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The map label for this gene is hisH [H]

Identifier: 116515009

GI number: 116515009

Start: 74556

End: 75146

Strand: Direct

Name: hisH [H]

Synonym: BCc_067

Alternate gene names: 116515009

Gene position: 74556-75146 (Clockwise)

Preceding gene: 116515008

Following gene: 116515010

Centisome position: 17.91

GC content: 20.98

Gene sequence:

>591_bases
ATGTCTATTGTAATTATTAATACTGGTTGTTCTAATCTTTTTTCACTGTATAGTTCTATTAAAAGATTAGGTTATAATGT
TAATGTTACTGATTCAATATGTGATATTAAAAAAGCTAAAAAAATATTTTTACCAGGTGTAGGTACAGCAGCTTATATTC
TTTATATTTTAAAAAAAAAAAAAATATTTTCACTTATAAAATATACTAATCAGCCTATTTTAGGTATTTGTTTAGGTATG
CAATTATTAGGTAAATATAGTTATGAGGGAGGTAAATATTCTTTATTAAATAAGCTTTCTTGTTCTATTAAAAAATTACC
TTCTAAAGATTATTCAATACCACATATTGGATGGAATACAGTTTGTTATAAAAATTCACATATTTTATTTAAAAATATAG
ATAACAATTCTTATTTTTATTTTTTACATAGTTATTATATGGTAGAAAATAAATATTCAATTTCTTCTTCAACACATAGT
ATTAAATTTTGTTCTTCTATAGCAATAAATAATTTTTTTGGTGTACAATTTCATCCTGAAAAATCAGGAATTCCTGGAGA
ACAATTAATTAAAAATTTTTTAGAGATATAA

Upstream 100 bases:

>100_bases
TCATTGTGCAGAAAGTTTATTTAAAGCATTTGGACGTGCTTTAAAACAAGCTATTTTTTTAGAAGGAACTATTTTACCGA
CATCAAAAGGTTTATTTTAA

Downstream 100 bases:

>100_bases
ATATATGATTATTCCATCATTAGATTTTATTCATGGCAAAATTGTACGATTATATCAGGGAAATTATAATAATAAAATAT
CTTATAAGAAAGATATATTT

Product: hypothetical protein

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MSIVIINTGCSNLFSLYSSIKRLGYNVNVTDSICDIKKAKKIFLPGVGTAAYILYILKKKKIFSLIKYTNQPILGICLGM
QLLGKYSYEGGKYSLLNKLSCSIKKLPSKDYSIPHIGWNTVCYKNSHILFKNIDNNSYFYFLHSYYMVENKYSISSSTHS
IKFCSSIAINNFFGVQFHPEKSGIPGEQLIKNFLEI

Sequences:

>Translated_196_residues
MSIVIINTGCSNLFSLYSSIKRLGYNVNVTDSICDIKKAKKIFLPGVGTAAYILYILKKKKIFSLIKYTNQPILGICLGM
QLLGKYSYEGGKYSLLNKLSCSIKKLPSKDYSIPHIGWNTVCYKNSHILFKNIDNNSYFYFLHSYYMVENKYSISSSTHS
IKFCSSIAINNFFGVQFHPEKSGIPGEQLIKNFLEI
>Mature_195_residues
SIVIINTGCSNLFSLYSSIKRLGYNVNVTDSICDIKKAKKIFLPGVGTAAYILYILKKKKIFSLIKYTNQPILGICLGMQ
LLGKYSYEGGKYSLLNKLSCSIKKLPSKDYSIPHIGWNTVCYKNSHILFKNIDNNSYFYFLHSYYMVENKYSISSSTHSI
KFCSSIAINNFFGVQFHPEKSGIPGEQLIKNFLEI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=196, Percent_Identity=43.3673469387755, Blast_Score=185, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6319725, Length=211, Percent_Identity=31.7535545023697, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22253; Mature: 22122

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIVIINTGCSNLFSLYSSIKRLGYNVNVTDSICDIKKAKKIFLPGVGTAAYILYILKKK
CEEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEECCCCCHHHHHHHHHHHH
KIFSLIKYTNQPILGICLGMQLLGKYSYEGGKYSLLNKLSCSIKKLPSKDYSIPHIGWNT
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCE
VCYKNSHILFKNIDNNSYFYFLHSYYMVENKYSISSSTHSIKFCSSIAINNFFGVQFHPE
EEEECCEEEEEECCCCEEEEEEEEEEEEECCEECCCCCCHHHHHHHHHHCCEECEEEECC
KSGIPGEQLIKNFLEI
CCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SIVIINTGCSNLFSLYSSIKRLGYNVNVTDSICDIKKAKKIFLPGVGTAAYILYILKKK
EEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEECCCCCHHHHHHHHHHHH
KIFSLIKYTNQPILGICLGMQLLGKYSYEGGKYSLLNKLSCSIKKLPSKDYSIPHIGWNT
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCE
VCYKNSHILFKNIDNNSYFYFLHSYYMVENKYSISSSTHSIKFCSSIAINNFFGVQFHPE
EEEECCEEEEEECCCCEEEEEEEEEEEEECCEECCCCCCHHHHHHHHHHCCEECEEEECC
KSGIPGEQLIKNFLEI
CCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]