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The map label for this gene is tuf

Identifier: 116515261

GI number: 116515261

Start: 369501

End: 370685

Strand: Reverse

Name: tuf

Synonym: BCc_343

Alternate gene names: 116515261

Gene position: 370685-369501 (Counterclockwise)

Preceding gene: 116515262

Following gene: 116515260

Centisome position: 89.03

GC content: 31.9

Gene sequence:

>1185_bases
ATGTCAAAAGAAAAATTTAATCGTTCTAAACCACATATTAATGTCGGAACTATTGGACATGTAGATCATGGAAAAACTAC
TTTAACATCAGCTATTACTACAGTTTTATCAAAAAGATTTGGTGGTAAAGCATGTGCATTTGAACAAATTGATAATGCTC
CAGAAGAAAAAGCTAGAGGTATTACAATAAATACTTCACATGTTGAATATGATACTGAATTACGTCATTATGCTCATGTA
GATTGTCCTGGACATGCTGATTATATAAAAAATATGATTACTGGTGCTGCACAAATGGATGGAGCTATTCTAGTAGTAGC
TGCTACTGATGGTCCTATGCCTCAAACTAGAGAACATATTTTGTTAGGTAGACAAGTTGGTGTTCCTCATATAATTGTTT
TTTTAAATAAATGTGATATGGTTGATGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGATTTATTAACTCAA
TATGATTTTCCAGGAGATAATATTCCTATTATTCGTGGTTCAGCTTTAAAAGCATTAGAAGGTGAAAAAATTTGGGAAGA
TAAAATCATTGAATTAGCTAATTCTTTAGATAAATATATTCCTATTCCTGTAAGAGCTGTTGATGAACCATTTCTGCTTC
CAATTGAAGATGTTTTTTCTATATCTGGTCGTGGAACTGTAGTTACTGGACGTATTGAACGAGGTATATTAAAAGTTGGT
GAAGAAGTTGAAATTGTTGGTATAAAATCTACTACAAAAACAATATGTACTGGTGTAGAAATGTTTCGTAAATTATTAGA
TGAAGGTAGAGCTGGAGAAAATGTAGGAATTTTACTAAGAGGAACAAAACGAGAAGATATTGAGCGTGGACAAGTTCTTG
CAAAACCTGGAACTATTAATCCTCATGTAAAATTTGAATCTGAAGTTTATGTATTATCTAAAGAAGAAGGAGGTAGACAT
ACTCCATTTTTTAAAGGATATCGTCCTCAATTTTATTTTCGTACTACTGATGTTACAGGTTCAATTGAGCTTCCTGAAAA
TATAGAAATGGTTATGCCGGGTGATAATATTAATATGGTAGTTACATTAATTCATCCAATTGCTATGGCTGAAGGATTAC
GATTTGCAATTAGAGAAGGTGGAAGAACAGTAGGAGCTGGTGTAGTAACAAAAGTTATTGCATAA

Upstream 100 bases:

>100_bases
TAGAAGTATTTATGATAGTATTGTTTCTACACAATAAAATTTTTTTATAAAAATAAATATTTTAAATAATTAATATTATG
TATTAAATTAAGGAATAGTA

Downstream 100 bases:

>100_bases
TATAGATATAAATTTTATTATATATCAATATTTTAATATTTTGTCAAGATTAGAGGTTTTCTCTTTTCTTGACATAATTT
TTTTAATAATTAACTTATTA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSKEKFNRSKPHINVGTIGHVDHGKTTLTSAITTVLSKRFGGKACAFEQIDNAPEEKARGITINTSHVEYDTELRHYAHV
DCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHILLGRQVGVPHIIVFLNKCDMVDDEELLELVEMEVRDLLTQ
YDFPGDNIPIIRGSALKALEGEKIWEDKIIELANSLDKYIPIPVRAVDEPFLLPIEDVFSISGRGTVVTGRIERGILKVG
EEVEIVGIKSTTKTICTGVEMFRKLLDEGRAGENVGILLRGTKREDIERGQVLAKPGTINPHVKFESEVYVLSKEEGGRH
TPFFKGYRPQFYFRTTDVTGSIELPENIEMVMPGDNINMVVTLIHPIAMAEGLRFAIREGGRTVGAGVVTKVIA

Sequences:

>Translated_394_residues
MSKEKFNRSKPHINVGTIGHVDHGKTTLTSAITTVLSKRFGGKACAFEQIDNAPEEKARGITINTSHVEYDTELRHYAHV
DCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHILLGRQVGVPHIIVFLNKCDMVDDEELLELVEMEVRDLLTQ
YDFPGDNIPIIRGSALKALEGEKIWEDKIIELANSLDKYIPIPVRAVDEPFLLPIEDVFSISGRGTVVTGRIERGILKVG
EEVEIVGIKSTTKTICTGVEMFRKLLDEGRAGENVGILLRGTKREDIERGQVLAKPGTINPHVKFESEVYVLSKEEGGRH
TPFFKGYRPQFYFRTTDVTGSIELPENIEMVMPGDNINMVVTLIHPIAMAEGLRFAIREGGRTVGAGVVTKVIA
>Mature_393_residues
SKEKFNRSKPHINVGTIGHVDHGKTTLTSAITTVLSKRFGGKACAFEQIDNAPEEKARGITINTSHVEYDTELRHYAHVD
CPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHILLGRQVGVPHIIVFLNKCDMVDDEELLELVEMEVRDLLTQY
DFPGDNIPIIRGSALKALEGEKIWEDKIIELANSLDKYIPIPVRAVDEPFLLPIEDVFSISGRGTVVTGRIERGILKVGE
EVEIVGIKSTTKTICTGVEMFRKLLDEGRAGENVGILLRGTKREDIERGQVLAKPGTINPHVKFESEVYVLSKEEGGRHT
PFFKGYRPQFYFRTTDVTGSIELPENIEMVMPGDNINMVVTLIHPIAMAEGLRFAIREGGRTVGAGVVTKVIA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=55.4430379746835, Blast_Score=472, Evalue=1e-133,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=29.0178571428571, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI223555963, Length=439, Percent_Identity=28.4738041002278, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI5729864, Length=439, Percent_Identity=28.4738041002278, Blast_Score=151, Evalue=9e-37,
Organism=Homo sapiens, GI4503471, Length=451, Percent_Identity=29.2682926829268, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI54607086, Length=311, Percent_Identity=29.5819935691318, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI194097354, Length=330, Percent_Identity=29.6969696969697, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI194018520, Length=330, Percent_Identity=29.6969696969697, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI194018522, Length=330, Percent_Identity=29.6969696969697, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI46094014, Length=330, Percent_Identity=29.3939393939394, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI4503507, Length=358, Percent_Identity=30.1675977653631, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI94966754, Length=140, Percent_Identity=32.8571428571429, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI82546879, Length=432, Percent_Identity=23.3796296296296, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=86.5482233502538, Blast_Score=679, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=86.5482233502538, Blast_Score=679, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=377, Percent_Identity=28.6472148541114, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789108, Length=337, Percent_Identity=29.673590504451, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI48994988, Length=256, Percent_Identity=32.8125, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=52.3809523809524, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25141371, Length=385, Percent_Identity=44.6753246753247, Blast_Score=338, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI17552884, Length=451, Percent_Identity=29.7117516629712, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17569207, Length=451, Percent_Identity=29.7117516629712, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI115532067, Length=438, Percent_Identity=29.4520547945205, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=435, Percent_Identity=29.4252873563218, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32566303, Length=314, Percent_Identity=30.8917197452229, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=273, Percent_Identity=30.4029304029304, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32566629, Length=355, Percent_Identity=26.4788732394366, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17509919, Length=374, Percent_Identity=29.4117647058824, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=34.2281879194631, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=236, Percent_Identity=27.1186440677966, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=395, Percent_Identity=63.5443037974684, Blast_Score=520, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6325337, Length=440, Percent_Identity=29.5454545454545, Blast_Score=143, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6319594, Length=440, Percent_Identity=29.5454545454545, Blast_Score=143, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6322937, Length=331, Percent_Identity=25.6797583081571, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=292, Percent_Identity=28.7671232876712, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6320377, Length=342, Percent_Identity=28.0701754385965, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=56.4885496183206, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=56.4885496183206, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=50.7772020725389, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI45553807, Length=454, Percent_Identity=28.8546255506608, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI45553816, Length=454, Percent_Identity=28.8546255506608, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24651721, Length=454, Percent_Identity=28.8546255506608, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI17864154, Length=454, Percent_Identity=28.8546255506608, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24652838, Length=455, Percent_Identity=28.7912087912088, Blast_Score=148, Evalue=7e-36,
Organism=Drosophila melanogaster, GI17137572, Length=455, Percent_Identity=28.7912087912088, Blast_Score=148, Evalue=7e-36,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=30.03300330033, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI45550900, Length=348, Percent_Identity=29.0229885057471, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17137380, Length=348, Percent_Identity=29.5977011494253, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24647048, Length=378, Percent_Identity=28.3068783068783, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24647046, Length=378, Percent_Identity=28.3068783068783, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28574573, Length=140, Percent_Identity=34.2857142857143, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28572034, Length=173, Percent_Identity=35.2601156069364, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_BUCCC (Q057A2)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802890.1
- ProteinModelPortal:   Q057A2
- SMR:   Q057A2
- STRING:   Q057A2
- EnsemblBacteria:   EBBUCT00000001336
- GeneID:   4440935
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_343
- eggNOG:   COG0050
- GeneTree:   EBGT00050000007783
- HOGENOM:   HBG307581
- OMA:   RDKPFLM
- ProtClustDB:   PRK00049
- BioCyc:   BAPH372461:BCC_343-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43494; Mature: 43363

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEKFNRSKPHINVGTIGHVDHGKTTLTSAITTVLSKRFGGKACAFEQIDNAPEEKARG
CCCCCCCCCCCEEEECEEECCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHCCCHHHHHCC
ITINTSHVEYDTELRHYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHI
EEEECCCEEECCHHHHEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHE
LLGRQVGVPHIIVFLNKCDMVDDEELLELVEMEVRDLLTQYDFPGDNIPIIRGSALKALE
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHCC
GEKIWEDKIIELANSLDKYIPIPVRAVDEPFLLPIEDVFSISGRGTVVTGRIERGILKVG
CCHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEHHHHHCCCCCCEEEECHHHHHHHHCC
EEVEIVGIKSTTKTICTGVEMFRKLLDEGRAGENVGILLRGTKREDIERGQVLAKPGTIN
CEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHCCCEEEECCCCCC
PHVKFESEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGSIELPENIEMVMPGDNINMV
CCEEECCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCCCEE
VTLIHPIAMAEGLRFAIREGGRTVGAGVVTKVIA
EEEHHHHHHHHHHHHHHHCCCCEECHHHHHHHCC
>Mature Secondary Structure 
SKEKFNRSKPHINVGTIGHVDHGKTTLTSAITTVLSKRFGGKACAFEQIDNAPEEKARG
CCCCCCCCCCEEEECEEECCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHCCCHHHHHCC
ITINTSHVEYDTELRHYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHI
EEEECCCEEECCHHHHEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHE
LLGRQVGVPHIIVFLNKCDMVDDEELLELVEMEVRDLLTQYDFPGDNIPIIRGSALKALE
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHCC
GEKIWEDKIIELANSLDKYIPIPVRAVDEPFLLPIEDVFSISGRGTVVTGRIERGILKVG
CCHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEHHHHHCCCCCCEEEECHHHHHHHHCC
EEVEIVGIKSTTKTICTGVEMFRKLLDEGRAGENVGILLRGTKREDIERGQVLAKPGTIN
CEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHCCCEEEECCCCCC
PHVKFESEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGSIELPENIEMVMPGDNINMV
CCEEECCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCCCEE
VTLIHPIAMAEGLRFAIREGGRTVGAGVVTKVIA
EEEHHHHHHHHHHHHHHHCCCCEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA