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The map label for this gene is rnt

Identifier: 116515062

GI number: 116515062

Start: 138471

End: 139136

Strand: Reverse

Name: rnt

Synonym: BCc_122

Alternate gene names: 116515062

Gene position: 139136-138471 (Counterclockwise)

Preceding gene: 116515065

Following gene: 116515060

Centisome position: 33.42

GC content: 27.63

Gene sequence:

>666_bases
ATGTTTATATATAAAGATATAAATTATGCAATAAATAATAGATTTAGAGGTTTTTATCCAGTTGTTATAGATATAGAAAG
CGCAGGATTTCAAGCAGATACAGATGCACTTTTAGAAATTGCAATTGTAACTTTAAAAATGAATAAATCAGGTTGGCTTA
AAATAGATGATAGATTACATTTTCATATTATACCATTTAAAGGATCTATTATAAAAGCTGAATCTGTTGCTTTTAATAAA
ATTGATCCATTTAATCCTCTTAGAGGAGCTGTAAGTGAAAAAAATGCATTAAAAAATATTTTTAAATTGGTTAGAAAAAA
AATTAATATAAATAAATGTAGAAAAGGAATTTTAGTAGCTCATAATGCAAATTTTGATCATAATTTTTTAATGGCTGCAT
CAAAAAGAGTTGGTAATCTTAACAATCCATTTCATCCATTTACTACATTTGATACTGCAGCACTTAGCGGTTTAATTTTT
GGGCAAACAGTTTTAGCAAAAGCATGCAAACTTGCTGGAATTCCATTTGATACAAATCAAGCTCATTCAGCTTTATATGA
TACTATACAAACAGCATATCTTTTTTGTGAATTAGTAAATCGTTGGAAAAGATTAGGAGGATGGCCGCCAAATTCATCTC
ACCGTAAAAAAACCGATGAGATATAA

Upstream 100 bases:

>100_bases
AATTGATTGAGTTATTTAAATAAGATGTTTATAATTTCAAAGTATTAAAATATCATAAAATATACTTCATACTATATTAT
ATACCGAAATTGAGATTCAT

Downstream 100 bases:

>100_bases
AATATTTTTTTTTAAAATTAATAATTATAATTATTTTTAAAAATATTATTTTTTTTTAATTAAATCAAGCAATTCTTTAT
TGTAAAACTTTTCTAATATA

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MFIYKDINYAINNRFRGFYPVVIDIESAGFQADTDALLEIAIVTLKMNKSGWLKIDDRLHFHIIPFKGSIIKAESVAFNK
IDPFNPLRGAVSEKNALKNIFKLVRKKININKCRKGILVAHNANFDHNFLMAASKRVGNLNNPFHPFTTFDTAALSGLIF
GQTVLAKACKLAGIPFDTNQAHSALYDTIQTAYLFCELVNRWKRLGGWPPNSSHRKKTDEI

Sequences:

>Translated_221_residues
MFIYKDINYAINNRFRGFYPVVIDIESAGFQADTDALLEIAIVTLKMNKSGWLKIDDRLHFHIIPFKGSIIKAESVAFNK
IDPFNPLRGAVSEKNALKNIFKLVRKKININKCRKGILVAHNANFDHNFLMAASKRVGNLNNPFHPFTTFDTAALSGLIF
GQTVLAKACKLAGIPFDTNQAHSALYDTIQTAYLFCELVNRWKRLGGWPPNSSHRKKTDEI
>Mature_221_residues
MFIYKDINYAINNRFRGFYPVVIDIESAGFQADTDALLEIAIVTLKMNKSGWLKIDDRLHFHIIPFKGSIIKAESVAFNK
IDPFNPLRGAVSEKNALKNIFKLVRKKININKCRKGILVAHNANFDHNFLMAASKRVGNLNNPFHPFTTFDTAALSGLIF
GQTVLAKACKLAGIPFDTNQAHSALYDTIQTAYLFCELVNRWKRLGGWPPNSSHRKKTDEI

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain

Homologues:

Organism=Escherichia coli, GI1787941, Length=203, Percent_Identity=64.5320197044335, Blast_Score=286, Evalue=5e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNT_BUCCC (Q057V1)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802691.1
- ProteinModelPortal:   Q057V1
- SMR:   Q057V1
- STRING:   Q057V1
- EnsemblBacteria:   EBBUCT00000001481
- GeneID:   4440832
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_122
- eggNOG:   COG0847
- GeneTree:   EBGT00050000008097
- HOGENOM:   HBG289562
- OMA:   YDAEKTA
- ProtClustDB:   PRK05168
- BioCyc:   BAPH372461:BCC_122-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00157
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337
- SMART:   SM00479
- TIGRFAMs:   TIGR01298

Pfam domain/function: PF00929 Exonuc_X-T; SSF53098 RNaseH_fold

EC number: 3.1.13.-

Molecular weight: Translated: 24970; Mature: 24970

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIYKDINYAINNRFRGFYPVVIDIESAGFQADTDALLEIAIVTLKMNKSGWLKIDDRLH
CEEEECCCHHHCCCCCCEEEEEEEEECCCCCCCHHHHEEEEEEEEEECCCCCEEECCEEE
FHIIPFKGSIIKAESVAFNKIDPFNPLRGAVSEKNALKNIFKLVRKKININKCRKGILVA
EEEEECCCCEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEE
HNANFDHNFLMAASKRVGNLNNPFHPFTTFDTAALSGLIFGQTVLAKACKLAGIPFDTNQ
ECCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
AHSALYDTIQTAYLFCELVNRWKRLGGWPPNSSHRKKTDEI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFIYKDINYAINNRFRGFYPVVIDIESAGFQADTDALLEIAIVTLKMNKSGWLKIDDRLH
CEEEECCCHHHCCCCCCEEEEEEEEECCCCCCCHHHHEEEEEEEEEECCCCCEEECCEEE
FHIIPFKGSIIKAESVAFNKIDPFNPLRGAVSEKNALKNIFKLVRKKININKCRKGILVA
EEEEECCCCEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEE
HNANFDHNFLMAASKRVGNLNNPFHPFTTFDTAALSGLIFGQTVLAKACKLAGIPFDTNQ
ECCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
AHSALYDTIQTAYLFCELVNRWKRLGGWPPNSSHRKKTDEI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA