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The map label for this gene is rfaI [H]

Identifier: 116515588

GI number: 116515588

Start: 401704

End: 402189

Strand: Direct

Name: rfaI [H]

Synonym: SPD_0400

Alternate gene names: 116515588

Gene position: 401704-402189 (Clockwise)

Preceding gene: 116516944

Following gene: 116515847

Centisome position: 19.63

GC content: 34.98

Gene sequence:

>486_bases
GTGAAAGAAGAGAAAAAAGCGATTGTCTTAGGTGCAGATAATGCTTATATGGATAAAGTTGAAACAACAATAAAATCTCT
TTGTGTTCATCATTATAATCTTAAGTTTTATGTTTTTAATGATGACCTTCCGCGAGAATGGTTCCAATTGATGGAAAAAA
GACTAGAGACTTTGAATAGTGAGATTGTGAATGTTCAGATTGACAGTAGTATTCTGAAAGGTTATCGTCTCCCCTTTGAG
GGACTTTCATATGCGGCTTTTTTTAGGTATTTTATTCCAAAGTATGTAAGCGAGTCTCGTGTACTGTATTTGGATTCAGA
TATTGTTGTAAGAAAATCGATAGATGAGCTGTGGGATTTGGACTTGACGGCTATACCCTTAGCTGCAGTTAGAGATGATT
TTTATACACATAATTTTAATTCTGGTGTTCTGTTAATCAACAATGGCATGTGGAGAGCAGAAAATGTCACGCAAGACCTG
ATTTAA

Upstream 100 bases:

>100_bases
CCACTGGTATGGTTTTACGAACGAATAGGCGAAAATAATGGTTACGTAGAAACATATGATGGGAATAATATGATGGGAAT
AAAAAGAAATAAGATAAAGA

Downstream 100 bases:

>100_bases
GACACAAATAAAAAATCTTCCAATTCCCTTGCAAAAAACAAGAAAGTGTGATAACATAGTACGGTATGTGGTGCTAGCAC
ATCCGCTATATTAGATCTAA

Product: glycosyl transferase family protein 8

Products: NA

Alternate protein names: Lipopolysaccharide 3-alpha-galactosyltransferase [H]

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLPREWFQLMEKRLETLNSEIVNVQIDSSILKGYRLPFE
GLSYAAFFRYFIPKYVSESRVLYLDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLINNGMWRAENVTQDL
I

Sequences:

>Translated_161_residues
MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLPREWFQLMEKRLETLNSEIVNVQIDSSILKGYRLPFE
GLSYAAFFRYFIPKYVSESRVLYLDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLINNGMWRAENVTQDL
I
>Mature_161_residues
MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLPREWFQLMEKRLETLNSEIVNVQIDSSILKGYRLPFE
GLSYAAFFRYFIPKYVSESRVLYLDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLINNGMWRAENVTQDL
I

Specific function: Adds the galactose(I) group on the glucose(I) group of LPS [H]

COG id: COG1442

COG function: function code M; Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 8 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002495
- InterPro:   IPR013645 [H]

Pfam domain/function: PF01501 Glyco_transf_8; PF08437 Glyco_transf_8N [H]

EC number: =2.4.1.44 [H]

Molecular weight: Translated: 18857; Mature: 18857

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLPREWFQLMEKRLETLNS
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHCH
EIVNVQIDSSILKGYRLPFEGLSYAAFFRYFIPKYVSESRVLYLDSDIVVRKSIDELWDL
HEEEEEECHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCC
DLTAIPLAAVRDDFYTHNFNSGVLLINNGMWRAENVTQDLI
CCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHCC
>Mature Secondary Structure
MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLPREWFQLMEKRLETLNS
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHCH
EIVNVQIDSSILKGYRLPFEGLSYAAFFRYFIPKYVSESRVLYLDSDIVVRKSIDELWDL
HEEEEEECHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCC
DLTAIPLAAVRDDFYTHNFNSGVLLINNGMWRAENVTQDLI
CCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2235496; 9535865; 11677609 [H]