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The map label for this gene is argF [C]

Identifier: 116515157

GI number: 116515157

Start: 259090

End: 260091

Strand: Direct

Name: argF [C]

Synonym: BCc_224

Alternate gene names: 116515157

Gene position: 259090-260091 (Clockwise)

Preceding gene: 116515153

Following gene: 116515158

Centisome position: 62.22

GC content: 21.16

Gene sequence:

>1002_bases
ATGAATACAATTTATAAAAAAAGTTTTTTAAAATTATCAGATCTTAATTACCAAGAAATTATTTATTTAATTAAATTATC
AAAATTTTTTAAAAAAAAAAAAAAAGATAAAAAGGAAAAAAAATATTTAAAAGGAAAAAATATAGTATTAATTTTTGAAA
AACAATCTACTAGAACTAGATGTGCATTTGAAGTAGCTGCTTACGATCAAGGAGCTAATACAACTTATTTAAGTCCTACA
GATACACATATGGGATATAAAGAATCAATTCATGATACTGCTAAAGTATTAGGAAAAATGTATGATGGAATACAATATCG
AGGTTATCATGATAATATTCTTAAAAAAATACAAAAATATTCAAAAATACCTGTTTGGAATGGATTAACTAATACATTTC
ATCCTACTCAAATTTTAGCAGATCTATTAACTATGACTGAAACATTACCAAAAAAATCAATTCAAGAAATTACATGTGCA
TATATAGGTGATGCTAAAAATAATATCGCAAATACATTATTAGAAGCTGCTAATATAACTGGATTAAAATTAAATATAAT
CTCTCCAGAAAACTATTGGCCTAAAAAAAATATTAGAGATATAAAAAATAAAAAAAATATACTTTATACTAAAAATATAG
AAAAAGGCGTATATAAAGTAGATTTTATTTATACTGATGTATGGGTGTCTATGGGAGATAAATCAATGAAATGGAAAAAA
AAAATAAAAGAATTATATGATTATCAAGTTAACCAAAAAATGTTGAATATGACAAAAAATTCAAATGTAAAAATACTTCA
TTGTCTTCCTGCTTTACATGATAAAGAATCTACATTAGGATTAAAAATACATAAAAAATATAATTTAAAAAATGGATTAG
AAATTTCTAATAATGTTTTTTTTTCTAAAAAAAATCTTTGTTTTGAACAATCAGAAAATAGATTACATACAATTAAAGCC
TTATTAGTTTCATGTTTGCATAAAAAAAAAATTTTAATATAA

Upstream 100 bases:

>100_bases
ATTTATTTATAAAATAATTATTTTTTAAATTTTATAAAATTCTTTAATATTTAAATTATTAATAAATTTTTAATAATATA
TTTATATATGAGATATATAC

Downstream 100 bases:

>100_bases
AATATTCATTTATATATTTGGATATAATATAATAATATAAAAGAGAAACTTATGTTTAAAAAAAATATAGTATTTGGTCC
TTATTCACCTTGTATAAAAA

Product: hypothetical protein

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MNTIYKKSFLKLSDLNYQEIIYLIKLSKFFKKKKKDKKEKKYLKGKNIVLIFEKQSTRTRCAFEVAAYDQGANTTYLSPT
DTHMGYKESIHDTAKVLGKMYDGIQYRGYHDNILKKIQKYSKIPVWNGLTNTFHPTQILADLLTMTETLPKKSIQEITCA
YIGDAKNNIANTLLEAANITGLKLNIISPENYWPKKNIRDIKNKKNILYTKNIEKGVYKVDFIYTDVWVSMGDKSMKWKK
KIKELYDYQVNQKMLNMTKNSNVKILHCLPALHDKESTLGLKIHKKYNLKNGLEISNNVFFSKKNLCFEQSENRLHTIKA
LLVSCLHKKKILI

Sequences:

>Translated_333_residues
MNTIYKKSFLKLSDLNYQEIIYLIKLSKFFKKKKKDKKEKKYLKGKNIVLIFEKQSTRTRCAFEVAAYDQGANTTYLSPT
DTHMGYKESIHDTAKVLGKMYDGIQYRGYHDNILKKIQKYSKIPVWNGLTNTFHPTQILADLLTMTETLPKKSIQEITCA
YIGDAKNNIANTLLEAANITGLKLNIISPENYWPKKNIRDIKNKKNILYTKNIEKGVYKVDFIYTDVWVSMGDKSMKWKK
KIKELYDYQVNQKMLNMTKNSNVKILHCLPALHDKESTLGLKIHKKYNLKNGLEISNNVFFSKKNLCFEQSENRLHTIKA
LLVSCLHKKKILI
>Mature_333_residues
MNTIYKKSFLKLSDLNYQEIIYLIKLSKFFKKKKKDKKEKKYLKGKNIVLIFEKQSTRTRCAFEVAAYDQGANTTYLSPT
DTHMGYKESIHDTAKVLGKMYDGIQYRGYHDNILKKIQKYSKIPVWNGLTNTFHPTQILADLLTMTETLPKKSIQEITCA
YIGDAKNNIANTLLEAANITGLKLNIISPENYWPKKNIRDIKNKKNILYTKNIEKGVYKVDFIYTDVWVSMGDKSMKWKK
KIKELYDYQVNQKMLNMTKNSNVKILHCLPALHDKESTLGLKIHKKYNLKNGLEISNNVFFSKKNLCFEQSENRLHTIKA
LLVSCLHKKKILI

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=327, Percent_Identity=33.0275229357798, Blast_Score=174, Evalue=9e-44,
Organism=Escherichia coli, GI1790703, Length=334, Percent_Identity=53.2934131736527, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1786469, Length=332, Percent_Identity=50.9036144578313, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI2367364, Length=340, Percent_Identity=26.4705882352941, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI193204318, Length=261, Percent_Identity=26.0536398467433, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322373, Length=332, Percent_Identity=30.7228915662651, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6322331, Length=238, Percent_Identity=26.4705882352941, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 38827; Mature: 38827

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTIYKKSFLKLSDLNYQEIIYLIKLSKFFKKKKKDKKEKKYLKGKNIVLIFEKQSTRTR
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
CAFEVAAYDQGANTTYLSPTDTHMGYKESIHDTAKVLGKMYDGIQYRGYHDNILKKIQKY
EEEEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHH
SKIPVWNGLTNTFHPTQILADLLTMTETLPKKSIQEITCAYIGDAKNNIANTLLEAANIT
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
GLKLNIISPENYWPKKNIRDIKNKKNILYTKNIEKGVYKVDFIYTDVWVSMGDKSMKWKK
EEEEEEECCCCCCCCCCHHHHHCCCCEEEECCCCCCCEEEEEEEEEHHHHCCCCCHHHHH
KIKELYDYQVNQKMLNMTKNSNVKILHCLPALHDKESTLGLKIHKKYNLKNGLEISNNVF
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCCCEECCCEE
FSKKNLCFEQSENRLHTIKALLVSCLHKKKILI
EECCCCCHHHCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNTIYKKSFLKLSDLNYQEIIYLIKLSKFFKKKKKDKKEKKYLKGKNIVLIFEKQSTRTR
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
CAFEVAAYDQGANTTYLSPTDTHMGYKESIHDTAKVLGKMYDGIQYRGYHDNILKKIQKY
EEEEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHH
SKIPVWNGLTNTFHPTQILADLLTMTETLPKKSIQEITCAYIGDAKNNIANTLLEAANIT
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
GLKLNIISPENYWPKKNIRDIKNKKNILYTKNIEKGVYKVDFIYTDVWVSMGDKSMKWKK
EEEEEEECCCCCCCCCCHHHHHCCCCEEEECCCCCCCEEEEEEEEEHHHHCCCCCHHHHH
KIKELYDYQVNQKMLNMTKNSNVKILHCLPALHDKESTLGLKIHKKYNLKNGLEISNNVF
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCCCEECCCEE
FSKKNLCFEQSENRLHTIKALLVSCLHKKKILI
EECCCCCHHHCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12886019 [H]