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The map label for this gene is argG

Identifier: 116515666

GI number: 116515666

Start: 109320

End: 110516

Strand: Direct

Name: argG

Synonym: SPD_0110

Alternate gene names: 116515666

Gene position: 109320-110516 (Clockwise)

Preceding gene: 116516177

Following gene: 116516947

Centisome position: 5.34

GC content: 41.77

Gene sequence:

>1197_bases
ATGAGTAAAGAAAAAGTAATTTTGGCCTATTCTGGCGGTTTAGACACATCAGTTGCTATTACCTGGCTGAAGAAAGACTA
TGATGTTGTTGCAGTTTGTATGGATGTGGGTGAAGGGAAAGACTTAGATTTCATCCATGATAAGGCTCTTAAGGTTGGGG
CAGTCGAATCTTATGTCATTGATGTTAAGGACGAATTTGCTACAGATTATGTGCTAGTGGCTCATCAGTCACATGCCTAT
TATGAACAAAAGTATCCCTTGGTATCTGCCTTGAGCCGCCCTCTGATTTCTAAAAAATTGGTTGAAATAGCGCATCAGAT
AGGAGCGACTACAATTGCTCATGGTTGTACCGGTAAGGGGAATGACCAAGTACGTTTTGAAGTATCGATTGCAGCCTTGG
ATCTCAATTTAAAAGTGATTGCTCCAGTTCGTGAATGGAAATGGTCTCGGGAGGAAGAAATTTATTATGCCAAGGAAAAT
GGGGTGCCTGTTCCTGCTGACCTTGACAATCCCTACTCTGTCGACCAAAATCTTTGGGGACGTGCCAATGAGTGTGGTAT
TTTGGAAAATCCATGGAATCAGGCTCCAGAAGAAGCCTTTGGCATCACAACTTCTCCAGAGCAAGCTCCAGATATGCCAG
AATACATTGAGATTGAGTTTAGTGAAGGTGTTCCAGTTTCCCTCAATGGAGAAGTGTTAAAATTGGCAGATTTGATTCAA
AAACTCAATGAAATAGCTGGAAAACATGGGGTTGGACGGATTGACCACGTGGAAAATCGCTTGGTTGGGATTAAATCAAG
AGAAATCTATGAGTGTCCAGGTGCAGTGACCCTGCTGACAGCTCATAAGGAAATTGAGGACTTGACGCTTGTGAGAGAAG
TGGCTCATTTTAAACCAATCATAGAAAATGAATTATCAAATCTCATTTATAATGCTTTGTGGTTTAGTTCGGCTACACAA
GCCTTGATTGCTTATATTAAAGAGACTCAAAAAGTTGTCAATGGGACTGCAAAAGTCAAACTGTATAAGGGGAGCGCTCA
GGTCGTGGCTCGGAAATCACCAAGTTCTCTTTACGATGAAAATTTGGCGACGTATACTAGTGCAGATACCTTTGACCAAG
ATGCGGCTGTTGGCTTTATTAAGTTATGGGGACTTCCAACCAAGGTTCATTCTGAGGTTCAAAAAAGCGCCAAATAG

Upstream 100 bases:

>100_bases
GATAAAACCATTCAAAAGTTGAAGGAGTCTGGGGAATTAGACAAACTCATTGAGGATGCCTTTAAAGCGTCCATTGAAAA
ATAGAAAGAAGGAAAAGAAC

Downstream 100 bases:

>100_bases
GACGATTGGATAGAGAGGTGGATGTGATATGGCTAAGAATACAAAATTATGGGGTGGTCGCTTTGAAGGTACTGTCGAAG
ACTGGGTAGAGCGCTTTGGT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSKEKVILAYSGGLDTSVAITWLKKDYDVVAVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVAHQSHAY
YEQKYPLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKGNDQVRFEVSIAALDLNLKVIAPVREWKWSREEEIYYAKEN
GVPVPADLDNPYSVDQNLWGRANECGILENPWNQAPEEAFGITTSPEQAPDMPEYIEIEFSEGVPVSLNGEVLKLADLIQ
KLNEIAGKHGVGRIDHVENRLVGIKSREIYECPGAVTLLTAHKEIEDLTLVREVAHFKPIIENELSNLIYNALWFSSATQ
ALIAYIKETQKVVNGTAKVKLYKGSAQVVARKSPSSLYDENLATYTSADTFDQDAAVGFIKLWGLPTKVHSEVQKSAK

Sequences:

>Translated_398_residues
MSKEKVILAYSGGLDTSVAITWLKKDYDVVAVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVAHQSHAY
YEQKYPLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKGNDQVRFEVSIAALDLNLKVIAPVREWKWSREEEIYYAKEN
GVPVPADLDNPYSVDQNLWGRANECGILENPWNQAPEEAFGITTSPEQAPDMPEYIEIEFSEGVPVSLNGEVLKLADLIQ
KLNEIAGKHGVGRIDHVENRLVGIKSREIYECPGAVTLLTAHKEIEDLTLVREVAHFKPIIENELSNLIYNALWFSSATQ
ALIAYIKETQKVVNGTAKVKLYKGSAQVVARKSPSSLYDENLATYTSADTFDQDAAVGFIKLWGLPTKVHSEVQKSAK
>Mature_397_residues
SKEKVILAYSGGLDTSVAITWLKKDYDVVAVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVAHQSHAYY
EQKYPLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKGNDQVRFEVSIAALDLNLKVIAPVREWKWSREEEIYYAKENG
VPVPADLDNPYSVDQNLWGRANECGILENPWNQAPEEAFGITTSPEQAPDMPEYIEIEFSEGVPVSLNGEVLKLADLIQK
LNEIAGKHGVGRIDHVENRLVGIKSREIYECPGAVTLLTAHKEIEDLTLVREVAHFKPIIENELSNLIYNALWFSSATQA
LIAYIKETQKVVNGTAKVKLYKGSAQVVARKSPSSLYDENLATYTSADTFDQDAAVGFIKLWGLPTKVHSEVQKSAK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=404, Percent_Identity=42.5742574257426, Blast_Score=303, Evalue=1e-82,
Organism=Homo sapiens, GI16950633, Length=404, Percent_Identity=42.5742574257426, Blast_Score=303, Evalue=1e-82,
Organism=Escherichia coli, GI1789563, Length=341, Percent_Identity=28.1524926686217, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324514, Length=413, Percent_Identity=37.046004842615, Blast_Score=264, Evalue=2e-71,
Organism=Drosophila melanogaster, GI21358151, Length=405, Percent_Identity=40, Blast_Score=282, Evalue=2e-76,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_STRP2 (Q04MW7)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_815608.1
- ProteinModelPortal:   Q04MW7
- SMR:   Q04MW7
- STRING:   Q04MW7
- EnsemblBacteria:   EBSTRT00000020352
- GeneID:   4441249
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0110
- eggNOG:   COG0137
- GeneTree:   EBGT00050000027547
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- ProtClustDB:   PRK00509
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44065; Mature: 43934

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 85-85 BINDING 115-115 BINDING 117-117 BINDING 121-121 BINDING 121-121 BINDING 122-122 BINDING 125-125 BINDING 173-173 BINDING 258-258 BINDING 270-270

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEKVILAYSGGLDTSVAITWLKKDYDVVAVCMDVGEGKDLDFIHDKALKVGAVESYVI
CCCCCEEEEECCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHEECCEEEEEE
DVKDEFATDYVLVAHQSHAYYEQKYPLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKG
ECCHHHCCCEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCC
NDQVRFEVSIAALDLNLKVIAPVREWKWSREEEIYYAKENGVPVPADLDNPYSVDQNLWG
CCEEEEEEEEEEEEEEEEEEECHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHCC
RANECGILENPWNQAPEEAFGITTSPEQAPDMPEYIEIEFSEGVPVSLNGEVLKLADLIQ
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEECCCCCEECCCCHHHHHHHHH
KLNEIAGKHGVGRIDHVENRLVGIKSREIYECPGAVTLLTAHKEIEDLTLVREVAHFKPI
HHHHHHCCCCCCCHHHHHHHEEECCCCCEEECCCCEEEEECHHHHHHHHHHHHHHHHHHH
IENELSNLIYNALWFSSATQALIAYIKETQKVVNGTAKVKLYKGSAQVVARKSPSSLYDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEEECCCCHHHHC
NLATYTSADTFDQDAAVGFIKLWGLPTKVHSEVQKSAK
CCCEECCCCCCCCCHHEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SKEKVILAYSGGLDTSVAITWLKKDYDVVAVCMDVGEGKDLDFIHDKALKVGAVESYVI
CCCCEEEEECCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHEECCEEEEEE
DVKDEFATDYVLVAHQSHAYYEQKYPLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKG
ECCHHHCCCEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCC
NDQVRFEVSIAALDLNLKVIAPVREWKWSREEEIYYAKENGVPVPADLDNPYSVDQNLWG
CCEEEEEEEEEEEEEEEEEEECHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHCC
RANECGILENPWNQAPEEAFGITTSPEQAPDMPEYIEIEFSEGVPVSLNGEVLKLADLIQ
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEECCCCCEECCCCHHHHHHHHH
KLNEIAGKHGVGRIDHVENRLVGIKSREIYECPGAVTLLTAHKEIEDLTLVREVAHFKPI
HHHHHHCCCCCCCHHHHHHHEEECCCCCEEECCCCEEEEECHHHHHHHHHHHHHHHHHHH
IENELSNLIYNALWFSSATQALIAYIKETQKVVNGTAKVKLYKGSAQVVARKSPSSLYDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEEECCCCHHHHC
NLATYTSADTFDQDAAVGFIKLWGLPTKVHSEVQKSAK
CCCEECCCCCCCCCHHEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA