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The map label for this gene is nuoI

Identifier: 116515045

GI number: 116515045

Start: 119859

End: 120404

Strand: Direct

Name: nuoI

Synonym: BCc_104

Alternate gene names: 116515045

Gene position: 119859-120404 (Clockwise)

Preceding gene: 116515044

Following gene: 116515046

Centisome position: 28.79

GC content: 27.11

Gene sequence:

>546_bases
ATGAATATGAAAAATATATTTCTTGGTTTTTTAAGTCAAATTCGTAGTATATTAATGATTTTTAGCAACATTTTTTCTTT
TCGTGAAACTCGTTTATATCCTGATGAACCTTTAAATTTATCATTAAGATATCGTGGTCGCATTATTTTAACTCGTAATT
CAAATGGTTCCGAACGTTGTGTTGCATGCAATCTTTGTTCCGCGGTATGCCCGGTAAATTGTATTTCATTAAAAAAATCA
GAAGAAAAGAATGGTCGTTGGTATGCTAAATCTTTTCAAATAAATTTGTCTAGATGTATTTTTTGTGGATTATGTGAGGA
AGCTTGTCCAACTATGGCGATACAATTGACTCCTGATATAGAATTAAGTGAATTTAAAAGAAAAGAATTGTTATATAAAA
AAAAAAATTTACTATTTTCTGGTCCGGGAAAATTTTCAAAATATGATTTTTATTCTATTTCAGGGGTTAATACAACGTAT
GATTTAAAAAAAAATCTTTCAAAAAAAATATCAAATTGTGTTGATGTAACTGATTTATTACCATAA

Upstream 100 bases:

>100_bases
TGTTATGTTATTTGCATGGAAATTTTTTTTTCCTTTATCATTATTAAATATAATTTATACATCTTTTAAAATTTTATTAA
AATAGGAAATACATAAATTT

Downstream 100 bases:

>100_bases
TTAAGGATAATTTTTCATGGCAATCTCTTTTTGGTTATTTTCATTTTTAGCAGTTTTTTTTTCTATTTTAATAATGATTA
GTATAAATTTAATTTATTCT

Product: NADH dehydrogenase I chain I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MNMKNIFLGFLSQIRSILMIFSNIFSFRETRLYPDEPLNLSLRYRGRIILTRNSNGSERCVACNLCSAVCPVNCISLKKS
EEKNGRWYAKSFQINLSRCIFCGLCEEACPTMAIQLTPDIELSEFKRKELLYKKKNLLFSGPGKFSKYDFYSISGVNTTY
DLKKNLSKKISNCVDVTDLLP

Sequences:

>Translated_181_residues
MNMKNIFLGFLSQIRSILMIFSNIFSFRETRLYPDEPLNLSLRYRGRIILTRNSNGSERCVACNLCSAVCPVNCISLKKS
EEKNGRWYAKSFQINLSRCIFCGLCEEACPTMAIQLTPDIELSEFKRKELLYKKKNLLFSGPGKFSKYDFYSISGVNTTY
DLKKNLSKKISNCVDVTDLLP
>Mature_181_residues
MNMKNIFLGFLSQIRSILMIFSNIFSFRETRLYPDEPLNLSLRYRGRIILTRNSNGSERCVACNLCSAVCPVNCISLKKS
EEKNGRWYAKSFQINLSRCIFCGLCEEACPTMAIQLTPDIELSEFKRKELLYKKKNLLFSGPGKFSKYDFYSISGVNTTY
DLKKNLSKKISNCVDVTDLLP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=147, Percent_Identity=37.4149659863946, Blast_Score=105, Evalue=3e-23,
Organism=Escherichia coli, GI1788617, Length=181, Percent_Identity=56.353591160221, Blast_Score=229, Evalue=7e-62,
Organism=Escherichia coli, GI1789075, Length=106, Percent_Identity=32.0754716981132, Blast_Score=68, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17555194, Length=140, Percent_Identity=37.8571428571429, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17864306, Length=137, Percent_Identity=39.4160583941606, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI_BUCCC (Q057W8)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802674.1
- ProteinModelPortal:   Q057W8
- SMR:   Q057W8
- STRING:   Q057W8
- EnsemblBacteria:   EBBUCT00000001458
- GeneID:   4440814
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_104
- eggNOG:   COG1143
- GeneTree:   EBGT00050000007927
- HOGENOM:   HBG724359
- OMA:   DFFRINF
- BioCyc:   BAPH372461:BCC_104-MONOMER
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 20777; Mature: 20777

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
5.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMKNIFLGFLSQIRSILMIFSNIFSFRETRLYPDEPLNLSLRYRGRIILTRNSNGSERC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEEEECCCCCCCE
VACNLCSAVCPVNCISLKKSEEKNGRWYAKSFQINLSRCIFCGLCEEACPTMAIQLTPDI
EEEHHHHHHCCCCEEECCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHCCCEEEEEECCCC
ELSEFKRKELLYKKKNLLFSGPGKFSKYDFYSISGVNTTYDLKKNLSKKISNCVDVTDLL
CHHHHHHHHHHHHHHCCEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
P
C
>Mature Secondary Structure
MNMKNIFLGFLSQIRSILMIFSNIFSFRETRLYPDEPLNLSLRYRGRIILTRNSNGSERC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEEEECCCCCCCE
VACNLCSAVCPVNCISLKKSEEKNGRWYAKSFQINLSRCIFCGLCEEACPTMAIQLTPDI
EEEHHHHHHCCCCEEECCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHCCCEEEEEECCCC
ELSEFKRKELLYKKKNLLFSGPGKFSKYDFYSISGVNTTYDLKKNLSKKISNCVDVTDLL
CHHHHHHHHHHHHHHCCEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA