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The map label for this gene is manX [H]

Identifier: 116493487

GI number: 116493487

Start: 1733804

End: 1734298

Strand: Reverse

Name: manX [H]

Synonym: PEPE_1759

Alternate gene names: 116493487

Gene position: 1734298-1733804 (Counterclockwise)

Preceding gene: 116493488

Following gene: 116493486

Centisome position: 94.65

GC content: 38.18

Gene sequence:

>495_bases
ATGACAATTTCAGCGGTACGAATTGATGGACGGCTAATCCATGGACAAGTAGCCAATTTGTGGACAACGAAGCTCGGTGT
AACAAGAATTATGGTGATTGATGACAAAGTGGCTGCAAGCGATATTGAAAAATCGGGTTTGAAATTAGCTCGACCAGCTG
GTATTGATTTGAGTGTGCTCTCTGAAAAGGTAGCTGCTGATCATATTAAGCGTGGCGGCTATGATTCGCAAAAAGTATTT
ATTGTGGTTAAACGACCACAAGTATTATTGGACTTGGTTAACGACGGAGTGAAGTTAGAAACGATTAATGTCGGAAATAT
GTCACAGACGGATGAGACGACTCAAATCACTAATTCTATTAATGTAGTGCAAGACGATGTAGATGCATTTAAGGCACTAC
ATGAAAAAGGAATTAAAATCACTCAGCAAATGGTTCCTGGTGATCAAGCCAATGACTTTATGGCAATTTTGAAAAAATTT
GATACTAAAAAATAA

Upstream 100 bases:

>100_bases
ATGGATTGAGCATGATGTATTAACCATCCCATATCAACTAAAAGGCTTAGAAACAGTAATTTTATTAGCATAGTTTTGAG
AAAACAAAGGAGTGAGTAGT

Downstream 100 bases:

>100_bases
GAAGGAGGCGTTCTTATGGGTTCAGGTATTGCAATATGGCAAGTAATTATTTTAACAATTTATTCAATGTACCAGATTAT
GGATGAGTTAACGCTCCAAA

Product: phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MTISAVRIDGRLIHGQVANLWTTKLGVTRIMVIDDKVAASDIEKSGLKLARPAGIDLSVLSEKVAADHIKRGGYDSQKVF
IVVKRPQVLLDLVNDGVKLETINVGNMSQTDETTQITNSINVVQDDVDAFKALHEKGIKITQQMVPGDQANDFMAILKKF
DTKK

Sequences:

>Translated_164_residues
MTISAVRIDGRLIHGQVANLWTTKLGVTRIMVIDDKVAASDIEKSGLKLARPAGIDLSVLSEKVAADHIKRGGYDSQKVF
IVVKRPQVLLDLVNDGVKLETINVGNMSQTDETTQITNSINVVQDDVDAFKALHEKGIKITQQMVPGDQANDFMAILKKF
DTKK
>Mature_163_residues
TISAVRIDGRLIHGQVANLWTTKLGVTRIMVIDDKVAASDIEKSGLKLARPAGIDLSVLSEKVAADHIKRGGYDSQKVFI
VVKRPQVLLDLVNDGVKLETINVGNMSQTDETTQITNSINVVQDDVDAFKALHEKGIKITQQMVPGDQANDFMAILKKFD
TKK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=161, Percent_Identity=34.7826086956522, Blast_Score=99, Evalue=1e-22,
Organism=Escherichia coli, GI87082217, Length=151, Percent_Identity=34.4370860927152, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1789527, Length=148, Percent_Identity=32.4324324324324, Blast_Score=72, Evalue=1e-14,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789
- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17984; Mature: 17853

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISAVRIDGRLIHGQVANLWTTKLGVTRIMVIDDKVAASDIEKSGLKLARPAGIDLSVL
CCEEEEEECCEEEECHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCEEECCCCCCHHHH
SEKVAADHIKRGGYDSQKVFIVVKRPQVLLDLVNDGVKLETINVGNMSQTDETTQITNSI
HHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHH
NVVQDDVDAFKALHEKGIKITQQMVPGDQANDFMAILKKFDTKK
HHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
TISAVRIDGRLIHGQVANLWTTKLGVTRIMVIDDKVAASDIEKSGLKLARPAGIDLSVL
CEEEEEECCEEEECHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCEEECCCCCCHHHH
SEKVAADHIKRGGYDSQKVFIVVKRPQVLLDLVNDGVKLETINVGNMSQTDETTQITNSI
HHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHH
NVVQDDVDAFKALHEKGIKITQQMVPGDQANDFMAILKKFDTKK
HHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]