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The map label for this gene is trmD [H]

Identifier: 116515180

GI number: 116515180

Start: 282214

End: 282900

Strand: Direct

Name: trmD [H]

Synonym: BCc_248

Alternate gene names: 116515180

Gene position: 282214-282900 (Clockwise)

Preceding gene: 116515179

Following gene: 116515181

Centisome position: 67.78

GC content: 19.94

Gene sequence:

>687_bases
ATGATATATTTTACTGTTATTAGTATTTTTCCAAATTTATTTAAAAATATTTTTAAATATGGAATAATATCCCAAGCAAT
CAAAAAAAAAATAATTCAAATAAATATATTAAATTTAAGAGATTATAGTAATAATAAAAGAAAAAAAATAGATGATAAAG
TATACGGTGGTGGTTCTGGAATGTTAATTATGTTTTTACCTTTATTTTTAGCTGTTAAAACAGCTAAAAAAAAATATGGA
AAAAAAAGTATTGTTATATATTTATCACCACAAGGAAAATTATTAAACCAAAATTATTTAAAAAATTTTATTAAAAATAA
ACATATAATATTTATTTGTGGACGTTATAAAGGTATAGACGAACGTTTTATAAAAACGCAAGTAGATGAAGAATGGTCTA
TTGGAAATTATATTTTAAGTGGGGGAGAAATACCTGCAATAGTATTTATTGATGTAATTACAAGAATGATACCTGGAGTT
CTTAACAATAAAAAATCTTTGATTGAAGAATCATTTTCAAATAATCTTTTAGATTGTCCTAATTATACTAAACCAAAAAA
AATTTTAAATTATTCTGTTCCAAAAGTTTTATTATCAGGAAATCATAAAAAAATAAAAGAATGGAGAATAAAACATGCAT
TAAAAAATACAATTTTAAAAAAACCAAATATTTTAAAAAGAAAATAA

Upstream 100 bases:

>100_bases
TTATACAACCAAATTTCATCAAGAAAATAGATATAAAAAAAAAAATTATAGTTATTGACTTAACTAATTTAAATAACCAA
TTAATTTAAATATACAAAAA

Downstream 100 bases:

>100_bases
TTATATATAATATATAATTAATATTATTTAAAATATCATAAATAAATAAATTTAAACATGAGTCATTACAATGAATAATT
ATATAAGAAATATAGAAAAT

Product: hypothetical protein

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MIYFTVISIFPNLFKNIFKYGIISQAIKKKIIQINILNLRDYSNNKRKKIDDKVYGGGSGMLIMFLPLFLAVKTAKKKYG
KKSIVIYLSPQGKLLNQNYLKNFIKNKHIIFICGRYKGIDERFIKTQVDEEWSIGNYILSGGEIPAIVFIDVITRMIPGV
LNNKKSLIEESFSNNLLDCPNYTKPKKILNYSVPKVLLSGNHKKIKEWRIKHALKNTILKKPNILKRK

Sequences:

>Translated_228_residues
MIYFTVISIFPNLFKNIFKYGIISQAIKKKIIQINILNLRDYSNNKRKKIDDKVYGGGSGMLIMFLPLFLAVKTAKKKYG
KKSIVIYLSPQGKLLNQNYLKNFIKNKHIIFICGRYKGIDERFIKTQVDEEWSIGNYILSGGEIPAIVFIDVITRMIPGV
LNNKKSLIEESFSNNLLDCPNYTKPKKILNYSVPKVLLSGNHKKIKEWRIKHALKNTILKKPNILKRK
>Mature_228_residues
MIYFTVISIFPNLFKNIFKYGIISQAIKKKIIQINILNLRDYSNNKRKKIDDKVYGGGSGMLIMFLPLFLAVKTAKKKYG
KKSIVIYLSPQGKLLNQNYLKNFIKNKHIIFICGRYKGIDERFIKTQVDEEWSIGNYILSGGEIPAIVFIDVITRMIPGV
LNNKKSLIEESFSNNLLDCPNYTKPKKILNYSVPKVLLSGNHKKIKEWRIKHALKNTILKKPNILKRK

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=225, Percent_Identity=46.6666666666667, Blast_Score=246, Evalue=8e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 26382; Mature: 26382

Theoretical pI: Translated: 10.75; Mature: 10.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYFTVISIFPNLFKNIFKYGIISQAIKKKIIQINILNLRDYSNNKRKKIDDKVYGGGSG
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHCCHHHCCCCCC
MLIMFLPLFLAVKTAKKKYGKKSIVIYLSPQGKLLNQNYLKNFIKNKHIIFICGRYKGID
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCCH
ERFIKTQVDEEWSIGNYILSGGEIPAIVFIDVITRMIPGVLNNKKSLIEESFSNNLLDCP
HHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCC
NYTKPKKILNYSVPKVLLSGNHKKIKEWRIKHALKNTILKKPNILKRK
CCCCCHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIYFTVISIFPNLFKNIFKYGIISQAIKKKIIQINILNLRDYSNNKRKKIDDKVYGGGSG
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHCCHHHCCCCCC
MLIMFLPLFLAVKTAKKKYGKKSIVIYLSPQGKLLNQNYLKNFIKNKHIIFICGRYKGID
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCCH
ERFIKTQVDEEWSIGNYILSGGEIPAIVFIDVITRMIPGVLNNKKSLIEESFSNNLLDCP
HHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCC
NYTKPKKILNYSVPKVLLSGNHKKIKEWRIKHALKNTILKKPNILKRK
CCCCCHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]