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The map label for this gene is pfl [H]

Identifier: 116515577

GI number: 116515577

Start: 222584

End: 225022

Strand: Direct

Name: pfl [H]

Synonym: SPD_0235

Alternate gene names: 116515577

Gene position: 222584-225022 (Clockwise)

Preceding gene: 116516696

Following gene: 116516937

Centisome position: 10.88

GC content: 36.29

Gene sequence:

>2439_bases
ATGGTAAATACAGAAGTAGCAAGAACAACAATCAAGACAGAATATTTTGGCAGCCTTACTGAAAGGATGAACAAATATCG
AGAAGATGTTTTAAATAAAAAACCTTATATTGATGCTGAGAGAGCAGTTCTAGCAACACGCGCCTATGAACGATACAAGG
AACAACCTAATGTCCTAAAACGTGCATATATGCTGAAAGAAATTTTGGAAAATATGTCTATCTATATTGAAGAAGAATCT
ATGATTGCGGGAAATCAAGCTTCTTCCAATAAAGATGCTCCTATTTTTCCGGAATATACGCTAGAATTTGTTCTCAATGA
GTTGGATCTTTTTGAAAAGCGTGATGGAGATGTTTTCTATATTACAGAAGAAACAAAAGAACAACTTAGAAGTATTGCTC
CGTTTTGGGAAAATAATAATTTACGTGCTAGAGCTGGTGCCTTATTACCTGAAGAAGTGTCTGTTTATATGGAAACAGGA
TTCTTCGGTATGGAAGGTAAGATGAATTCTGGAGATGCTCACTTAGCAGTTAACTATCAGAAACTTTTGCAATTTGGTTT
AAGAGGTTTTGAAGAGCGGGCTCGTAAAGCAAAAGTAGCTCTAGATTTAACAGATCCAGCAAGTATTGATAAATATCATT
TTTACGACTCTATATTTATCGTAATCGATGCTATTAAAGTATATGCAAAGCGCTTTGTTGCTCTTGCTAAAAGTTTAGCC
GAAAATGCAAATCCTAAACGTAAGAAAGAATTACTTGAGATTGCAGATATTTGCTCTAGAGTCCCATATGAACCGGCAAC
TACTTTTGCAGAAGCTATTCAATCAGTTTGGTTTATTCAATGTATTTTACAAATTGAATCTAATGGCCACTCTCTTTCAT
ATGGCCGTTTTGATCAATATATGTATCCATATATGAAGGCTGATTTAGAAAGTGGTAAAGAAACAGAAGATAGCATTGTT
GAACGTCTGACAAATCTTTGGATTAAGACAATTACAATCAATAAGGTTCGCAGTCAATCACATACATTTTCTTCAGCAGG
AAGTCCTTTATATCAAAATGTTACAATTGGTGGACAGACTCGAGATAAGAAGGATGCTGTTAACCCATTATCTTATTTGG
TATTAAAATCAGTTGCACAAACCCATCTACCGCAACCTAATCTAACTGTACGTTACCATGCAGGTTTAGATGCTCGTTTC
ATGAATGAGTGTATTGAAGTGATGAAACTTGGTTTTGGTATGCCTGCATTTAATAATGATGAGATTATTATTCCTTCTTT
TATTGCAAAAGGAGTATTGGAAGATGATGCTTATGATTACAGTGCCATTGGATGTGTTGAAACGGCAGTTCCAGGGAAAT
GGGGCTATCGTTGCACAGGTATGAGTTATATGAACTTCCCTAAGGTTCTACTTATCACGATGAATGATGGAATTGATCCG
GCTTCGGGTAAACGGTTTGCACCAAGCTTTGGTCATTTTAAGGATATGAAGAACTTTTCTGAATTAGAAAATGCTTGGGA
TAAAACACTAAGATATTTGACACGAATGAGTGTTATTGTTGAAAATTCTATTGATTTATCATTGGAACGAGAAGTTCCTG
ATATTCTATGTTCAGCATTGACTGATGATTGTATTGGTCGTGGAAAACACCTTAAAGAAGGTGGAGCAGTATATGATTAT
ATATCAGGATTGCAAGTTGGAATTGCAAATTTGTCGGATTCATTAGCTGCAATTAAAAAATTGGTGTTTGAGGAAGAACG
TATAAGCCCAAGTCAGCTTTGGCATGCACTGGAAACAGATTATGCCGGAGAAGAAGGTAAGGTCATTCAAGAAATGTTGA
TTCATGATGCACCTAAGTATGGTAATGATGATGATTATGCTGACAAATTGGTTACTGCTGCTTATGACATTTATGTTGAT
GAAATTGCTAAATATCCTAATACACGTTATGGAAGAGGGCCTATTGGAGGAATTCGTTATTCAGGAACATCTTCTATATC
AGCCAACGTAGGGCAGGGACGTGGAACATTAGCAACTCCAGATGGACGCAACGCGGGTACACCGTTAGCAGAGGGTTGTT
CACCATCACATAATATGGATCAACACGGCCCTACATCTGTTTTAAAATCTGTTTCAAAATTACCAACAGATGAAATCGTA
GGTGGGGTTCTCTTAAATCAGAAAGTAAATCCTCAAACGTTAGCCAAAGAAGAAGATAAATTAAAACTAATTGCTTTGTT
ACGAACATTCTTTAATCGTTTACATGGGTACCATATTCAATACAATGTTGTTTCCAGAGAGACGTTGATTGACGCTCAGA
AACATCCTGAAAAACACAGAGACTTAATTGTTCGTGTTGCAGGATACTCTGCATTCTTCAATGTTCTTTCTAAGGCAACC
CAAGATGACATTATAGGACGTACTGAGCATACTTTGTAA

Upstream 100 bases:

>100_bases
GAGTTAAAATTTTTCTAGTTAAAAGCTTGAAAATTTCTATAAAAATCGGTATTATATTTTCGAAAGAAATGAAAATATTT
TCGAAAGAAAGGTGCTTACG

Downstream 100 bases:

>100_bases
AATAAAGAGGTTCTTTTTATGGAATTTATGCTTGACACATTAAATTTAGATGAGATTAAAAAGTGGTCTGAAATTTTGCC
GCTAGCTGGGGTAACTTCAA

Product: pyruvate formate-lyase

Products: NA

Alternate protein names: Pyruvate formate-lyase 3 [H]

Number of amino acids: Translated: 812; Mature: 812

Protein sequence:

>812_residues
MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVLATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEES
MIAGNQASSNKDAPIFPEYTLEFVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSIFIVIDAIKVYAKRFVALAKSLA
ENANPKRKKELLEIADICSRVPYEPATTFAEAIQSVWFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIV
ERLTNLWIKTITINKVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRYHAGLDARF
MNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAVPGKWGYRCTGMSYMNFPKVLLITMNDGIDP
ASGKRFAPSFGHFKDMKNFSELENAWDKTLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDY
ISGLQVGIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDDDYADKLVTAAYDIYVD
EIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDGRNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIV
GGVLLNQKVNPQTLAKEEDKLKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKAT
QDDIIGRTEHTL

Sequences:

>Translated_812_residues
MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVLATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEES
MIAGNQASSNKDAPIFPEYTLEFVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSIFIVIDAIKVYAKRFVALAKSLA
ENANPKRKKELLEIADICSRVPYEPATTFAEAIQSVWFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIV
ERLTNLWIKTITINKVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRYHAGLDARF
MNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAVPGKWGYRCTGMSYMNFPKVLLITMNDGIDP
ASGKRFAPSFGHFKDMKNFSELENAWDKTLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDY
ISGLQVGIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDDDYADKLVTAAYDIYVD
EIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDGRNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIV
GGVLLNQKVNPQTLAKEEDKLKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKAT
QDDIIGRTEHTL
>Mature_812_residues
MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVLATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEES
MIAGNQASSNKDAPIFPEYTLEFVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSIFIVIDAIKVYAKRFVALAKSLA
ENANPKRKKELLEIADICSRVPYEPATTFAEAIQSVWFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIV
ERLTNLWIKTITINKVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRYHAGLDARF
MNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAVPGKWGYRCTGMSYMNFPKVLLITMNDGIDP
ASGKRFAPSFGHFKDMKNFSELENAWDKTLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDY
ISGLQVGIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDDDYADKLVTAAYDIYVD
EIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDGRNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIV
GGVLLNQKVNPQTLAKEEDKLKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKAT
QDDIIGRTEHTL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787044, Length=815, Percent_Identity=50.5521472392638, Blast_Score=820, Evalue=0.0,
Organism=Escherichia coli, GI1790388, Length=798, Percent_Identity=36.7167919799499, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI1787131, Length=581, Percent_Identity=27.1944922547332, Blast_Score=191, Evalue=2e-49,
Organism=Escherichia coli, GI48994926, Length=594, Percent_Identity=26.4309764309764, Blast_Score=185, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 91341; Mature: 91341

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVLATRAYERYKEQPNVLK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHH
RAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLEFVLNELDLFEKRDGDVFY
HHHHHHHHHHCCEEEEECCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEE
ITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETGFFGMEGKMNSGDAHLAVNYQ
EEHHHHHHHHHHCCCCCCCCCEEHHCCCCHHHHHHHHHCCEEECCCCCCCCCCEEEECHH
KLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSIFIVIDAIKVYAKRFVALAKSLA
HHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENANPKRKKELLEIADICSRVPYEPATTFAEAIQSVWFIQCILQIESNGHSLSYGRFDQY
HCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
MYPYMKADLESGKETEDSIVERLTNLWIKTITINKVRSQSHTFSSAGSPLYQNVTIGGQT
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEHHHHHCCCCCHHCCCCCCEEEEEECCCC
RDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRYHAGLDARFMNECIEVMKLGFGMPAFNND
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCC
EIIIPSFIAKGVLEDDAYDYSAIGCVETAVPGKWGYRCTGMSYMNFPKVLLITMNDGIDP
CEEEHHHHHCCHHCCCCCCCHHHHHHHHCCCCCCCCEECCCCCCCCCEEEEEEECCCCCC
ASGKRFAPSFGHFKDMKNFSELENAWDKTLRYLTRMSVIVENSIDLSLEREVPDILCSAL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHH
TDDCIGRGKHLKEGGAVYDYISGLQVGIANLSDSLAAIKKLVFEEERISPSQLWHALETD
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
YAGEEGKVIQEMLIHDAPKYGNDDDYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRY
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
SGTSSISANVGQGRGTLATPDGRNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHH
GGVLLNQKVNPQTLAKEEDKLKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHR
HHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEEEECHHHHHCCHHCCHHHH
DLIVRVAGYSAFFNVLSKATQDDIIGRTEHTL
CEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVLATRAYERYKEQPNVLK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHH
RAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLEFVLNELDLFEKRDGDVFY
HHHHHHHHHHCCEEEEECCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEE
ITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETGFFGMEGKMNSGDAHLAVNYQ
EEHHHHHHHHHHCCCCCCCCCEEHHCCCCHHHHHHHHHCCEEECCCCCCCCCCEEEECHH
KLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSIFIVIDAIKVYAKRFVALAKSLA
HHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENANPKRKKELLEIADICSRVPYEPATTFAEAIQSVWFIQCILQIESNGHSLSYGRFDQY
HCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
MYPYMKADLESGKETEDSIVERLTNLWIKTITINKVRSQSHTFSSAGSPLYQNVTIGGQT
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEHHHHHCCCCCHHCCCCCCEEEEEECCCC
RDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRYHAGLDARFMNECIEVMKLGFGMPAFNND
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCC
EIIIPSFIAKGVLEDDAYDYSAIGCVETAVPGKWGYRCTGMSYMNFPKVLLITMNDGIDP
CEEEHHHHHCCHHCCCCCCCHHHHHHHHCCCCCCCCEECCCCCCCCCEEEEEEECCCCCC
ASGKRFAPSFGHFKDMKNFSELENAWDKTLRYLTRMSVIVENSIDLSLEREVPDILCSAL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHH
TDDCIGRGKHLKEGGAVYDYISGLQVGIANLSDSLAAIKKLVFEEERISPSQLWHALETD
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
YAGEEGKVIQEMLIHDAPKYGNDDDYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRY
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
SGTSSISANVGQGRGTLATPDGRNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHH
GGVLLNQKVNPQTLAKEEDKLKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHR
HHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEEEECHHHHHCCHHCCHHHH
DLIVRVAGYSAFFNVLSKATQDDIIGRTEHTL
CEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]