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The map label for this gene is sbcD [H]

Identifier: 116494231

GI number: 116494231

Start: 684148

End: 685269

Strand: Direct

Name: sbcD [H]

Synonym: LSEI_0683

Alternate gene names: 116494231

Gene position: 684148-685269 (Clockwise)

Preceding gene: 116494222

Following gene: 116494232

Centisome position: 23.63

GC content: 46.52

Gene sequence:

>1122_bases
ATGAGATTCTTACACACAGCCGATTGGCATATTGGCAAAAAACTAAATGATTTTGATTTGCTTGAGGATCAACAAGCTGT
GTTTGAACAGCTGGTGGAAACGGCTGAGACGCATAAAGTTGATGCCATTGTCATTGCGGGGGATCTCTATGACCGCGCCT
TGCCAAGCGAAGCTGCTGTTTCCGTTCTGGATAACATGTTGATTAAGTTGAATCGTGACCACGGCTATCCGTTGCTGGTC
ATTTCAGGCAACCATGACTCGGCGGTACGCCTGCGCACAGGTCGGGCATGGTATGCCGCTACTAAAATGTTTGTTAATAC
CCAAGTAGCCGAAGCTTTTACACCCATTGAGTTGGATGGCGTGCAATTCTTTTTGCTGCCGTATTTTGAACCCTTTGCTG
TTCGTGACTATTTTCAAGATAAGACAATCACGAATGTGGCTCAGGCAATTCGCCCGATTGTCGCGAAAATGAAGACTTTA
TTTAAGCCAGATATGCGTCATATTCTGGTTAGTCATTTTTTCGCTGCTGGAAGTGACCATAGTGCTTCGGAAACGAAAGT
TAATGTTGGTGGACTGGACGCGGTACCGCTGGATGATTTGGCAGCATTTGATTATGTGGCACTAGGGCATCTGCACAACC
ATCATGCTTTGCATGGTGAACCAAAGATTCAATATAGCGGTGCTTTGTTAAAATACGCGGTCAGCGAGGCCCATCAAGAA
AAAGGGGTCTATATTGTCGATACTGAGACCATGGATCGTGAGTTTGTGCCGTTGAAGCCTAAGCACGATTTAATCGAGTT
AAAAGCTAGTTATGCCGACTTGACGGCTCCAGCATACTATCAGCAGCAGGATCGAGACGCCTATATTGGGATTGATTTGA
CCGATACGCAAGTAATTCCGAATGTCATGGCGCAATTACGCCAGATTTATCCGCGGATTATTAGCCTGCGCCGTGAGAAC
GGCGTGAATGCCATCAAGCCTTTGCAAGAGCGGCAGCGTGATTTGGATCCGATGAGTTTGCTGCAGACTTTTTTTAAAGA
TATGACACAAGATGATTTGTCGGCCGCCCAGATTAAGTGGGCGAAGGCAGGGTTGACGACGGCTAAAAAGGAGCAGGCAT
AA

Upstream 100 bases:

>100_bases
CGAGCAGCGTACCATCGATGTCGAAGATAAAAGTGTTCATGATATTCCTCCCAATAAAGATAATTGTAGCAAGTAAGCGA
TAACAAGGAAAGGCATAACG

Downstream 100 bases:

>100_bases
TGCAACTTAAACAATTACACATGCAATTTTTTGGTCCGTATGCAGATGCAGTCGTTGATTTTGACGATTTTCGCACCAGT
CCCTTATTCTTGATCAGCGG

Product: DNA repair exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MRFLHTADWHIGKKLNDFDLLEDQQAVFEQLVETAETHKVDAIVIAGDLYDRALPSEAAVSVLDNMLIKLNRDHGYPLLV
ISGNHDSAVRLRTGRAWYAATKMFVNTQVAEAFTPIELDGVQFFLLPYFEPFAVRDYFQDKTITNVAQAIRPIVAKMKTL
FKPDMRHILVSHFFAAGSDHSASETKVNVGGLDAVPLDDLAAFDYVALGHLHNHHALHGEPKIQYSGALLKYAVSEAHQE
KGVYIVDTETMDREFVPLKPKHDLIELKASYADLTAPAYYQQQDRDAYIGIDLTDTQVIPNVMAQLRQIYPRIISLRREN
GVNAIKPLQERQRDLDPMSLLQTFFKDMTQDDLSAAQIKWAKAGLTTAKKEQA

Sequences:

>Translated_373_residues
MRFLHTADWHIGKKLNDFDLLEDQQAVFEQLVETAETHKVDAIVIAGDLYDRALPSEAAVSVLDNMLIKLNRDHGYPLLV
ISGNHDSAVRLRTGRAWYAATKMFVNTQVAEAFTPIELDGVQFFLLPYFEPFAVRDYFQDKTITNVAQAIRPIVAKMKTL
FKPDMRHILVSHFFAAGSDHSASETKVNVGGLDAVPLDDLAAFDYVALGHLHNHHALHGEPKIQYSGALLKYAVSEAHQE
KGVYIVDTETMDREFVPLKPKHDLIELKASYADLTAPAYYQQQDRDAYIGIDLTDTQVIPNVMAQLRQIYPRIISLRREN
GVNAIKPLQERQRDLDPMSLLQTFFKDMTQDDLSAAQIKWAKAGLTTAKKEQA
>Mature_373_residues
MRFLHTADWHIGKKLNDFDLLEDQQAVFEQLVETAETHKVDAIVIAGDLYDRALPSEAAVSVLDNMLIKLNRDHGYPLLV
ISGNHDSAVRLRTGRAWYAATKMFVNTQVAEAFTPIELDGVQFFLLPYFEPFAVRDYFQDKTITNVAQAIRPIVAKMKTL
FKPDMRHILVSHFFAAGSDHSASETKVNVGGLDAVPLDDLAAFDYVALGHLHNHHALHGEPKIQYSGALLKYAVSEAHQE
KGVYIVDTETMDREFVPLKPKHDLIELKASYADLTAPAYYQQQDRDAYIGIDLTDTQVIPNVMAQLRQIYPRIISLRREN
GVNAIKPLQERQRDLDPMSLLQTFFKDMTQDDLSAAQIKWAKAGLTTAKKEQA

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=389, Percent_Identity=25.1928020565553, Blast_Score=118, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 42089; Mature: 42089

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLHTADWHIGKKLNDFDLLEDQQAVFEQLVETAETHKVDAIVIAGDLYDRALPSEAAV
CCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHCCHHHHH
SVLDNMLIKLNRDHGYPLLVISGNHDSAVRLRTGRAWYAATKMFVNTQVAEAFTPIELDG
HHHHHHHEEEECCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEECC
VQFFLLPYFEPFAVRDYFQDKTITNVAQAIRPIVAKMKTLFKPDMRHILVSHFFAAGSDH
EEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC
SASETKVNVGGLDAVPLDDLAAFDYVALGHLHNHHALHGEPKIQYSGALLKYAVSEAHQE
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHH
KGVYIVDTETMDREFVPLKPKHDLIELKASYADLTAPAYYQQQDRDAYIGIDLTDTQVIP
CCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCEEEEEECCCHHHHH
NVMAQLRQIYPRIISLRRENGVNAIKPLQERQRDLDPMSLLQTFFKDMTQDDLSAAQIKW
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHH
AKAGLTTAKKEQA
HHHCCCCHHCCCC
>Mature Secondary Structure
MRFLHTADWHIGKKLNDFDLLEDQQAVFEQLVETAETHKVDAIVIAGDLYDRALPSEAAV
CCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHCCHHHHH
SVLDNMLIKLNRDHGYPLLVISGNHDSAVRLRTGRAWYAATKMFVNTQVAEAFTPIELDG
HHHHHHHEEEECCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEECC
VQFFLLPYFEPFAVRDYFQDKTITNVAQAIRPIVAKMKTLFKPDMRHILVSHFFAAGSDH
EEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC
SASETKVNVGGLDAVPLDDLAAFDYVALGHLHNHHALHGEPKIQYSGALLKYAVSEAHQE
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHH
KGVYIVDTETMDREFVPLKPKHDLIELKASYADLTAPAYYQQQDRDAYIGIDLTDTQVIP
CCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCEEEEEECCCHHHHH
NVMAQLRQIYPRIISLRRENGVNAIKPLQERQRDLDPMSLLQTFFKDMTQDDLSAAQIKW
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHH
AKAGLTTAKKEQA
HHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA