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The map label for this gene is ksgA [H]

Identifier: 116515030

GI number: 116515030

Start: 97688

End: 98515

Strand: Direct

Name: ksgA [H]

Synonym: BCc_089

Alternate gene names: 116515030

Gene position: 97688-98515 (Clockwise)

Preceding gene: 116515029

Following gene: 116515035

Centisome position: 23.46

GC content: 17.87

Gene sequence:

>828_bases
ATGAGATCATTAATATATAAAAATCATGTTCCAATTAAAAAATTAGGACAAAATTTTTTACAGAATAAAGAAATTATTAA
TCAGATAATTAATTTAATAAATATTAATAAAAATGATAATATTATTGAAATAGGATCAGGATTAGGAGCGTTAACTTTTC
CTATTTGTAGAATCATTAAAAAAATGATAGTATTAGAAATTGATGAAGATCTTGTGTTTTTTTTAACTCAAAGTTTATTT
ATTAAAAAATTACAAATTATAATTGCTGATATTATAAAATTTGATTTTTGTTGTTTTTTTTCTTTACAGAAATATAAAAA
ATATAGGTTTATTGGTAATTTACCATATAATATTGCTACTATATTTTTTTTAAAAACAATTAAATTTCTTTATAATATAA
TTGATATGCATTTTATGTTTCAAAAAGAAGTAGCAAAGAGATTATTAGCTACTCCTGGTACTAAAGAATATGGTAGATTA
AGTATTATTGCACAATATTTTTATAAGATAGAAACTGTTATTAATGTTAATAAATTTAATTTTTTTCCTACTCCTAAAGT
AGATTCTACTTTTTTACGATTTACTCCTAAATATTTTAATAGTAAATATAAAATAGATAAACATTTTTCTGTTTTAGAAT
TAATTACTAGATTTTCTTTTCAACATAGAAGAAAATTTTTAAATAATAATTTAATATCTTTATTTTCTACAAAAGAATTA
ATTTCTTTAGATATTGATCCATATTCAAGAGCAGAAAATGTTTCTTTAATTCAATATTGTAAATTAATGAAATATTATTT
GAAAAGAAAAATTTTATGTTTAGATTAA

Upstream 100 bases:

>100_bases
TTATATTTAAAATTTTTAAAATTAGAAATATACATATTTTTAAAAACAAATTTTTTATATAGTTTTTTTTTTTAAAAAAA
TAAATATTTAAAATAATTTT

Downstream 100 bases:

>100_bases
TCAAAATAATTATGATATATTTTTAAATTAATTAACTAATGTATATGTAGTATATTTAAACTTCCTTGTTATTATAATAT
TTTTATATGCGAAATATGTT

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MRSLIYKNHVPIKKLGQNFLQNKEIINQIINLININKNDNIIEIGSGLGALTFPICRIIKKMIVLEIDEDLVFFLTQSLF
IKKLQIIIADIIKFDFCCFFSLQKYKKYRFIGNLPYNIATIFFLKTIKFLYNIIDMHFMFQKEVAKRLLATPGTKEYGRL
SIIAQYFYKIETVINVNKFNFFPTPKVDSTFLRFTPKYFNSKYKIDKHFSVLELITRFSFQHRRKFLNNNLISLFSTKEL
ISLDIDPYSRAENVSLIQYCKLMKYYLKRKILCLD

Sequences:

>Translated_275_residues
MRSLIYKNHVPIKKLGQNFLQNKEIINQIINLININKNDNIIEIGSGLGALTFPICRIIKKMIVLEIDEDLVFFLTQSLF
IKKLQIIIADIIKFDFCCFFSLQKYKKYRFIGNLPYNIATIFFLKTIKFLYNIIDMHFMFQKEVAKRLLATPGTKEYGRL
SIIAQYFYKIETVINVNKFNFFPTPKVDSTFLRFTPKYFNSKYKIDKHFSVLELITRFSFQHRRKFLNNNLISLFSTKEL
ISLDIDPYSRAENVSLIQYCKLMKYYLKRKILCLD
>Mature_275_residues
MRSLIYKNHVPIKKLGQNFLQNKEIINQIINLININKNDNIIEIGSGLGALTFPICRIIKKMIVLEIDEDLVFFLTQSLF
IKKLQIIIADIIKFDFCCFFSLQKYKKYRFIGNLPYNIATIFFLKTIKFLYNIIDMHFMFQKEVAKRLLATPGTKEYGRL
SIIAQYFYKIETVINVNKFNFFPTPKVDSTFLRFTPKYFNSKYKIDKHFSVLELITRFSFQHRRKFLNNNLISLFSTKEL
ISLDIDPYSRAENVSLIQYCKLMKYYLKRKILCLD

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=282, Percent_Identity=30.1418439716312, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI7657198, Length=221, Percent_Identity=33.4841628959276, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1786236, Length=273, Percent_Identity=38.8278388278388, Blast_Score=199, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI25146882, Length=218, Percent_Identity=32.1100917431193, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25141369, Length=285, Percent_Identity=27.719298245614, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6324989, Length=201, Percent_Identity=31.8407960199005, Blast_Score=96, Evalue=8e-21,
Organism=Drosophila melanogaster, GI21358017, Length=219, Percent_Identity=32.8767123287671, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21357273, Length=255, Percent_Identity=25.4901960784314, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 32728; Mature: 32728

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSLIYKNHVPIKKLGQNFLQNKEIINQIINLININKNDNIIEIGSGLGALTFPICRIIK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHH
KMIVLEIDEDLVFFLTQSLFIKKLQIIIADIIKFDFCCFFSLQKYKKYRFIGNLPYNIAT
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IFFLKTIKFLYNIIDMHFMFQKEVAKRLLATPGTKEYGRLSIIAQYFYKIETVINVNKFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEECCCC
FFPTPKVDSTFLRFTPKYFNSKYKIDKHFSVLELITRFSFQHRRKFLNNNLISLFSTKEL
CCCCCCCCCHHHEECHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ISLDIDPYSRAENVSLIQYCKLMKYYLKRKILCLD
HEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRSLIYKNHVPIKKLGQNFLQNKEIINQIINLININKNDNIIEIGSGLGALTFPICRIIK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHH
KMIVLEIDEDLVFFLTQSLFIKKLQIIIADIIKFDFCCFFSLQKYKKYRFIGNLPYNIAT
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IFFLKTIKFLYNIIDMHFMFQKEVAKRLLATPGTKEYGRLSIIAQYFYKIETVINVNKFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEECCCC
FFPTPKVDSTFLRFTPKYFNSKYKIDKHFSVLELITRFSFQHRRKFLNNNLISLFSTKEL
CCCCCCCCCHHHEECHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ISLDIDPYSRAENVSLIQYCKLMKYYLKRKILCLD
HEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]