Click here to switch to the map view.

The map label for this gene is metK

Identifier: 116515567

GI number: 116515567

Start: 683650

End: 684840

Strand: Direct

Name: metK

Synonym: SPD_0664

Alternate gene names: 116515567

Gene position: 683650-684840 (Clockwise)

Preceding gene: 116516888

Following gene: 116516687

Centisome position: 33.41

GC content: 44.0

Gene sequence:

>1191_bases
ATGTCAGAACGTAAATTATTCACGTCTGAATCTGTATCTGAGGGGCATCCGGATAAGATTGCAGACCAAATTTCAGATGC
GATTTTGGATGCTATTTTAGCAAAGGATCCAGAGGCGCACGTTGCTGCTGAAACAGCTGTATATACTGGTTCTGTCCACG
TTTTTGGTGAAATTTCTACAAATGCCTATGTGGATATTAACCGTGTGGTTCGTGATACCATTGCAGAGATTGGTTATACC
AATACAGAATATGGATTTTCTGCTGAGACGGTGGGAGTACACCCATCTTTGGTGGAACAATCTCCTGACATCGCTCAAGG
TGTTAACGAAGCCTTGGAGGTTCGCGGAAATGCTGATCAAGATCCACTGGACTTGATTGGAGCAGGTGACCAAGGGCTCA
TGTTTGGATTTGCAGTAGATGAAACAGAAGAGCTTATGCCATTGCCAATTGCACTCAGTCATAAATTGGTTCGTCGTCTG
GCAGAACTTCGTAAGTCTGGAGAAATTAGCTATCTCCGTCCAGATGCAAAATCACAAGTTACAGTTGAGTACGATGAAAA
TGACCGTCCGGTACGTGTAGATACAGTCGTTATTTCTACTCAGCATGATCCAGAGGCCACTAATGAACAAATCCATCAAG
ATGTGATTGACAAGGTCATCAAAGAAGTTATTCCATCTTCTTATCTTGATGATAAGACAAAATTCTTTATCAATCCGACA
GGTCGTTTTGTAATCGGTGGTCCTCAAGGGGACTCAGGTTTGACTGGTCGTAAGATTATTGTAGATACTTATGGTGGCTA
CTCTCGTCATGGTGGTGGTGCCTTCTCTGGTAAAGATGCGACTAAGGTGGATCGTTCAGCCTCTTATGCGGCTCGCTATA
TTGCCAAGAATATCGTTGCAGCAGGCCTTGCTAAGAAGGCAGAAGTGCAGTTGGCCTATGCTATCGGTGTTGCGCAACCT
GTTTCTGTTCGTATCGATACTTTCGGTACAGGAACAGTAGCTGAAAGTCAACTTGAAAAAGCGGCTCGTCAAATCTTTGA
CCTTCGCCCTGCAGGGATTATCCAAATGCTGGACCTCAAGCGTCCAATTTACCGTCAAACATCGGCTTACGGTCACATGG
GACGTACAGATATTGATCTTCCATGGGAACGTTTGGATAAGGTAGATGCTTTGAAAGAAGCAGTAAAATAA

Upstream 100 bases:

>100_bases
GTTATATAGTCCCGATAAGATGGTAGGTATTTATTACGAAGAGTTTTCCTATCAGTACTTTGTAACTCTATAACAATATT
TTTTAAGGGGGGACATTTTT

Downstream 100 bases:

>100_bases
GATTTTAAGAGGGGAACGTCCTCTCTTTTTTATAGTTTTTAACTATACTGGGATACTGTTCTGAAAATCCATTTTGCGAA
AGTAGAGATTTACATGTATA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAETAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYT
NTEYGFSAETVGVHPSLVEQSPDIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDKVIKEVIPSSYLDDKTKFFINPT
GRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSGKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQP
VSVRIDTFGTGTVAESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALKEAVK

Sequences:

>Translated_396_residues
MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAETAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYT
NTEYGFSAETVGVHPSLVEQSPDIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDKVIKEVIPSSYLDDKTKFFINPT
GRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSGKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQP
VSVRIDTFGTGTVAESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALKEAVK
>Mature_395_residues
SERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAETAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTN
TEYGFSAETVGVHPSLVEQSPDIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRLA
ELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDKVIKEVIPSSYLDDKTKFFINPTG
RFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSGKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPV
SVRIDTFGTGTVAESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALKEAVK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=381, Percent_Identity=55.9055118110236, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI5174529, Length=382, Percent_Identity=56.020942408377, Blast_Score=405, Evalue=1e-113,
Organism=Escherichia coli, GI1789311, Length=391, Percent_Identity=59.5907928388747, Blast_Score=477, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17538494, Length=388, Percent_Identity=55.6701030927835, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17538498, Length=388, Percent_Identity=55.6701030927835, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25145633, Length=392, Percent_Identity=55.3571428571429, Blast_Score=408, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17551082, Length=387, Percent_Identity=54.0051679586563, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI32565905, Length=388, Percent_Identity=51.5463917525773, Blast_Score=358, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI32565911, Length=336, Percent_Identity=53.5714285714286, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI25145635, Length=340, Percent_Identity=52.9411764705882, Blast_Score=339, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI32565903, Length=310, Percent_Identity=54.5161290322581, Blast_Score=319, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI32565909, Length=137, Percent_Identity=40.1459854014599, Blast_Score=101, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6323209, Length=387, Percent_Identity=55.0387596899225, Blast_Score=411, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6320710, Length=394, Percent_Identity=53.2994923857868, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45552159, Length=381, Percent_Identity=53.2808398950131, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580529, Length=381, Percent_Identity=53.2808398950131, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580537, Length=381, Percent_Identity=53.2808398950131, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580533, Length=381, Percent_Identity=53.2808398950131, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580535, Length=381, Percent_Identity=53.2808398950131, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580531, Length=381, Percent_Identity=53.2808398950131, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580527, Length=381, Percent_Identity=52.755905511811, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580525, Length=381, Percent_Identity=52.755905511811, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580541, Length=383, Percent_Identity=50.3916449086162, Blast_Score=346, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24580539, Length=126, Percent_Identity=50, Blast_Score=118, Evalue=8e-27,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_STRP2 (Q04LE0)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816158.1
- ProteinModelPortal:   Q04LE0
- SMR:   Q04LE0
- STRING:   Q04LE0
- PhosSite:   Q04LE0
- EnsemblBacteria:   EBSTRT00000019616
- GeneID:   4442877
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0664
- eggNOG:   COG0192
- GeneTree:   EBGT00050000027507
- HOGENOM:   HBG443662
- OMA:   QDEHARV
- ProtClustDB:   PRK05250
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 43116; Mature: 42985

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAETAVYTGSVHVFGEIST
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEECCEEEEEEECC
NAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSPDIAQGVNEALEVRGNADQ
CEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCC
DPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRLAELRKSGEISYLRPDAKSQV
CCHHEECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCEE
TVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDKVIKEVIPSSYLDDKTKFFINPT
EEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCC
GRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSGKDATKVDRSASYAARYIAKNIVA
CCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVAESQLEKAARQIFDLRPAGIIQMLDLK
HHCCHHHHEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC
RPIYRQTSAYGHMGRTDIDLPWERLDKVDALKEAVK
CHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAETAVYTGSVHVFGEIST
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEECCEEEEEEECC
NAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSPDIAQGVNEALEVRGNADQ
CEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCC
DPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRLAELRKSGEISYLRPDAKSQV
CCHHEECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCEE
TVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDKVIKEVIPSSYLDDKTKFFINPT
EEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCC
GRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSGKDATKVDRSASYAARYIAKNIVA
CCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVAESQLEKAARQIFDLRPAGIIQMLDLK
HHCCHHHHEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC
RPIYRQTSAYGHMGRTDIDLPWERLDKVDALKEAVK
CHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA