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The map label for this gene is hsdS [H]

Identifier: 116515523

GI number: 116515523

Start: 460865

End: 462433

Strand: Reverse

Name: hsdS [H]

Synonym: SPD_0453

Alternate gene names: 116515523

Gene position: 462433-460865 (Counterclockwise)

Preceding gene: 116516554

Following gene: 116515761

Centisome position: 22.6

GC content: 33.59

Gene sequence:

>1569_bases
ATGACACCAGAACAACTTAAAGCAAGTATTCTCCAAAGAGCGATGGAAGGGAAATTAGTGCCGCAAAATCCCAATGACGA
ACCTGCAAGTGAATTATTAAAGAGAATTAAAGCTGAAAAAGAAAAACTTATCAGTGAAGGAAAAATCAAACGAGATAAAA
AGGAAACTGAGATATTTCGTGGTGATGATGGGAAACATTATGGGAAGTTTGCTGATGGAAGTACTCAAGAAATTGATGTT
CCTTATGATATTCCTGATACTTGGGAGTGGGTGAGGTTTTCTACATTGGTTGAAATTGTCAGAGGTGGCTCTCCACGACC
AATCAAAGATTATCTTACTTCTGAAGTAGATGGAATAAATTGGATAAAAATAGGTGATACTGAAAAGGGTGAAAAGTATA
TAAATAATGTTAAAGAAAAAATCAAAAAATCAGGGCTTAACAAAACTAGATTTGTAAAAAAAGGTACATTTTTGTTAACT
AATTCTATGAGTTTTGGTAGACCTTATATTTTGAATGTTGATGGTGCAATACACGATGGATGGTTGGCTATTTCGAACTA
TGAAAACTCATTAAATAAAGATTACCTATTCTATATTCTTTCATCAAATGTAGTTTATTCTCAATTTCTATCTCTAATTA
GTGGAGCTGTTGTGAAAAACTTGAATAGTGATAAAGTTGCTTCTATTCTTATCCCTCTCCCCCCACTATCCGAACAACAA
CGCATAATAGAAGCAATCGAATCAGCTTTAGAAAAAGTAGATGAATATGCTGAAAGTTATAATAGACTAGAACAGCTAGA
TAAAGAATTTCCAGATAAACTAAAAAAATCTATTCTTCAATATGCTATGCAAGGAAAATTAGTTGAACAAGACCCAAATG
ATGAATCAGTCGAAGTTTTACTTGAAAAAATACGAGCAGAAAAACAAAAACTATTTGAAGAAGGCAAGATTAAAAAGAAA
GATTTGGACATTTCTATTGTTTCCCAAGGAGATGATAACTCTTATTATGAGGAAGTACCTTGTGAAATACCAGAAAGTTG
GGAATGGGTGAGGTTAAACGATATAACCTCCTACATTCAAAGAGGGAAGTCTCCAAAATATTCAAATATTCCTATATACC
CTGTGATTGCTCAGAAATGTAATCAATGGTCTGGCTTTTCTATTGACTTAGCAAGATTTATTGATCCCGAAACAGTTCAT
AGTTACCAGAAAGAAAGATTACTAAGGGATGGTGATTTAATGTGGAATTCAACAGGTCTAGGTACGTTAGGACGATTAGC
TATTTACCATGAAAATAAAAATCCTTATGCCTGGGCTGTTGCAGATAGCCATGTTACAGTTATAAGAGTCTTATCGGGGG
TTATTAATTGCCATTTCATCTATAATTTTCTTTCCTCTCCTATTGTTCAGTCAGTTATTGAAGAAAAAGCGTCGGGTAGT
ACAAAACAAAAAGAATTATTAACGAAAACTATAAAAGAATATTTAATCCCCCTCCCACCCCTTCCTGAACAATCCAGAAT
CGTTGATAAAATCGAACAATTTTTCGCCCATATTGACGCACTAATTTAG

Upstream 100 bases:

>100_bases
CCTTTGAGTTGATTCAGAATTATCAAGCGGAAAGAGCAACTTTAAATCATAAGATTGATAATGTATTAGCTGATATTTTG
CAGTTGTTGGAGGACAAATA

Downstream 100 bases:

>100_bases
TATAATAGTTTTACGTGCGGTGGAATTTCTATCAAACATAAGATAGGAGTTTTACGTTGAAAGATTTTGAATTACATTTG
AAGAAAGCTGGTTTAGCTGA

Product: type I restriction-modification system, S subunit

Products: NA

Alternate protein names: S.EcoAI; Type I restriction enzyme EcoAI specificity protein; S protein [H]

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLISEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDV
PYDIPDTWEWVRFSTLVEIVRGGSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLSEQQ
RIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKK
DLDISIVSQGDDNSYYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVH
SYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYAWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGS
TKQKELLTKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI

Sequences:

>Translated_522_residues
MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLISEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDV
PYDIPDTWEWVRFSTLVEIVRGGSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLSEQQ
RIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKK
DLDISIVSQGDDNSYYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVH
SYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYAWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGS
TKQKELLTKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI
>Mature_521_residues
TPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLISEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVP
YDIPDTWEWVRFSTLVEIVRGGSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLTN
SMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLSEQQR
IIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKD
LDISIVSQGDDNSYYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHS
YQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYAWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGST
KQKELLTKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 59634; Mature: 59502

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLISEGKIKRDKKETEIFR
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHEE
GDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRGGSPRPIKDYLTSEVDGIN
CCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCE
WIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLTNSMSFGRPYILNVDGAIHDG
EEEECCCCCHHHHHHHHHHHHHHCCCCHHHEEECCCEEEECCCCCCCCEEEECCCCCCCC
WLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLSEQQ
EEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHH
RIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYYEEVPCEIPESWEWVRLNDITSYIQ
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHCCCCCCCCCCEEEHHHHHHHHH
RGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGL
CCCCCCCCCCCCHHHHHHHCCCCCCCCEEHHHHCCHHHHHHHHHHHHHHCCCEEECCCCC
GTLGRLAIYHENKNPYAWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGS
CCHHEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TKQKELLTKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLISEGKIKRDKKETEIFR
CHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHEE
GDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRGGSPRPIKDYLTSEVDGIN
CCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCE
WIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLTNSMSFGRPYILNVDGAIHDG
EEEECCCCCHHHHHHHHHHHHHHCCCCHHHEEECCCEEEECCCCCCCCEEEECCCCCCCC
WLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLSEQQ
EEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHH
RIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYYEEVPCEIPESWEWVRLNDITSYIQ
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHCCCCCCCCCCEEEHHHHHHHHH
RGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGL
CCCCCCCCCCCCHHHHHHHCCCCCCCCEEHHHHCCHHHHHHHHHHHHHHCCCEEECCCCC
GTLGRLAIYHENKNPYAWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGS
CCHHEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TKQKELLTKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2642743 [H]