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The map label for this gene is insI1 [C]

Identifier: 116494125

GI number: 116494125

Start: 562091

End: 562750

Strand: Direct

Name: insI1 [C]

Synonym: LSEI_0571

Alternate gene names: 116494125

Gene position: 562091-562750 (Clockwise)

Preceding gene: 116494124

Following gene: 116494126

Centisome position: 19.41

GC content: 47.12

Gene sequence:

>660_bases
ATGGTCGGTTATGCCGTTCACTGTGCGCCACACACGATTTACCACTGGATTTATCAAAGACAAGTCGATTTTCAGCCAAG
CCAACTCTTTGATCACGGTAAACGTCATAAAAGAAGACAAGACCTTCGGTCGCGCTATAACCAAGCAGTAGGCACCTCAA
TTGAGATTCGCAGTGAGTCAGCTAATCGGCGAACCGAAAAAGGACATTTAGAGATGGATACAGTTCGCGGTGGTCGCGGG
TCAAAGGCTGCTGTTTTGACCATTGTCGATCGGGTGACACGTTTAATGGCGACAACTAAGCTTGAAAACTTATCACAAAA
TGCTGTTCTCAAGGGATTTGCAAGACTGATGGTGGACTTTCCGGGTCCGGTTCGATCAGTGACGGTTGATCACGGTAAAG
AGTTTTCCTGCGATCAGGCGCTTACAAAGCGCTATCGGATACCGGTTTACTTTTGCCACGCCTATCACCCGAATGAACGG
GGCACAAATGAACGGTTCAATCGAGAACTTCGCTACTATTTCCCGAAGGGAACACAGTTTGATCAGGTTTCAGAGACCGA
TATTCAACAAGCCACAGCGCTTATCAATAACAAACCTAGAAAATGTCTCCGTTGGCAAACCCCAGTTCAAGCAGTGAGCA
AGCCTCTTTCTAGGTGGTAA

Upstream 100 bases:

>100_bases
TGCCGATACTTGTCGGCATCGTTGTGGTCGGCACTCGATTTTAACGCCTAAGTTGAAGCGGATGGTAACTGAGAAGCTAA
ACCTAGGTTGGTCCCCTGAA

Downstream 100 bases:

>100_bases
CTTTATTATTGCAATCTAGGAAGATTATTAGATAATTTGCAACCGAACAATGATAAACTAGGACCTTTTTTGGCCCAATT
AACTAACAAGTACCAAATTG

Product: IS30 family transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MVGYAVHCAPHTIYHWIYQRQVDFQPSQLFDHGKRHKRRQDLRSRYNQAVGTSIEIRSESANRRTEKGHLEMDTVRGGRG
SKAAVLTIVDRVTRLMATTKLENLSQNAVLKGFARLMVDFPGPVRSVTVDHGKEFSCDQALTKRYRIPVYFCHAYHPNER
GTNERFNRELRYYFPKGTQFDQVSETDIQQATALINNKPRKCLRWQTPVQAVSKPLSRW

Sequences:

>Translated_219_residues
MVGYAVHCAPHTIYHWIYQRQVDFQPSQLFDHGKRHKRRQDLRSRYNQAVGTSIEIRSESANRRTEKGHLEMDTVRGGRG
SKAAVLTIVDRVTRLMATTKLENLSQNAVLKGFARLMVDFPGPVRSVTVDHGKEFSCDQALTKRYRIPVYFCHAYHPNER
GTNERFNRELRYYFPKGTQFDQVSETDIQQATALINNKPRKCLRWQTPVQAVSKPLSRW
>Mature_219_residues
MVGYAVHCAPHTIYHWIYQRQVDFQPSQLFDHGKRHKRRQDLRSRYNQAVGTSIEIRSESANRRTEKGHLEMDTVRGGRG
SKAAVLTIVDRVTRLMATTKLENLSQNAVLKGFARLMVDFPGPVRSVTVDHGKEFSCDQALTKRYRIPVYFCHAYHPNER
GTNERFNRELRYYFPKGTQFDQVSETDIQQATALINNKPRKCLRWQTPVQAVSKPLSRW

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786450, Length=222, Percent_Identity=31.5315315315315, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1790736, Length=222, Percent_Identity=31.5315315315315, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787671, Length=222, Percent_Identity=31.5315315315315, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 25478; Mature: 25478

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: PS50994 INTEGRASE ; PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGYAVHCAPHTIYHWIYQRQVDFQPSQLFDHGKRHKRRQDLRSRYNQAVGTSIEIRSES
CCCEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCC
ANRRTEKGHLEMDTVRGGRGSKAAVLTIVDRVTRLMATTKLENLSQNAVLKGFARLMVDF
CCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
PGPVRSVTVDHGKEFSCDQALTKRYRIPVYFCHAYHPNERGTNERFNRELRYYFPKGTQF
CCCCEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCHHHCCCCEEECCCCCCC
DQVSETDIQQATALINNKPRKCLRWQTPVQAVSKPLSRW
CCCCHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MVGYAVHCAPHTIYHWIYQRQVDFQPSQLFDHGKRHKRRQDLRSRYNQAVGTSIEIRSES
CCCEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCC
ANRRTEKGHLEMDTVRGGRGSKAAVLTIVDRVTRLMATTKLENLSQNAVLKGFARLMVDF
CCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
PGPVRSVTVDHGKEFSCDQALTKRYRIPVYFCHAYHPNERGTNERFNRELRYYFPKGTQF
CCCCEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCHHHCCCCEEECCCCCCC
DQVSETDIQQATALINNKPRKCLRWQTPVQAVSKPLSRW
CCCCHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8393068 [H]