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The map label for this gene is pfkA

Identifier: 116515123

GI number: 116515123

Start: 220001

End: 220963

Strand: Direct

Name: pfkA

Synonym: BCc_187

Alternate gene names: 116515123

Gene position: 220001-220963 (Clockwise)

Preceding gene: 116515122

Following gene: 116515130

Centisome position: 52.84

GC content: 24.61

Gene sequence:

>963_bases
ATGATTAAAAAAATAGGAATTTTAACTAGTGGTGGTGATTCTCCAGGAATGAATGCTATTATTCGATCTGTTGTATGTAC
TGCTTTAAATCATAATATAGAAGTTAAAGGTATATATAACGGATTTTTAGGATTATATAATAATGATATGAAATTATTAA
AATATAGTCATGTTGCTAATTTATTAAATCAAGGTGGAACAATTTTAGGATCTTCTCGATTTTTAGAATTTAAATTAGAA
AAAACAAGAAAAATAGCTATAAAAAACATTTATAAAAGAAAAATTGATTGTTTAATCATTATTGGAGGAGATGGATCTTA
TAAAGCAGCAAATTTTTTAACAAATATGGGTGTTCCATGTATTGGTATTCCCGGAACTATAGATAATGATGTTTCTGGAA
CTGATTATACTGTTGGATATTTTACTGCTTTAGAAACAGTAGTAGATGCTATTGATAAATTAAGAGATACTTCTGCATCA
CATCATAGAATTTCAATAATAGAAATTATGGGTCGTTATTGTGGAGATTTAACATTATCAGCTGCTATTGCCGGTGGTTG
TGAATTTATAGTTATTCCAGAAATAATTTATCAAAAAGAATTATTATTACTAGAAATACAAAAAAGTATTAAAAAAGGTA
ATAAACATTCTATTGTAGCTATTACTGAACATATATGTGATGTAAATAAATTAGCAAAATATATTCAAAAAAAAACTTTT
AAAGAAACAAGAGCAACCATATTAGGTCATATTCAAAGAGGAGGGAAACCTGTTGCATATGATAGAATTTTAGCATCTCG
TATGGGTATTTATGCAATTCAATTACTTCTAAAAGGATATAAAGGACAATGTATAGGAGTAATTAATAATAAAATAGTTC
ATCATAATATCAATTATGCTTTAAAAAATATGAAAAAAATATTTAAAAAAGATTTATTAAATTCTATAAAAAAAATATAC
TAA

Upstream 100 bases:

>100_bases
TATATAAATTTATTAGTTTATATATTTTTAAAAATTGAAAATATCAAAAATTTTAATAATTTAATAAATGTAAATAAAAT
TAGAGTGTTTAAACTATTAC

Downstream 100 bases:

>100_bases
ATTATTTTTTTAAAAAAAATATAAGTGCCGGAATACCGGCGCTTAAATATAATTAAATTATTTGTAATAAATTAAATAAA
AATTTTTAAATATATTTAAT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MIKKIGILTSGGDSPGMNAIIRSVVCTALNHNIEVKGIYNGFLGLYNNDMKLLKYSHVANLLNQGGTILGSSRFLEFKLE
KTRKIAIKNIYKRKIDCLIIIGGDGSYKAANFLTNMGVPCIGIPGTIDNDVSGTDYTVGYFTALETVVDAIDKLRDTSAS
HHRISIIEIMGRYCGDLTLSAAIAGGCEFIVIPEIIYQKELLLLEIQKSIKKGNKHSIVAITEHICDVNKLAKYIQKKTF
KETRATILGHIQRGGKPVAYDRILASRMGIYAIQLLLKGYKGQCIGVINNKIVHHNINYALKNMKKIFKKDLLNSIKKIY

Sequences:

>Translated_320_residues
MIKKIGILTSGGDSPGMNAIIRSVVCTALNHNIEVKGIYNGFLGLYNNDMKLLKYSHVANLLNQGGTILGSSRFLEFKLE
KTRKIAIKNIYKRKIDCLIIIGGDGSYKAANFLTNMGVPCIGIPGTIDNDVSGTDYTVGYFTALETVVDAIDKLRDTSAS
HHRISIIEIMGRYCGDLTLSAAIAGGCEFIVIPEIIYQKELLLLEIQKSIKKGNKHSIVAITEHICDVNKLAKYIQKKTF
KETRATILGHIQRGGKPVAYDRILASRMGIYAIQLLLKGYKGQCIGVINNKIVHHNINYALKNMKKIFKKDLLNSIKKIY
>Mature_320_residues
MIKKIGILTSGGDSPGMNAIIRSVVCTALNHNIEVKGIYNGFLGLYNNDMKLLKYSHVANLLNQGGTILGSSRFLEFKLE
KTRKIAIKNIYKRKIDCLIIIGGDGSYKAANFLTNMGVPCIGIPGTIDNDVSGTDYTVGYFTALETVVDAIDKLRDTSAS
HHRISIIEIMGRYCGDLTLSAAIAGGCEFIVIPEIIYQKELLLLEIQKSIKKGNKHSIVAITEHICDVNKLAKYIQKKTF
KETRATILGHIQRGGKPVAYDRILASRMGIYAIQLLLKGYKGQCIGVINNKIVHHNINYALKNMKKIFKKDLLNSIKKIY

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI11321601, Length=313, Percent_Identity=34.8242811501597, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI48762920, Length=312, Percent_Identity=35.5769230769231, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI266453768, Length=312, Percent_Identity=33.974358974359, Blast_Score=181, Evalue=5e-46,
Organism=Homo sapiens, GI266453748, Length=312, Percent_Identity=33.974358974359, Blast_Score=181, Evalue=5e-46,
Organism=Homo sapiens, GI4505749, Length=312, Percent_Identity=33.974358974359, Blast_Score=181, Evalue=5e-46,
Organism=Homo sapiens, GI266453619, Length=312, Percent_Identity=33.974358974359, Blast_Score=181, Evalue=7e-46,
Organism=Escherichia coli, GI1790350, Length=320, Percent_Identity=64.0625, Blast_Score=447, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17558788, Length=308, Percent_Identity=36.6883116883117, Blast_Score=185, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI25147584, Length=311, Percent_Identity=34.7266881028939, Blast_Score=179, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI72003601, Length=326, Percent_Identity=30.6748466257669, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321679, Length=344, Percent_Identity=34.3023255813954, Blast_Score=198, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6323861, Length=346, Percent_Identity=32.0809248554913, Blast_Score=169, Evalue=6e-43,
Organism=Drosophila melanogaster, GI28573326, Length=314, Percent_Identity=34.7133757961783, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24652337, Length=314, Percent_Identity=34.0764331210191, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI17647809, Length=314, Percent_Identity=34.0764331210191, Blast_Score=180, Evalue=1e-45,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_BUCCC (Q057P0)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802752.1
- ProteinModelPortal:   Q057P0
- SMR:   Q057P0
- STRING:   Q057P0
- EnsemblBacteria:   EBBUCT00000001551
- GeneID:   4440798
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_187
- eggNOG:   COG0205
- GeneTree:   EBGT00050000007904
- HOGENOM:   HBG405044
- OMA:   RCEEFKT
- BioCyc:   BAPH372461:BCC_187-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 35450; Mature: 35450

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 128-128 BINDING 163-163 BINDING 244-244 BINDING 250-250 BINDING 253-253

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKIGILTSGGDSPGMNAIIRSVVCTALNHNIEVKGIYNGFLGLYNNDMKLLKYSHVAN
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCHHHHCCCCHHHHHHHHHHH
LLNQGGTILGSSRFLEFKLEKTRKIAIKNIYKRKIDCLIIIGGDGSYKAANFLTNMGVPC
HHHCCCCEEECCEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCE
IGIPGTIDNDVSGTDYTVGYFTALETVVDAIDKLRDTSASHHRISIIEIMGRYCGDLTLS
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHH
AAIAGGCEFIVIPEIIYQKELLLLEIQKSIKKGNKHSIVAITEHICDVNKLAKYIQKKTF
HHHHCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCHHHHHHHHHHHHH
KETRATILGHIQRGGKPVAYDRILASRMGIYAIQLLLKGYKGQCIGVINNKIVHHNINYA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEECHHHH
LKNMKKIFKKDLLNSIKKIY
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKKIGILTSGGDSPGMNAIIRSVVCTALNHNIEVKGIYNGFLGLYNNDMKLLKYSHVAN
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCHHHHCCCCHHHHHHHHHHH
LLNQGGTILGSSRFLEFKLEKTRKIAIKNIYKRKIDCLIIIGGDGSYKAANFLTNMGVPC
HHHCCCCEEECCEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCE
IGIPGTIDNDVSGTDYTVGYFTALETVVDAIDKLRDTSASHHRISIIEIMGRYCGDLTLS
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHH
AAIAGGCEFIVIPEIIYQKELLLLEIQKSIKKGNKHSIVAITEHICDVNKLAKYIQKKTF
HHHHCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCHHHHHHHHHHHHH
KETRATILGHIQRGGKPVAYDRILASRMGIYAIQLLLKGYKGQCIGVINNKIVHHNINYA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEECHHHH
LKNMKKIFKKDLLNSIKKIY
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA