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The map label for this gene is rlmH

Identifier: 116493557

GI number: 116493557

Start: 1809020

End: 1809499

Strand: Reverse

Name: rlmH

Synonym: PEPE_1831

Alternate gene names: 116493557

Gene position: 1809499-1809020 (Counterclockwise)

Preceding gene: 116493558

Following gene: 116493554

Centisome position: 98.75

GC content: 35.21

Gene sequence:

>480_bases
GTGAATATTAAAATTATTGTTGTGGGAAAGTTAAAAGAAAAATATTTGAAACAGGGGATAGCAGAATACTCGAAAAGATT
GTCAAAATTTTGTAAGTTCCAAATTATTGAAGTTCCAGATGAAAAAGCACCCGAAAGTTTAAGCGATGCTCAAATGAATG
AAGTTAAAGTTAAGGAAGGAGAACGTATTTTAGGTAAAATTAAAGATCGAGACTATGTTTTTACCCTAGAAATCAAAGGA
AAAGAACGCTCTTCTGAAGAATTATCAGCAGAGATGGCTAAATTAGCTACTTATGGAAACTCTGATATCACATTTGTGAT
CGGGGGTTCGTTAGGTCTTAGTGATGCAGTAATGAAACGTTCTAATTCCGCCATTTCATTTGGGCGCTTTACTCTACCGC
ACCAATTGATGCGACTAGTGTTAACTGAGCAAATTTATCGTTCGTTTATGATTCAGGCGGGAAGTCCGTATCATAAATAA

Upstream 100 bases:

>100_bases
AATGTAGTGCCGAACATAAATTCGACCCAATATGTTGTGAGTGCAAAAATTAGCCTGTGGATAACTTTGTTAATAACTTA
AAAAAGGATGTGCAAAAGAA

Downstream 100 bases:

>100_bases
TCAAAAACACTTACCAAGTTTGTCTTGGTAAGTGTTTTTTGTTTAAACATAATTATGAACGGATTTAATGTATTATGATG
TTTTTTTCATCAGTAAGGTT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MNIKIIVVGKLKEKYLKQGIAEYSKRLSKFCKFQIIEVPDEKAPESLSDAQMNEVKVKEGERILGKIKDRDYVFTLEIKG
KERSSEELSAEMAKLATYGNSDITFVIGGSLGLSDAVMKRSNSAISFGRFTLPHQLMRLVLTEQIYRSFMIQAGSPYHK

Sequences:

>Translated_159_residues
MNIKIIVVGKLKEKYLKQGIAEYSKRLSKFCKFQIIEVPDEKAPESLSDAQMNEVKVKEGERILGKIKDRDYVFTLEIKG
KERSSEELSAEMAKLATYGNSDITFVIGGSLGLSDAVMKRSNSAISFGRFTLPHQLMRLVLTEQIYRSFMIQAGSPYHK
>Mature_159_residues
MNIKIIVVGKLKEKYLKQGIAEYSKRLSKFCKFQIIEVPDEKAPESLSDAQMNEVKVKEGERILGKIKDRDYVFTLEIKG
KERSSEELSAEMAKLATYGNSDITFVIGGSLGLSDAVMKRSNSAISFGRFTLPHQLMRLVLTEQIYRSFMIQAGSPYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=159, Percent_Identity=31.4465408805031, Blast_Score=98, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_PEDPA (Q03D72)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_805292.1
- ProteinModelPortal:   Q03D72
- SMR:   Q03D72
- STRING:   Q03D72
- GeneID:   4417388
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1831
- NMPDR:   fig|278197.10.peg.1621
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- PhylomeDB:   Q03D72
- BioCyc:   PPEN278197:PEPE_1831-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 18000; Mature: 18000

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKIIVVGKLKEKYLKQGIAEYSKRLSKFCKFQIIEVPDEKAPESLSDAQMNEVKVKEG
CCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHHEECCCC
ERILGKIKDRDYVFTLEIKGKERSSEELSAEMAKLATYGNSDITFVIGGSLGLSDAVMKR
HHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHC
SNSAISFGRFTLPHQLMRLVLTEQIYRSFMIQAGSPYHK
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNIKIIVVGKLKEKYLKQGIAEYSKRLSKFCKFQIIEVPDEKAPESLSDAQMNEVKVKEG
CCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHHEECCCC
ERILGKIKDRDYVFTLEIKGKERSSEELSAEMAKLATYGNSDITFVIGGSLGLSDAVMKR
HHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHC
SNSAISFGRFTLPHQLMRLVLTEQIYRSFMIQAGSPYHK
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA