Click here to switch to the map view.

The map label for this gene is lexA

Identifier: 116494275

GI number: 116494275

Start: 729650

End: 730276

Strand: Direct

Name: lexA

Synonym: LSEI_0730

Alternate gene names: 116494275

Gene position: 729650-730276 (Clockwise)

Preceding gene: 116494274

Following gene: 116494279

Centisome position: 25.2

GC content: 46.09

Gene sequence:

>627_bases
ATGCCAACACAAGCAAGTCAGAAACGTTGGAAACAAATTTTACAATCAATCTATGATGCAATCGAGGATCATGGGTACCC
GCCCACGGTTCGCGAAATCGGCAAATCAGTCGGTCTAAGTTCGTCAAGCACGGTGGCAGCGTACTTGGAGAAATTGTTGG
CGGCGGGACTTATTGCTAAGGATCCAGCGAAGCCGCGGACGCTTGAAGTGACCTCAGCCGGACGTGACTTCATTGGCGTT
CAGGATCACGGTATTCCGATCGTCGGCACAGTTGCTGCAGGGGTCCCGATTACGGCCATCGAAAATATCGATGATTATTT
TCCGGTACCAGACGATCTTCCGTATGCCGCTGACGAATTATTCATGTTGCGAGTTCAAGGTAATTCAATGATCAAAATCG
GCATTTTAGATGGCGACCAGATTATTGTCAAGAAGCAGAATGACGCAGAGAATGGACAGATTGTTGTTGCAATGACTGAG
GAAGATGAAGCCACTGTCAAGCGATTTTATAAAGAAAAGAACGGCATTCGGTTACATCCTGAAAATGACAGTATGGATGA
TATGTTTTTCCCGGATGTAACGATTCTTGGCATCGTTGTGAGTTTATATCGGCCGGCATTAGTTTAA

Upstream 100 bases:

>100_bases
TATCTGTGGACTTTTTTGCTATTTAGGCATAAAATCATTGCAAACGTTCGTTCGCTTTGGTATGCTTTGTGTAATCAATT
AGAACTGAGGCGGTGAGCGG

Downstream 100 bases:

>100_bases
CCATAAGAAGTGGTGAGTGACTAGGCTGGCAACTTAAATCCAATTAAAAATGTACCAACATCAAAAGAGACATCAGCTGT
GATCCCACAAAATATAGGAT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MPTQASQKRWKQILQSIYDAIEDHGYPPTVREIGKSVGLSSSSTVAAYLEKLLAAGLIAKDPAKPRTLEVTSAGRDFIGV
QDHGIPIVGTVAAGVPITAIENIDDYFPVPDDLPYAADELFMLRVQGNSMIKIGILDGDQIIVKKQNDAENGQIVVAMTE
EDEATVKRFYKEKNGIRLHPENDSMDDMFFPDVTILGIVVSLYRPALV

Sequences:

>Translated_208_residues
MPTQASQKRWKQILQSIYDAIEDHGYPPTVREIGKSVGLSSSSTVAAYLEKLLAAGLIAKDPAKPRTLEVTSAGRDFIGV
QDHGIPIVGTVAAGVPITAIENIDDYFPVPDDLPYAADELFMLRVQGNSMIKIGILDGDQIIVKKQNDAENGQIVVAMTE
EDEATVKRFYKEKNGIRLHPENDSMDDMFFPDVTILGIVVSLYRPALV
>Mature_207_residues
PTQASQKRWKQILQSIYDAIEDHGYPPTVREIGKSVGLSSSSTVAAYLEKLLAAGLIAKDPAKPRTLEVTSAGRDFIGVQ
DHGIPIVGTVAAGVPITAIENIDDYFPVPDDLPYAADELFMLRVQGNSMIKIGILDGDQIIVKKQNDAENGQIVVAMTEE
DEATVKRFYKEKNGIRLHPENDSMDDMFFPDVTILGIVVSLYRPALV

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=198, Percent_Identity=36.3636363636364, Blast_Score=111, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_LACC3 (Q03B55)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806009.1
- ProteinModelPortal:   Q03B55
- SMR:   Q03B55
- STRING:   Q03B55
- MEROPS:   S24.001
- GeneID:   4421512
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_0730
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- PhylomeDB:   Q03B55
- ProtClustDB:   PRK00215
- BioCyc:   LCAS321967:LSEI_0730-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 22786; Mature: 22655

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: ACT_SITE 129-129 ACT_SITE 167-167

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTQASQKRWKQILQSIYDAIEDHGYPPTVREIGKSVGLSSSSTVAAYLEKLLAAGLIAK
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC
DPAKPRTLEVTSAGRDFIGVQDHGIPIVGTVAAGVPITAIENIDDYFPVPDDLPYAADEL
CCCCCCEEEEECCCCCEECCCCCCCCEEEEHHCCCCEEEECCCHHCCCCCCCCCCCCCEE
FMLRVQGNSMIKIGILDGDQIIVKKQNDAENGQIVVAMTEEDEATVKRFYKEKNGIRLHP
EEEEECCCCEEEEEEECCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEECC
ENDSMDDMFFPDVTILGIVVSLYRPALV
CCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PTQASQKRWKQILQSIYDAIEDHGYPPTVREIGKSVGLSSSSTVAAYLEKLLAAGLIAK
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC
DPAKPRTLEVTSAGRDFIGVQDHGIPIVGTVAAGVPITAIENIDDYFPVPDDLPYAADEL
CCCCCCEEEEECCCCCEECCCCCCCCEEEEHHCCCCEEEECCCHHCCCCCCCCCCCCCEE
FMLRVQGNSMIKIGILDGDQIIVKKQNDAENGQIVVAMTEEDEATVKRFYKEKNGIRLHP
EEEEECCCCEEEEEEECCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEECC
ENDSMDDMFFPDVTILGIVVSLYRPALV
CCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA