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The map label for this gene is nusA [H]

Identifier: 116515165

GI number: 116515165

Start: 269027

End: 270514

Strand: Reverse

Name: nusA [H]

Synonym: BCc_232

Alternate gene names: 116515165

Gene position: 270514-269027 (Counterclockwise)

Preceding gene: 116515166

Following gene: 116515164

Centisome position: 64.97

GC content: 24.19

Gene sequence:

>1488_bases
ATGAACAAAGAAATATTATCTGTGGTAGATGCTGTTTCACATGAAAAATCTATTCCAAGAGAAAAGATTTTTCAAGCTTT
AGAAAGTGCTTTAGAAATTGCTACCAAAAAAAAATATAATCAAGATATTAATATTAGAGTATGCATAAATCGTAGTACAG
GTAGTTTTAATACATATAGAAGATGGTTAGTGGTTAATAATGTATTTAATCCAACAAAAGAGATTACATTAGAAGCTGCT
CGATTTGAAAATAAAAAAATTCAATTATGTGATTATATAGAAGATTGCATTGATTCTGTAACTTTTGATCGTATTGCTAC
ACAAATTGCTAAGCAAGTAATAATACAAAAAGTTAGAGAAGCTGAAAAAGAAATAATTCTTAATAAATTCGATAAAAAAA
AAGGTCAAATTATTATAGGTATTGTTAAAAAAATCAGTAGAGATTATATTATTTTAGACGTTGGTAATAATATTGAAGGT
ATTATTATGCGTGAAGATATGTTACCAAGAGAAAATTTTCGAATTAATGATAGAGTTAGAGGTATATTATATAATATTTC
TTATGAAAAACAAGGTGCTCAATTATTTATAAGTAGATCAAAATCTGATATGTTAATTGAATTATTTCGTATTGAAGTAC
CTGAAATTAGAGAAAAATTTATAGAAATTAAAGCAATTGCACGTGATCCTGGTTTACGTTCTAAAATAGCTGTTACCACA
TATGATGAGAGAATTGATCCAGTAGGAGCATGTGTAGGAATGAGGGGTTCTAGAGTTCAAGCTGTTTCTAATGAATTATG
TGGAGAAAGAATTGATATTATTTTATGGGATAATAATCCGAAAAAATTTGTTATTAATTCTATGGCACCCGCTGATGTAT
CTTCTATTTTTTTGGATAATACTAATCATGTAATTAATATAGAAGTTAAATTATGTAATTTAGCACAAGCAATTGGTAGA
AATGGACAAAATGTAAGATTAGCTTCTCAATTAACTGGATGGGAATTAAATATAATTACTTCTAATCCTATTATTAAAAT
AAAAAAATCAAAAAAAAATAATTTTTTTGATATATTAAAAAATAAATTTAATTTTACAGAAAATGATATTTTATTAGTAA
TTCATTCTGGTTTTTCTTCTATTAAATCAATTGCTTATGCTTCTATTAATCAGTTATTATCAATTAAAGGAATTAAAACA
GATGTTGTTTTAAACATGCAGAAAAAAGCTATATTTATTTTAGAAAATGACAAAAAGAAATCTATTAAAATATATAAAAA
AAAATATTTAAATTCTGAATTTGCAAATTTAAAAAATATTAATAAATTTATTATTCAACAGTTAATAGAAAAAAAAATAT
GTACTTTAGAACATCTAGCAGAACAAAGTATTGATGATCTTCATGATATTTCTTTTCTTTTCTTCCGCACAAGCTGGTCA
TTAATTATGGAAGCTCGGAATATTTGTTGGTTTAATGAAAATTCATAA

Upstream 100 bases:

>100_bases
ATAAAAATATTATTTTTTATTTTGATTGAAAAAAATTTTCTTTTTCTTTGAATTCTTTATTTAGTTCAATATATCCATTT
TTAATTTAAGGATAACTGAT

Downstream 100 bases:

>100_bases
AAATAATTAAAATATGAAAATACTTATTAATATAATAAAAGATATATTAATAAGTATTAAATTTTTTTAAAAAAGTTTTG
TAATATTTTTAATATATAAT

Product: NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MNKEILSVVDAVSHEKSIPREKIFQALESALEIATKKKYNQDINIRVCINRSTGSFNTYRRWLVVNNVFNPTKEITLEAA
RFENKKIQLCDYIEDCIDSVTFDRIATQIAKQVIIQKVREAEKEIILNKFDKKKGQIIIGIVKKISRDYIILDVGNNIEG
IIMREDMLPRENFRINDRVRGILYNISYEKQGAQLFISRSKSDMLIELFRIEVPEIREKFIEIKAIARDPGLRSKIAVTT
YDERIDPVGACVGMRGSRVQAVSNELCGERIDIILWDNNPKKFVINSMAPADVSSIFLDNTNHVINIEVKLCNLAQAIGR
NGQNVRLASQLTGWELNIITSNPIIKIKKSKKNNFFDILKNKFNFTENDILLVIHSGFSSIKSIAYASINQLLSIKGIKT
DVVLNMQKKAIFILENDKKKSIKIYKKKYLNSEFANLKNINKFIIQQLIEKKICTLEHLAEQSIDDLHDISFLFFRTSWS
LIMEARNICWFNENS

Sequences:

>Translated_495_residues
MNKEILSVVDAVSHEKSIPREKIFQALESALEIATKKKYNQDINIRVCINRSTGSFNTYRRWLVVNNVFNPTKEITLEAA
RFENKKIQLCDYIEDCIDSVTFDRIATQIAKQVIIQKVREAEKEIILNKFDKKKGQIIIGIVKKISRDYIILDVGNNIEG
IIMREDMLPRENFRINDRVRGILYNISYEKQGAQLFISRSKSDMLIELFRIEVPEIREKFIEIKAIARDPGLRSKIAVTT
YDERIDPVGACVGMRGSRVQAVSNELCGERIDIILWDNNPKKFVINSMAPADVSSIFLDNTNHVINIEVKLCNLAQAIGR
NGQNVRLASQLTGWELNIITSNPIIKIKKSKKNNFFDILKNKFNFTENDILLVIHSGFSSIKSIAYASINQLLSIKGIKT
DVVLNMQKKAIFILENDKKKSIKIYKKKYLNSEFANLKNINKFIIQQLIEKKICTLEHLAEQSIDDLHDISFLFFRTSWS
LIMEARNICWFNENS
>Mature_495_residues
MNKEILSVVDAVSHEKSIPREKIFQALESALEIATKKKYNQDINIRVCINRSTGSFNTYRRWLVVNNVFNPTKEITLEAA
RFENKKIQLCDYIEDCIDSVTFDRIATQIAKQVIIQKVREAEKEIILNKFDKKKGQIIIGIVKKISRDYIILDVGNNIEG
IIMREDMLPRENFRINDRVRGILYNISYEKQGAQLFISRSKSDMLIELFRIEVPEIREKFIEIKAIARDPGLRSKIAVTT
YDERIDPVGACVGMRGSRVQAVSNELCGERIDIILWDNNPKKFVINSMAPADVSSIFLDNTNHVINIEVKLCNLAQAIGR
NGQNVRLASQLTGWELNIITSNPIIKIKKSKKNNFFDILKNKFNFTENDILLVIHSGFSSIKSIAYASINQLLSIKGIKT
DVVLNMQKKAIFILENDKKKSIKIYKKKYLNSEFANLKNINKFIIQQLIEKKICTLEHLAEQSIDDLHDISFLFFRTSWS
LIMEARNICWFNENS

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=495, Percent_Identity=55.959595959596, Blast_Score=588, Evalue=1e-169,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 57047; Mature: 57047

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEILSVVDAVSHEKSIPREKIFQALESALEIATKKKYNQDINIRVCINRSTGSFNTYR
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHE
RWLVVNNVFNPTKEITLEAARFENKKIQLCDYIEDCIDSVTFDRIATQIAKQVIIQKVRE
EEEEEECCCCCCHHHEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEKEIILNKFDKKKGQIIIGIVKKISRDYIILDVGNNIEGIIMREDMLPRENFRINDRVR
HHHHHHHHHCCCCCCCEEEEEHHHHCCCEEEEECCCCCCEEEEECCCCCCCCCEECCCEE
GILYNISYEKQGAQLFISRSKSDMLIELFRIEVPEIREKFIEIKAIARDPGLRSKIAVTT
EEEEEEEECCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHEECCCCCCCEEEEEE
YDERIDPVGACVGMRGSRVQAVSNELCGERIDIILWDNNPKKFVINSMAPADVSSIFLDN
CHHCCCCHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCCCHHEEECC
TNHVINIEVKLCNLAQAIGRNGQNVRLASQLTGWELNIITSNPIIKIKKSKKNNFFDILK
CCEEEEEEEEEEHHHHHHCCCCCCEEEEEECCCEEEEEEECCCEEEEEECCCCCHHHHHH
NKFNFTENDILLVIHSGFSSIKSIAYASINQLLSIKGIKTDVVLNMQKKAIFILENDKKK
HHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCC
SIKIYKKKYLNSEFANLKNINKFIIQQLIEKKICTLEHLAEQSIDDLHDISFLFFRTSWS
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHH
LIMEARNICWFNENS
HHHHHHHEEEECCCC
>Mature Secondary Structure
MNKEILSVVDAVSHEKSIPREKIFQALESALEIATKKKYNQDINIRVCINRSTGSFNTYR
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHE
RWLVVNNVFNPTKEITLEAARFENKKIQLCDYIEDCIDSVTFDRIATQIAKQVIIQKVRE
EEEEEECCCCCCHHHEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEKEIILNKFDKKKGQIIIGIVKKISRDYIILDVGNNIEGIIMREDMLPRENFRINDRVR
HHHHHHHHHCCCCCCCEEEEEHHHHCCCEEEEECCCCCCEEEEECCCCCCCCCEECCCEE
GILYNISYEKQGAQLFISRSKSDMLIELFRIEVPEIREKFIEIKAIARDPGLRSKIAVTT
EEEEEEEECCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHEECCCCCCCEEEEEE
YDERIDPVGACVGMRGSRVQAVSNELCGERIDIILWDNNPKKFVINSMAPADVSSIFLDN
CHHCCCCHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCCCHHEEECC
TNHVINIEVKLCNLAQAIGRNGQNVRLASQLTGWELNIITSNPIIKIKKSKKNNFFDILK
CCEEEEEEEEEEHHHHHHCCCCCCEEEEEECCCEEEEEEECCCEEEEEECCCCCHHHHHH
NKFNFTENDILLVIHSGFSSIKSIAYASINQLLSIKGIKTDVVLNMQKKAIFILENDKKK
HHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCC
SIKIYKKKYLNSEFANLKNINKFIIQQLIEKKICTLEHLAEQSIDDLHDISFLFFRTSWS
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHH
LIMEARNICWFNENS
HHHHHHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]