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The map label for this gene is nudF [H]

Identifier: 116515368

GI number: 116515368

Start: 889286

End: 889831

Strand: Direct

Name: nudF [H]

Synonym: SPD_0875

Alternate gene names: 116515368

Gene position: 889286-889831 (Clockwise)

Preceding gene: 116516172

Following gene: 116516438

Centisome position: 43.46

GC content: 41.39

Gene sequence:

>546_bases
ATGGAGTTTGAAGAAAAAACGCTTAGCCGAAAAGAAATCTATCAAGGACCAATATTTAAACTGGTCCAAGATCAGGTTGA
ATTACCAGAAGGCAAGGGAACTGCCCGACGGGATTTGATTTTCCACAATGGGGCTGTCTGTGTTTTAGCAGTAACGGATG
AACAAAAACTTATCTTGGTCAAGCAGTACCGCAAAGCTATCGAGGCTGTCTCTTACGAAATTCCAGCCGGAAAATTGGAA
GTAGGAGAAAACACAGCCCCTGTGGCAGCTGCCCTTCGTGAATTAGAGGAAGAAACAGCCTATACAGGGAAATTAGAACT
CTTGTACGATTTTTATTCAGCTATTGGCTTTTGTAATGAGAAGTTAAAACTATATTTAGCAAGCGATTTGACAAAAGTGG
AAAATCCGCGTCCGCAGGATGAGGATGAAACCTTGGAAGTCCTTGAAGTGAGCTTAGAAGAAGCGAAAGAATTAATCCAA
TCAGGTCATATCTGTGATGCCAAGACAATTATGGCTGTTCAGTATTGGGAGTTGCAGAAAAAATAG

Upstream 100 bases:

>100_bases
GCCAGCAGATGCTATTGCTATTGGTCGCGGTCGTCAGATCAATAAAGACGAATATGCAACACGTCTTCCTCATCATCCTA
AGAACCAGTAGGAGCCTATC

Downstream 100 bases:

>100_bases
AGGAGGTCAGTATGGGTAAATCTTTATTAACGGATGAAATGATTGAAAGAGCTAATAGAGGCGAAAAAATTTCAGGTCCT
CCTTTGCTAGATGATAATGA

Product: hydrolase, NUDIX family protein

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNGAVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLE
VGENTAPVAAALRELEEETAYTGKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
SGHICDAKTIMAVQYWELQKK

Sequences:

>Translated_181_residues
MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNGAVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLE
VGENTAPVAAALRELEEETAYTGKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
SGHICDAKTIMAVQYWELQKK
>Mature_181_residues
MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNGAVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLE
VGENTAPVAAALRELEEETAYTGKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
SGHICDAKTIMAVQYWELQKK

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6319587, Length=150, Percent_Identity=33.3333333333333, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 20543; Mature: 20543

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNGAVCVLAVTDEQKLILV
CCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHEEEECCCEEEEEECCCCCCHHH
KQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYTGKLELLYDFYSAIGFCNE
HHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCH
KLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQSGHICDAKTIMAVQYWELQK
HHEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
K
C
>Mature Secondary Structure
MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNGAVCVLAVTDEQKLILV
CCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHEEEECCCEEEEEECCCCCCHHH
KQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYTGKLELLYDFYSAIGFCNE
HHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCH
KLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQSGHICDAKTIMAVQYWELQK
HHEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10542272 [H]