Click here to switch to the map view.

The map label for this gene is manZ [H]

Identifier: 116493485

GI number: 116493485

Start: 1732063

End: 1732887

Strand: Reverse

Name: manZ [H]

Synonym: PEPE_1757

Alternate gene names: 116493485

Gene position: 1732887-1732063 (Counterclockwise)

Preceding gene: 116493486

Following gene: 116493484

Centisome position: 94.57

GC content: 37.82

Gene sequence:

>825_bases
ATGGCGACGAACTCTAATAAGACTAATTATAAATTAACTGATAAAGACTTTAAGCAAATGAATATCCGTAGCCTCTTATT
CAACCAAGTTGGATGGAATTACGAACGTATGCAAGGATCCGGTTATCTTTACTTGATTCTTCCCCAGCTCCGTAAAATGT
ATGGTGACCATACTCCCGAGCTGAAAAAAATGATGAAAATGCAAAATCAATTCTTTAATACAAGTAACTTCTTTAATACT
ATTATTTCAGGAATTGATATGGCGATTGAAGAAAAAGAAGGTGTTAATTCACTTGAAACCGTTGCGGGAATTAAGGCAGG
TTTAATGGGACCTTTTGCCGCAATTGGTGATTCGATTTTTGCCGCTTTGGTGCCTGCTATTATGGGAGCAATTGCTGCTA
ATATGGCGGTTCAAGGTAACGTTGTAGGTGCTATTTTATGGATGTTAGTTAACTTCGTTATTATGTGGTTCCGCTGGATT
CAACTGAAATTTGCCTACAAACAAGGGGTAAGTTTAGTTAATGAAATGCAAGGACAGCTAAATGCTTTGACGGATGCAGC
AACGTTACTTGGAGTTTATGTCGTAGGTGCTTTAGCAGCTACTATGGTTAATACTCATGTGATTTTCGTAGCACATTTAG
GGAAGATGACCGTTAACTTACAAGATAAGATTGATATGATCATGCCAAAATTGGTCCCTGCTTTAATTGTTGGAGCAATC
TATTGGATGCTTGGTCGAAAGAGAATGACTTCAACAAAAGCTATCTTTATCGTGATTATCGTGGCAGTTGTGCTATCAGC
ACTTGGCATAATCGGTAAAGCTTAA

Upstream 100 bases:

>100_bases
ATGCAGTACAATCGTTCCGTTAAAGAAAGCAATTTAGTAGCTGCACAACCTAAACAAAATGCTGGTGCAACAGATTTAGA
TGAACAGGATGGTGAGATTC

Downstream 100 bases:

>100_bases
CATTAATTGCCACTCAAAACCTAAATTTGGTATATCATATAAATTATGAATCAGATTTAGAAAGTGTGGTAATCAAACCA
TGGGATTTAACTATAATGAA

Product: phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EII-M-Man; EIID-Man; PTS system mannose-specific EIID component [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MATNSNKTNYKLTDKDFKQMNIRSLLFNQVGWNYERMQGSGYLYLILPQLRKMYGDHTPELKKMMKMQNQFFNTSNFFNT
IISGIDMAIEEKEGVNSLETVAGIKAGLMGPFAAIGDSIFAALVPAIMGAIAANMAVQGNVVGAILWMLVNFVIMWFRWI
QLKFAYKQGVSLVNEMQGQLNALTDAATLLGVYVVGALAATMVNTHVIFVAHLGKMTVNLQDKIDMIMPKLVPALIVGAI
YWMLGRKRMTSTKAIFIVIIVAVVLSALGIIGKA

Sequences:

>Translated_274_residues
MATNSNKTNYKLTDKDFKQMNIRSLLFNQVGWNYERMQGSGYLYLILPQLRKMYGDHTPELKKMMKMQNQFFNTSNFFNT
IISGIDMAIEEKEGVNSLETVAGIKAGLMGPFAAIGDSIFAALVPAIMGAIAANMAVQGNVVGAILWMLVNFVIMWFRWI
QLKFAYKQGVSLVNEMQGQLNALTDAATLLGVYVVGALAATMVNTHVIFVAHLGKMTVNLQDKIDMIMPKLVPALIVGAI
YWMLGRKRMTSTKAIFIVIIVAVVLSALGIIGKA
>Mature_273_residues
ATNSNKTNYKLTDKDFKQMNIRSLLFNQVGWNYERMQGSGYLYLILPQLRKMYGDHTPELKKMMKMQNQFFNTSNFFNTI
ISGIDMAIEEKEGVNSLETVAGIKAGLMGPFAAIGDSIFAALVPAIMGAIAANMAVQGNVVGAILWMLVNFVIMWFRWIQ
LKFAYKQGVSLVNEMQGQLNALTDAATLLGVYVVGALAATMVNTHVIFVAHLGKMTVNLQDKIDMIMPKLVPALIVGAIY
WMLGRKRMTSTKAIFIVIIVAVVLSALGIIGKA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1788122, Length=282, Percent_Identity=33.3333333333333, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1789529, Length=263, Percent_Identity=32.319391634981, Blast_Score=136, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: NA

Molecular weight: Translated: 30242; Mature: 30110

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
7.3 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
7.0 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATNSNKTNYKLTDKDFKQMNIRSLLFNQVGWNYERMQGSGYLYLILPQLRKMYGDHTPE
CCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEHHHHHHHHCCCCHH
LKKMMKMQNQFFNTSNFFNTIISGIDMAIEEKEGVNSLETVAGIKAGLMGPFAAIGDSIF
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHH
AALVPAIMGAIAANMAVQGNVVGAILWMLVNFVIMWFRWIQLKFAYKQGVSLVNEMQGQL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NALTDAATLLGVYVVGALAATMVNTHVIFVAHLGKMTVNLQDKIDMIMPKLVPALIVGAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHEECHHHHHHHHHHHHHHHHHHHHH
YWMLGRKRMTSTKAIFIVIIVAVVLSALGIIGKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ATNSNKTNYKLTDKDFKQMNIRSLLFNQVGWNYERMQGSGYLYLILPQLRKMYGDHTPE
CCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEHHHHHHHHCCCCHH
LKKMMKMQNQFFNTSNFFNTIISGIDMAIEEKEGVNSLETVAGIKAGLMGPFAAIGDSIF
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHH
AALVPAIMGAIAANMAVQGNVVGAILWMLVNFVIMWFRWIQLKFAYKQGVSLVNEMQGQL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NALTDAATLLGVYVVGALAATMVNTHVIFVAHLGKMTVNLQDKIDMIMPKLVPALIVGAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHEECHHHHHHHHHHHHHHHHHHHHH
YWMLGRKRMTSTKAIFIVIIVAVVLSALGIIGKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]