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The map label for this gene is danG [H]

Identifier: 116514980

GI number: 116514980

Start: 39462

End: 41234

Strand: Reverse

Name: danG [H]

Synonym: BCc_035

Alternate gene names: 116514980

Gene position: 41234-39462 (Counterclockwise)

Preceding gene: 116514981

Following gene: 116514979

Centisome position: 9.9

GC content: 16.75

Gene sequence:

>1773_bases
ATGAATACAAAAAAAAAAAAAAAAATTCCTCAAGAATTTATTCATGAAATTATTGAAAAAACAAATATTATTGAAATAAT
TAATAGATACATCACTTTAAAAAAAACTGGAAATAATTATAAAACTTTATGTCCGTTTCATTATGAAAAAACACCTTCAT
TTACTGTAAATTCAGAAAAACAATTTTTTTATTGTTTCGGATGTGGTATACATGGTAATGTGATAGATTTTCTAATGCAA
TATGAAAAATTAGATTTTTTAAGTAGTATCAATGAATTAACAAGTTTACATGGAATAAAAATTCCATATATACAATATAA
TTCTCAATTTAAAAAAAAGTTTTTTTATAAAAAAAAAATTTATAAAATTTTAAAAAAAACATCAAAAATTTATATAAAAA
ATTTATTTAAAGTACCAAATCAAGCATATAACTATCTTATTAAACGTGGCATTACATCAAAAATAATGAAAAATTTTTCA
TTAGGATATGCATTATCAAATAATTATCAAATTACAAATTTTATAAAAAAAAAAACTACTAATATATCTACTATCATTGA
TTGTGGTTTAAGTATATATGAAAAAAACAAAATAAAACAAGATCGATTTAAAGAAAGAATAATATTTCCAATTAAAAATA
AATATGGCATTATAAAAGGTTTCGGAGGAAGAATTATCAATCAATATAACTACCCAAAATATTTAAATTCTCCTGAAACT
ATTACATTTTTTAAAAAAAAAAATTTATATGGAATATATGAATTAATTTTAAAAAATCCTAAACCAAAAAAAATATTAGT
TGTAGAAGGATATTTTGATGTTATTTCACTAGCTCAAAAAAATATTAATTATTCTATTGCATTATTAGGAACTAATATTA
CTACATATCAAATAAAAAAAATTTTTCAAATTAGTAAAAAAATTATATTTTGTTTTGATGGAGATAAAGCAGGTAGAATA
GCATCTTGGAAATCATTGAATTTAATATTACCTTTCTTATATGATGAATATATTATAAATTTTCTTTTGTTACCTAAAAA
TGAAGATCCTAGTAGTTTAATTATTAAAGAAGGAAAAAAAAAATTTGAAAAAAGAATAAAAAAATCAGAATCATTTTATT
CATTTTTTTTTAAAAAAGTTACTAATAAAATAAATTTAAGTTGTATTAATGACTGCATTAAATTAAGTCGAATAACAATC
CCGCTAATTAAAAAAATTCCAAGTAAAATAATAAAAATTTATTTAAGAAAAATTTTAGGTAATAAAATAGGAATATTAGA
TTCTTATCAATTAAAAAAACTCATTCAATTTCAGAAAGAAAAAAAAAAAAAAATCAATAAACCTGTTAAAATTACTACTA
TGCGATTATTAATTAGTTTAATAATACAATATCCTAAATTAGTAAAAAAAATAAAAAACATAAAAAAAATAAAAAAATTA
CATATACAAGGAAAAAAAATACTTATAAAATTAATTGAATTAATTAAAAAAAAAAAAATTTTTAAAACTGGATATTTACT
CGAATTTTTTCGATTTTCTAAATTAGGAAAAATTTTTAAATATTTAAGCACTTGGAATCATATAATTTTAAAAAAAAAAA
TATTTTTTACATTTAAAGAATTTTTAAATAATTTTAAACAACAACATTTAGAATATAAATATAATAAATTAATAACCATA
GAAAGAAAAAAAGGTTTTGATGTAAATGAAAAAAAAAAAATATGGAATATTAGTAAAAAATTAATTTATATTAAAAATAT
AAAATTTAATTAA

Upstream 100 bases:

>100_bases
AAAATCTAAAACGAATTCGAATGTACTAAATAAAAATTTTTTATATTCCAGAATATTAAGACCGTATATTTATATAATAA
CGGTCAAAAAAAAATAAGTT

Downstream 100 bases:

>100_bases
AAAATTTATATTTTATACATTAACATCCATAAAATAAGGTGAATTATTATTTATTTATAAAAAATATACATATTATAATT
TTTAATAAAATTACTTTTAT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MNTKKKKKIPQEFIHEIIEKTNIIEIINRYITLKKTGNNYKTLCPFHYEKTPSFTVNSEKQFFYCFGCGIHGNVIDFLMQ
YEKLDFLSSINELTSLHGIKIPYIQYNSQFKKKFFYKKKIYKILKKTSKIYIKNLFKVPNQAYNYLIKRGITSKIMKNFS
LGYALSNNYQITNFIKKKTTNISTIIDCGLSIYEKNKIKQDRFKERIIFPIKNKYGIIKGFGGRIINQYNYPKYLNSPET
ITFFKKKNLYGIYELILKNPKPKKILVVEGYFDVISLAQKNINYSIALLGTNITTYQIKKIFQISKKIIFCFDGDKAGRI
ASWKSLNLILPFLYDEYIINFLLLPKNEDPSSLIIKEGKKKFEKRIKKSESFYSFFFKKVTNKINLSCINDCIKLSRITI
PLIKKIPSKIIKIYLRKILGNKIGILDSYQLKKLIQFQKEKKKKINKPVKITTMRLLISLIIQYPKLVKKIKNIKKIKKL
HIQGKKILIKLIELIKKKKIFKTGYLLEFFRFSKLGKIFKYLSTWNHIILKKKIFFTFKEFLNNFKQQHLEYKYNKLITI
ERKKGFDVNEKKKIWNISKKLIYIKNIKFN

Sequences:

>Translated_590_residues
MNTKKKKKIPQEFIHEIIEKTNIIEIINRYITLKKTGNNYKTLCPFHYEKTPSFTVNSEKQFFYCFGCGIHGNVIDFLMQ
YEKLDFLSSINELTSLHGIKIPYIQYNSQFKKKFFYKKKIYKILKKTSKIYIKNLFKVPNQAYNYLIKRGITSKIMKNFS
LGYALSNNYQITNFIKKKTTNISTIIDCGLSIYEKNKIKQDRFKERIIFPIKNKYGIIKGFGGRIINQYNYPKYLNSPET
ITFFKKKNLYGIYELILKNPKPKKILVVEGYFDVISLAQKNINYSIALLGTNITTYQIKKIFQISKKIIFCFDGDKAGRI
ASWKSLNLILPFLYDEYIINFLLLPKNEDPSSLIIKEGKKKFEKRIKKSESFYSFFFKKVTNKINLSCINDCIKLSRITI
PLIKKIPSKIIKIYLRKILGNKIGILDSYQLKKLIQFQKEKKKKINKPVKITTMRLLISLIIQYPKLVKKIKNIKKIKKL
HIQGKKILIKLIELIKKKKIFKTGYLLEFFRFSKLGKIFKYLSTWNHIILKKKIFFTFKEFLNNFKQQHLEYKYNKLITI
ERKKGFDVNEKKKIWNISKKLIYIKNIKFN
>Mature_590_residues
MNTKKKKKIPQEFIHEIIEKTNIIEIINRYITLKKTGNNYKTLCPFHYEKTPSFTVNSEKQFFYCFGCGIHGNVIDFLMQ
YEKLDFLSSINELTSLHGIKIPYIQYNSQFKKKFFYKKKIYKILKKTSKIYIKNLFKVPNQAYNYLIKRGITSKIMKNFS
LGYALSNNYQITNFIKKKTTNISTIIDCGLSIYEKNKIKQDRFKERIIFPIKNKYGIIKGFGGRIINQYNYPKYLNSPET
ITFFKKKNLYGIYELILKNPKPKKILVVEGYFDVISLAQKNINYSIALLGTNITTYQIKKIFQISKKIIFCFDGDKAGRI
ASWKSLNLILPFLYDEYIINFLLLPKNEDPSSLIIKEGKKKFEKRIKKSESFYSFFFKKVTNKINLSCINDCIKLSRITI
PLIKKIPSKIIKIYLRKILGNKIGILDSYQLKKLIQFQKEKKKKINKPVKITTMRLLISLIIQYPKLVKKIKNIKKIKKL
HIQGKKILIKLIELIKKKKIFKTGYLLEFFRFSKLGKIFKYLSTWNHIILKKKIFFTFKEFLNNFKQQHLEYKYNKLITI
ERKKGFDVNEKKKIWNISKKLIYIKNIKFN

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=578, Percent_Identity=37.19723183391, Blast_Score=474, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 70230; Mature: 70230

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKKKKKIPQEFIHEIIEKTNIIEIINRYITLKKTGNNYKTLCPFHYEKTPSFTVNSEK
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEECCCCCCCCCCEEECCCC
QFFYCFGCGIHGNVIDFLMQYEKLDFLSSINELTSLHGIKIPYIQYNSQFKKKFFYKKKI
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
YKILKKTSKIYIKNLFKVPNQAYNYLIKRGITSKIMKNFSLGYALSNNYQITNFIKKKTT
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHCCCCEEECCCEEEHHHHHHHCC
NISTIIDCGLSIYEKNKIKQDRFKERIIFPIKNKYGIIKGFGGRIINQYNYPKYLNSPET
CHHHHHHCCHHHHHHCCCCHHHHHHHEEEEECCCCCEEECCCCHHCCCCCCCCCCCCCCE
ITFFKKKNLYGIYELILKNPKPKKILVVEGYFDVISLAQKNINYSIALLGTNITTYQIKK
EEEEECCCCHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHCCCEEEEEEECCCHHHHHHH
IFQISKKIIFCFDGDKAGRIASWKSLNLILPFLYDEYIINFLLLPKNEDPSSLIIKEGKK
HHHHHCEEEEEECCCCCCCEECCCCHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHH
KFEKRIKKSESFYSFFFKKVTNKINLSCINDCIKLSRITIPLIKKIPSKIIKIYLRKILG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NKIGILDSYQLKKLIQFQKEKKKKINKPVKITTMRLLISLIIQYPKLVKKIKNIKKIKKL
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HIQGKKILIKLIELIKKKKIFKTGYLLEFFRFSKLGKIFKYLSTWNHIILKKKIFFTFKE
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLNNFKQQHLEYKYNKLITIERKKGFDVNEKKKIWNISKKLIYIKNIKFN
HHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHEEEEEEECCCC
>Mature Secondary Structure
MNTKKKKKIPQEFIHEIIEKTNIIEIINRYITLKKTGNNYKTLCPFHYEKTPSFTVNSEK
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEECCCCCCCCCCEEECCCC
QFFYCFGCGIHGNVIDFLMQYEKLDFLSSINELTSLHGIKIPYIQYNSQFKKKFFYKKKI
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
YKILKKTSKIYIKNLFKVPNQAYNYLIKRGITSKIMKNFSLGYALSNNYQITNFIKKKTT
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHCCCCEEECCCEEEHHHHHHHCC
NISTIIDCGLSIYEKNKIKQDRFKERIIFPIKNKYGIIKGFGGRIINQYNYPKYLNSPET
CHHHHHHCCHHHHHHCCCCHHHHHHHEEEEECCCCCEEECCCCHHCCCCCCCCCCCCCCE
ITFFKKKNLYGIYELILKNPKPKKILVVEGYFDVISLAQKNINYSIALLGTNITTYQIKK
EEEEECCCCHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHCCCEEEEEEECCCHHHHHHH
IFQISKKIIFCFDGDKAGRIASWKSLNLILPFLYDEYIINFLLLPKNEDPSSLIIKEGKK
HHHHHCEEEEEECCCCCCCEECCCCHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHH
KFEKRIKKSESFYSFFFKKVTNKINLSCINDCIKLSRITIPLIKKIPSKIIKIYLRKILG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NKIGILDSYQLKKLIQFQKEKKKKINKPVKITTMRLLISLIIQYPKLVKKIKNIKKIKKL
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HIQGKKILIKLIELIKKKKIFKTGYLLEFFRFSKLGKIFKYLSTWNHIILKKKIFFTFKE
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLNNFKQQHLEYKYNKLITIERKKGFDVNEKKKIWNISKKLIYIKNIKFN
HHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]