAATQAVKDQVTAVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGR
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KVDEIRPLDAVVDFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHH
CHHHCCCHHHHHHHHHHHCCCCCEECCCHHHEEEEEECCCCCCCEECCCCHHHHHHHHHH
YNFPQYSVGETGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCC
QASICAGTLALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTR
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHEECCCCEEEEEECCCHHHHCCCCCEEEECCC
DGITALQMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIK
CCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
IDVDKIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREA
EEEEEEEEEEECCCHHHHHHHHHCCCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHH
KVDEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDYKPKKEFTETPKDSE
CCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure 
SSSKTSSVSYFYFAFDVLGILIFVGKAYNFINPKGIKMAKQVFQTTFAGRELIVETGQV
CCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
AKQANGSVVVRYGESTVLTAAVMSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRP
HHHCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCHHHHEECCCCCCCHHCCCCCC
STDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIP
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEEEECCC
FDGPIAGVQVGYVDGQIIINPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEA
CCCCCCCEEEEEECCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LLKGHEAVKELIAFQEEIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKL
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
AREAATQAVKDQVTAVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGR
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KVDEIRPLDAVVDFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHH
CHHHCCCHHHHHHHHHHHCCCCCEECCCHHHEEEEEECCCCCCCEECCCCHHHHHHHHHH
YNFPQYSVGETGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCC
QASICAGTLALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTR
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHEECCCCEEEEEECCCHHHHCCCCCEEEECCC
DGITALQMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIK
CCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
IDVDKIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREA
EEEEEEEEEEECCCHHHHHHHHHCCCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHH
KVDEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDYKPKKEFTETPKDSE
CCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA