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The map label for this gene is dapF [H]

Identifier: 116515294

GI number: 116515294

Start: 400393

End: 401238

Strand: Reverse

Name: dapF [H]

Synonym: BCc_380

Alternate gene names: 116515294

Gene position: 401238-400393 (Counterclockwise)

Preceding gene: 116515296

Following gene: 116515293

Centisome position: 96.36

GC content: 21.99

Gene sequence:

>846_bases
ATGTTATTAAGAAAAAAATTTATTTTTTTTTTTAAAATGCATGGATTAGGTAATGATTTTGTTTTAATTTATTCTAAAAA
ATATCTTTTATTTTCTTCAGAATTAATAAAAAAATGGTCAAATAGATTTTTAGGTATTGGTTTTGATCAATTATTATTAA
TTCAAAAAAAAAAGAATAATAAAGCTCAGTTTTATTATAGAATATTTAATTCAAATGGAATAGAAGTAGAACAATGTGGT
AATGGAGCAAGATGTGTTGCATATTATTTGTTTTTAAAAAAAAAAATTAAAGAAAAAAAAATTTGTGTGAGTACAAATAA
TAGATATTTATTTTTAGAACATATTAAAAAAAATATTTTTAAAGTTGATATGGGTACGCCTATTTTTACTCCTAAAAATA
TTCCTTTTATAACAAAGACTGAAAAATTTTTTTATTCTATTATTTTTAATAAAAAAAAATATTTTTTTAGTGTTGTAACA
TTAGGTAATCCACACTGTGTTATTCAAGTTAATAATCTAAAAAATTTCCCAGTAAAAAAAGTGGGTTCTTATATTTCGAA
TAACATTTTATTTCCAGAAGGCGTGAATGTTGGTTTTATGCAGATTATTTCAAAAAAAAAAATTTTATTGCGTGTATATG
AAAGACATGTAGGAGAAACTAATGCATGTGGAAGCGGAGCATGTGCTGCGGTTGCTATAGGTATACGAAATAAATTATTA
TCTAATCATGTAGTAGTTAAATTATTATATGGAAAATTAAAAATTTTTTGGAATGGAATATTAGGAGATACATTATATAT
GTCAGGTAAAGCTTCACATGTATATGATGGTTATATTAAGTATTAA

Upstream 100 bases:

>100_bases
AAATAATAATGTATTATTCATAATAATATCCAATATAAATATTTTATAAACATTTTTAAAATTAAATTATATCATTTATT
TTTATATAAGGATATTTATT

Downstream 100 bases:

>100_bases
TTTTATTAAATTTAAAAATTTATTTAATTTTAATATAAAAAAAATATTTTATTTATATTTTTATATTAAAAATTTTTATT
AAAGATTTTCTGATATTAGT

Product: hypothetical protein

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MLLRKKFIFFFKMHGLGNDFVLIYSKKYLLFSSELIKKWSNRFLGIGFDQLLLIQKKKNNKAQFYYRIFNSNGIEVEQCG
NGARCVAYYLFLKKKIKEKKICVSTNNRYLFLEHIKKNIFKVDMGTPIFTPKNIPFITKTEKFFYSIIFNKKKYFFSVVT
LGNPHCVIQVNNLKNFPVKKVGSYISNNILFPEGVNVGFMQIISKKKILLRVYERHVGETNACGSGACAAVAIGIRNKLL
SNHVVVKLLYGKLKIFWNGILGDTLYMSGKASHVYDGYIKY

Sequences:

>Translated_281_residues
MLLRKKFIFFFKMHGLGNDFVLIYSKKYLLFSSELIKKWSNRFLGIGFDQLLLIQKKKNNKAQFYYRIFNSNGIEVEQCG
NGARCVAYYLFLKKKIKEKKICVSTNNRYLFLEHIKKNIFKVDMGTPIFTPKNIPFITKTEKFFYSIIFNKKKYFFSVVT
LGNPHCVIQVNNLKNFPVKKVGSYISNNILFPEGVNVGFMQIISKKKILLRVYERHVGETNACGSGACAAVAIGIRNKLL
SNHVVVKLLYGKLKIFWNGILGDTLYMSGKASHVYDGYIKY
>Mature_281_residues
MLLRKKFIFFFKMHGLGNDFVLIYSKKYLLFSSELIKKWSNRFLGIGFDQLLLIQKKKNNKAQFYYRIFNSNGIEVEQCG
NGARCVAYYLFLKKKIKEKKICVSTNNRYLFLEHIKKNIFKVDMGTPIFTPKNIPFITKTEKFFYSIIFNKKKYFFSVVT
LGNPHCVIQVNNLKNFPVKKVGSYISNNILFPEGVNVGFMQIISKKKILLRVYERHVGETNACGSGACAAVAIGIRNKLL
SNHVVVKLLYGKLKIFWNGILGDTLYMSGKASHVYDGYIKY

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=271, Percent_Identity=46.1254612546125, Blast_Score=266, Evalue=8e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 32511; Mature: 32511

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLRKKFIFFFKMHGLGNDFVLIYSKKYLLFSSELIKKWSNRFLGIGFDQLLLIQKKKNN
CCCHHEEEEEEEECCCCCCEEEEEECCEEEHHHHHHHHHHCCEECCCHHHEEEEEECCCC
KAQFYYRIFNSNGIEVEQCGNGARCVAYYLFLKKKIKEKKICVSTNNRYLFLEHIKKNIF
CEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEEEHHHHHCCEE
KVDMGTPIFTPKNIPFITKTEKFFYSIIFNKKKYFFSVVTLGNPHCVIQVNNLKNFPVKK
EEECCCCCCCCCCCCEEEEHHHHEEEEEECCCEEEEEEEEECCCEEEEEECCCCCCCHHH
VGSYISNNILFPEGVNVGFMQIISKKKILLRVYERHVGETNACGSGACAAVAIGIRNKLL
HHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEHHHHHHHH
SNHVVVKLLYGKLKIFWNGILGDTLYMSGKASHVYDGYIKY
CCHHEEEEHHHHEEEEECCCCCCEEEECCCCCEECCCEECC
>Mature Secondary Structure
MLLRKKFIFFFKMHGLGNDFVLIYSKKYLLFSSELIKKWSNRFLGIGFDQLLLIQKKKNN
CCCHHEEEEEEEECCCCCCEEEEEECCEEEHHHHHHHHHHCCEECCCHHHEEEEEECCCC
KAQFYYRIFNSNGIEVEQCGNGARCVAYYLFLKKKIKEKKICVSTNNRYLFLEHIKKNIF
CEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEEEHHHHHCCEE
KVDMGTPIFTPKNIPFITKTEKFFYSIIFNKKKYFFSVVTLGNPHCVIQVNNLKNFPVKK
EEECCCCCCCCCCCCEEEEHHHHEEEEEECCCEEEEEEEEECCCEEEEEECCCCCCCHHH
VGSYISNNILFPEGVNVGFMQIISKKKILLRVYERHVGETNACGSGACAAVAIGIRNKLL
HHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEHHHHHHHH
SNHVVVKLLYGKLKIFWNGILGDTLYMSGKASHVYDGYIKY
CCHHEEEEHHHHEEEEECCCCCCEEEECCCCCEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]