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The map label for this gene is apbE [H]

Identifier: 116494145

GI number: 116494145

Start: 581748

End: 582665

Strand: Direct

Name: apbE [H]

Synonym: LSEI_0592

Alternate gene names: 116494145

Gene position: 581748-582665 (Clockwise)

Preceding gene: 116494137

Following gene: 116494146

Centisome position: 20.09

GC content: 39.87

Gene sequence:

>918_bases
ATGCTAACTTGGCGAGTGAAGCGATTACGAGCTTTAGGAACAAATATAGTTTTGTCGGTATTTGGCGAGCAAGATAATTC
TGCATTAGTTGCTGCCGAATTGCTAATCAGGCAACAAGAATCTAGGTTGACAGTAAATCAACCTCATTCAGAAATAATGA
CCATAAATCAACTAGCAGGATATGACAATACTGAGATTTCGTGTGGTACGTATGCACTTATAAAAAATGCTGTCCTAGCA
AGCCAAAAGCACTTTGGATTCAATGCCTTGATTGGACCACTTGTCAAACTTTGGAAGATAGGTTTCAACGGTGCCAATGT
TCCATCCGATCACGACATTAAGTCTCGGCTACTTTTAAGCAATCCCGATAACATTATTATGGATGATGCGTTGCACACCG
TAAAACTAGTCAAAACTGGTATGGAGCTCGATCTTGGAGGAATTGCAAAGGGATTCATTGCTGATCAAATTCGGTCTATT
TGGTTTGCCTATGGGATTAGGGCTGGAATAATTAATCTCGGTGGTAATTTACTATTTGTTGGAGAATCTCCACGACGATC
TGATGGCCGATGGATAGTTGGTATTCAGGATCCCAATAAAGGAAGAAATATTGGCATTGGAACGGTCATTAAACCTGCAT
GTGCAGCTGTTACAAGTGGTATCTACGAACGATTTTTAATAAAAGGGGGTAAAAGATATCATCATTTGTTGGATCCTCAA
ACTGGATATCCTTTACAAACCCAATTGGCTGCTGTTACGGTATTTGCTCGTGATTCTCTGCAGGGTGAATTAGAAGCCAA
GCGATTATTTTTTGCTGGTAAGCCTATTCCAGGATGGGAGAAAGATCCTAATAATTTAGGCGCTGTTTTTATCTATCAAA
ATGGCAATACACAAACGGTCAAGGCCTCAATTCATTAA

Upstream 100 bases:

>100_bases
GGAATTCCATAAAAAAACACCTTCGGTCGTACTACAACCAGAAGAGGGAAATCATGAGAAAAGTGATTCAAAATACAGTA
TTTATAGAAATGATGATACA

Downstream 100 bases:

>100_bases
ATCTCCTTAACTATAAGTTATCAGGTTCACTGCCGTTCTCTAAGAGTGACAAGATTGCTACTCTGCAAGCTAAAAAGGAA
TATAATTATCCTGGGTAAGG

Product: thiamine biosynthesis membrane-associated lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MLTWRVKRLRALGTNIVLSVFGEQDNSALVAAELLIRQQESRLTVNQPHSEIMTINQLAGYDNTEISCGTYALIKNAVLA
SQKHFGFNALIGPLVKLWKIGFNGANVPSDHDIKSRLLLSNPDNIIMDDALHTVKLVKTGMELDLGGIAKGFIADQIRSI
WFAYGIRAGIINLGGNLLFVGESPRRSDGRWIVGIQDPNKGRNIGIGTVIKPACAAVTSGIYERFLIKGGKRYHHLLDPQ
TGYPLQTQLAAVTVFARDSLQGELEAKRLFFAGKPIPGWEKDPNNLGAVFIYQNGNTQTVKASIH

Sequences:

>Translated_305_residues
MLTWRVKRLRALGTNIVLSVFGEQDNSALVAAELLIRQQESRLTVNQPHSEIMTINQLAGYDNTEISCGTYALIKNAVLA
SQKHFGFNALIGPLVKLWKIGFNGANVPSDHDIKSRLLLSNPDNIIMDDALHTVKLVKTGMELDLGGIAKGFIADQIRSI
WFAYGIRAGIINLGGNLLFVGESPRRSDGRWIVGIQDPNKGRNIGIGTVIKPACAAVTSGIYERFLIKGGKRYHHLLDPQ
TGYPLQTQLAAVTVFARDSLQGELEAKRLFFAGKPIPGWEKDPNNLGAVFIYQNGNTQTVKASIH
>Mature_305_residues
MLTWRVKRLRALGTNIVLSVFGEQDNSALVAAELLIRQQESRLTVNQPHSEIMTINQLAGYDNTEISCGTYALIKNAVLA
SQKHFGFNALIGPLVKLWKIGFNGANVPSDHDIKSRLLLSNPDNIIMDDALHTVKLVKTGMELDLGGIAKGFIADQIRSI
WFAYGIRAGIINLGGNLLFVGESPRRSDGRWIVGIQDPNKGRNIGIGTVIKPACAAVTSGIYERFLIKGGKRYHHLLDPQ
TGYPLQTQLAAVTVFARDSLQGELEAKRLFFAGKPIPGWEKDPNNLGAVFIYQNGNTQTVKASIH

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=229, Percent_Identity=25.764192139738, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 33441; Mature: 33441

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTWRVKRLRALGTNIVLSVFGEQDNSALVAAELLIRQQESRLTVNQPHSEIMTINQLAG
CCCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEECCCHHHHEEHHHHCC
YDNTEISCGTYALIKNAVLASQKHFGFNALIGPLVKLWKIGFNGANVPSDHDIKSRLLLS
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEC
NPDNIIMDDALHTVKLVKTGMELDLGGIAKGFIADQIRSIWFAYGIRAGIINLGGNLLFV
CCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEE
GESPRRSDGRWIVGIQDPNKGRNIGIGTVIKPACAAVTSGIYERFLIKGGKRYHHLLDPQ
ECCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHCCCCC
TGYPLQTQLAAVTVFARDSLQGELEAKRLFFAGKPIPGWEKDPNNLGAVFIYQNGNTQTV
CCCCHHHHHEEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCEEE
KASIH
EEECC
>Mature Secondary Structure
MLTWRVKRLRALGTNIVLSVFGEQDNSALVAAELLIRQQESRLTVNQPHSEIMTINQLAG
CCCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEECCCHHHHEEHHHHCC
YDNTEISCGTYALIKNAVLASQKHFGFNALIGPLVKLWKIGFNGANVPSDHDIKSRLLLS
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEC
NPDNIIMDDALHTVKLVKTGMELDLGGIAKGFIADQIRSIWFAYGIRAGIINLGGNLLFV
CCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEE
GESPRRSDGRWIVGIQDPNKGRNIGIGTVIKPACAAVTSGIYERFLIKGGKRYHHLLDPQ
ECCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHCCCCC
TGYPLQTQLAAVTVFARDSLQGELEAKRLFFAGKPIPGWEKDPNNLGAVFIYQNGNTQTV
CCCCHHHHHEEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCEEE
KASIH
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]