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The map label for this gene is holB [H]

Identifier: 116515152

GI number: 116515152

Start: 250965

End: 251942

Strand: Direct

Name: holB [H]

Synonym: BCc_219

Alternate gene names: 116515152

Gene position: 250965-251942 (Clockwise)

Preceding gene: 116515151

Following gene: 116515153

Centisome position: 60.27

GC content: 16.26

Gene sequence:

>978_bases
ATGAACCTCTATCCTTGGTTAGTTAACTACTATAAAAATATAATTAAAAAATATCACATTAGTAAACTTCATCCAATTAT
TTTAATAAATTCACCGATAGGAACCGGAATATCAAATTTGATTTTAAACATTTGTCAATGGATATTATGTCTTTATAAAA
ACAAAGAAAAATATTGTAATAATTGCTCATCTTGTTTATTAATAAAAAATAATGTACATCCAGATTTTTATTTTATTAAA
AATAGTTTTAGAAAAAAATATATTAAAATTAATAAAATTAGAAATATAATTAAATGTATATATCAAATGTCTCAACAAGG
GAAGGAAAAGATTATTTATTTCCAAAATATTAATTATCTTACTCCTGAAGCAAGTAGTTCTCTATTAAAAACATTAGAAG
AACCTCCAAAAAATACTATATTTTTTTTACGTACTACAAATATCTCAAAAATTGATCATACGATTAAAAGTAGATCTATA
ATCTATTATATTAATACTTTAGATGAAAATAAAAATTTATTTTGGTTAAAAAAAAAAAATAAAAATTATAAAAAAAAAAA
AATATTAACAGCATTAAGAATAAATAATAATATACCATTAATTACACATAAATTTTTAAATACATATTTATTAAATGAAA
GAAAAAATTTTATGTTAGAAATATATAATAATACAATTAATAAAAGTAACGTTAATTTAATACAATTAATTAATACATAT
CACGAAGATAAAATAGCTAACTGGATTATTTGTTTAATATTAGATGTTATAAAATACAAAACATATAATTCTAAAAAAAT
AATAAATTTAGATCAAATTAAGTTAATTAAAAAAATTGCGAAAATTAATAATATTAAAAAATTAAATAAACATATAAAAT
CTTGGATTATATATAAAAGAATATTATTTTATTCAGATTATATTGATAAAAAATTAATTTTTACAGAACAAATTTTATTA
TTTTCAACTCTTTTATAA

Upstream 100 bases:

>100_bases
TTAACATTAAATTATTTGATATTGAAGCGGAAAAAATAAAAAAAATTAAAGATATAATTCCGTATATAAAAAAAAAAAAA
CTAAAAGAATAAATATTCTT

Downstream 100 bases:

>100_bases
AAAGAAATTATATATTTAATATCTTTTAACAAGGAAATAAAAATGTTTTTAATTGATACACATTGTCATATTCATAATAT
ATGTCAATTAGATAAAAAAA

Product: DNA polymerase III, d' subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MNLYPWLVNYYKNIIKKYHISKLHPIILINSPIGTGISNLILNICQWILCLYKNKEKYCNNCSSCLLIKNNVHPDFYFIK
NSFRKKYIKINKIRNIIKCIYQMSQQGKEKIIYFQNINYLTPEASSSLLKTLEEPPKNTIFFLRTTNISKIDHTIKSRSI
IYYINTLDENKNLFWLKKKNKNYKKKKILTALRINNNIPLITHKFLNTYLLNERKNFMLEIYNNTINKSNVNLIQLINTY
HEDKIANWIICLILDVIKYKTYNSKKIINLDQIKLIKKIAKINNIKKLNKHIKSWIIYKRILFYSDYIDKKLIFTEQILL
FSTLL

Sequences:

>Translated_325_residues
MNLYPWLVNYYKNIIKKYHISKLHPIILINSPIGTGISNLILNICQWILCLYKNKEKYCNNCSSCLLIKNNVHPDFYFIK
NSFRKKYIKINKIRNIIKCIYQMSQQGKEKIIYFQNINYLTPEASSSLLKTLEEPPKNTIFFLRTTNISKIDHTIKSRSI
IYYINTLDENKNLFWLKKKNKNYKKKKILTALRINNNIPLITHKFLNTYLLNERKNFMLEIYNNTINKSNVNLIQLINTY
HEDKIANWIICLILDVIKYKTYNSKKIINLDQIKLIKKIAKINNIKKLNKHIKSWIIYKRILFYSDYIDKKLIFTEQILL
FSTLL
>Mature_325_residues
MNLYPWLVNYYKNIIKKYHISKLHPIILINSPIGTGISNLILNICQWILCLYKNKEKYCNNCSSCLLIKNNVHPDFYFIK
NSFRKKYIKINKIRNIIKCIYQMSQQGKEKIIYFQNINYLTPEASSSLLKTLEEPPKNTIFFLRTTNISKIDHTIKSRSI
IYYINTLDENKNLFWLKKKNKNYKKKKILTALRINNNIPLITHKFLNTYLLNERKNFMLEIYNNTINKSNVNLIQLINTY
HEDKIANWIICLILDVIKYKTYNSKKIINLDQIKLIKKIAKINNIKKLNKHIKSWIIYKRILFYSDYIDKKLIFTEQILL
FSTLL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=321, Percent_Identity=23.98753894081, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1786676, Length=173, Percent_Identity=22.5433526011561, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 38993; Mature: 38993

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLYPWLVNYYKNIIKKYHISKLHPIILINSPIGTGISNLILNICQWILCLYKNKEKYCN
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHC
NCSSCLLIKNNVHPDFYFIKNSFRKKYIKINKIRNIIKCIYQMSQQGKEKIIYFQNINYL
CCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEE
TPEASSSLLKTLEEPPKNTIFFLRTTNISKIDHTIKSRSIIYYINTLDENKNLFWLKKKN
CCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCEEEEEEEEECCCCCEEEEECCC
KNYKKKKILTALRINNNIPLITHKFLNTYLLNERKNFMLEIYNNTINKSNVNLIQLINTY
CCCHHHHHHHHEEECCCCCEEHHHHHHHHHHCCCHHHEEEEECCCCCCCCCHHHHHHHHH
HEDKIANWIICLILDVIKYKTYNSKKIINLDQIKLIKKIAKINNIKKLNKHIKSWIIYKR
HHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILFYSDYIDKKLIFTEQILLFSTLL
HHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLYPWLVNYYKNIIKKYHISKLHPIILINSPIGTGISNLILNICQWILCLYKNKEKYCN
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHC
NCSSCLLIKNNVHPDFYFIKNSFRKKYIKINKIRNIIKCIYQMSQQGKEKIIYFQNINYL
CCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEE
TPEASSSLLKTLEEPPKNTIFFLRTTNISKIDHTIKSRSIIYYINTLDENKNLFWLKKKN
CCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCEEEEEEEEECCCCCEEEEECCC
KNYKKKKILTALRINNNIPLITHKFLNTYLLNERKNFMLEIYNNTINKSNVNLIQLINTY
CCCHHHHHHHHEEECCCCCEEHHHHHHHHHHCCCHHHEEEEECCCCCCCCCHHHHHHHHH
HEDKIANWIICLILDVIKYKTYNSKKIINLDQIKLIKKIAKINNIKKLNKHIKSWIIYKR
HHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILFYSDYIDKKLIFTEQILLFSTLL
HHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430; 10491164 [H]