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The map label for this gene is nuoB

Identifier: 116515039

GI number: 116515039

Start: 111618

End: 112295

Strand: Direct

Name: nuoB

Synonym: BCc_098

Alternate gene names: 116515039

Gene position: 111618-112295 (Clockwise)

Preceding gene: 116515038

Following gene: 116515040

Centisome position: 26.81

GC content: 28.61

Gene sequence:

>678_bases
ATGAAATATACTTTAACTCGTGTTAATACAAAAAATAGTATTTCGAAAAAATATCCTTTACGTTCTTTAAAAAAAACATC
AGATCCAATAAAAAAACAAATAAAAAATAATATTTTTTTTGGAACAATATCAAAATATATGCAATATATTATGAATTGGG
GAAGAAAGAATTCTTTATGGCCGTATAATTTTGGTTTATCTTGTTGTTATGTTGAAATGGTTAGTGCTTTTACATCTATT
CATGATATTTCTCGTTTTGGTTCCGAAGTTTTACGGACCTCTCCTAGACAAGCAGATTTTATGGTTATTGCTGGTACTCC
ATTTATTAAAATGGCTCCAGTAATACAACGATTATATGATCTAATGTTAGAACCAAAATGGGTTATTTCGATGGGTTCTT
GTGCAAATTCAGGTGGTATGTATGACATATATTCTGTTGTTCAAGGAGTTGATAAATTTCTTCCAGTTGATATTTATATA
CCTGGCTGTCCTCCAAGACCAGAAGCATATATTCATGCAATTACTTTATTACAAAAAGCTATTTCTAAAGAACGCAGACC
ATTATCTTGGATAGTAGGTGAGCAAGGGGTGTATAAATATAATATGTTATCTGAGAAAGAAGAAAAAAATAAAAAAAGAA
TTTCTATTATTAATATTGATTCAGAAGATTCTTTTTGA

Upstream 100 bases:

>100_bases
GATAAATTTAAAATTGATAAATAATTTTATAAATTTTTTTATAAATAAATTTATTTATTAAATACAAGAATATAAAAAAA
TAATCATAGGATTTTTTAAA

Downstream 100 bases:

>100_bases
TTTTGTAAAAAAAATTGTTTTGTTTTTTATAAATATATATTTTATATATATAACTATTATATTTTGATTATATTGCTTAT
TATAAGGATAATAAATATGT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MKYTLTRVNTKNSISKKYPLRSLKKTSDPIKKQIKNNIFFGTISKYMQYIMNWGRKNSLWPYNFGLSCCYVEMVSAFTSI
HDISRFGSEVLRTSPRQADFMVIAGTPFIKMAPVIQRLYDLMLEPKWVISMGSCANSGGMYDIYSVVQGVDKFLPVDIYI
PGCPPRPEAYIHAITLLQKAISKERRPLSWIVGEQGVYKYNMLSEKEEKNKKRISIINIDSEDSF

Sequences:

>Translated_225_residues
MKYTLTRVNTKNSISKKYPLRSLKKTSDPIKKQIKNNIFFGTISKYMQYIMNWGRKNSLWPYNFGLSCCYVEMVSAFTSI
HDISRFGSEVLRTSPRQADFMVIAGTPFIKMAPVIQRLYDLMLEPKWVISMGSCANSGGMYDIYSVVQGVDKFLPVDIYI
PGCPPRPEAYIHAITLLQKAISKERRPLSWIVGEQGVYKYNMLSEKEEKNKKRISIINIDSEDSF
>Mature_225_residues
MKYTLTRVNTKNSISKKYPLRSLKKTSDPIKKQIKNNIFFGTISKYMQYIMNWGRKNSLWPYNFGLSCCYVEMVSAFTSI
HDISRFGSEVLRTSPRQADFMVIAGTPFIKMAPVIQRLYDLMLEPKWVISMGSCANSGGMYDIYSVVQGVDKFLPVDIYI
PGCPPRPEAYIHAITLLQKAISKERRPLSWIVGEQGVYKYNMLSEKEEKNKKRISIINIDSEDSF

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=142, Percent_Identity=54.2253521126761, Blast_Score=167, Evalue=7e-42,
Organism=Escherichia coli, GI1788624, Length=225, Percent_Identity=64.8888888888889, Blast_Score=332, Evalue=1e-92,
Organism=Escherichia coli, GI1789074, Length=122, Percent_Identity=39.344262295082, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=39.344262295082, Blast_Score=90, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=142, Percent_Identity=50.7042253521127, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI18859983, Length=142, Percent_Identity=52.112676056338, Blast_Score=165, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24642371, Length=142, Percent_Identity=52.112676056338, Blast_Score=165, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24651058, Length=142, Percent_Identity=54.2253521126761, Blast_Score=165, Evalue=2e-41,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_BUCCC (Q057X4)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802668.1
- ProteinModelPortal:   Q057X4
- SMR:   Q057X4
- STRING:   Q057X4
- EnsemblBacteria:   EBBUCT00000001258
- GeneID:   4440808
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_098
- eggNOG:   COG0377
- GeneTree:   EBGT00050000008096
- HOGENOM:   HBG553221
- OMA:   PRPEAYI
- ProtClustDB:   PRK06411
- BioCyc:   BAPH372461:BCC_098-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 25914; Mature: 25914

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYTLTRVNTKNSISKKYPLRSLKKTSDPIKKQIKNNIFFGTISKYMQYIMNWGRKNSLW
CCEEEEECCCCHHHHHHCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
PYNFGLSCCYVEMVSAFTSIHDISRFGSEVLRTSPRQADFMVIAGTPFIKMAPVIQRLYD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHH
LMLEPKWVISMGSCANSGGMYDIYSVVQGVDKFLPVDIYIPGCPPRPEAYIHAITLLQKA
HHCCCHHEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHH
ISKERRPLSWIVGEQGVYKYNMLSEKEEKNKKRISIINIDSEDSF
HHHCCCCCEEEECCCCCEEEHHHHHHHHCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MKYTLTRVNTKNSISKKYPLRSLKKTSDPIKKQIKNNIFFGTISKYMQYIMNWGRKNSLW
CCEEEEECCCCHHHHHHCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
PYNFGLSCCYVEMVSAFTSIHDISRFGSEVLRTSPRQADFMVIAGTPFIKMAPVIQRLYD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHH
LMLEPKWVISMGSCANSGGMYDIYSVVQGVDKFLPVDIYIPGCPPRPEAYIHAITLLQKA
HHCCCHHEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHH
ISKERRPLSWIVGEQGVYKYNMLSEKEEKNKKRISIINIDSEDSF
HHHCCCCCEEEECCCCCEEEHHHHHHHHCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA