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The map label for this gene is ywbI [H]

Identifier: 116493413

GI number: 116493413

Start: 1662416

End: 1663300

Strand: Reverse

Name: ywbI [H]

Synonym: PEPE_1685

Alternate gene names: 116493413

Gene position: 1663300-1662416 (Counterclockwise)

Preceding gene: 116493417

Following gene: 116493412

Centisome position: 90.77

GC content: 39.32

Gene sequence:

>885_bases
ATGGAAATTAGAACTCTGCGTTATTTTTGGACAATTGCAGAAGAAGGAACGGTTTCTAAGGCAGCGGAAGCTTTGCACAT
TACCCAACCTACTTTAAGTCGACAAATCAAAGAATTAGAAGAAGAATTGGGAACGGAGTTATTTATTCGAGGGCGGCGCC
AGGTTCGATTAACAGAAGCAGGAATGTTTCTAAAATCCCGTGCAGAAGAAATTTTAGAGCTAACCCAGCAAACCAGTTTA
GAATTCGAAAACCGTAAAAAGCAACTCTTTAGTGGACATATCACCATCGGATGCATTGAAGCCGATAATTCTGATACTTT
AGCCTTGATTTTAGAGGATTTTATCCGAGATTATCCGCAAGTTACGTTTACCATTTACAGTGGGACTAGTGACGACATCA
CAGAGCGTTTAGATAAGGGCTTAGTGGATGTTGCAGTTTTAATTAAGCCCATCGCCACAGAAAAATATCACGAAATTGTA
CTTCCCCGTACTGAAAAATGGGGATTATTAGTGTCCAAAGCATCGTATATTGCTCAGAAAGATAAAATTGTACCGGCTGA
TTTAATGGGGATGCCGTTGTTAGTGTCAGGGCGTCCTGAAGTTCAGGAGTTAATTTCAAAATGGAGCAAGATTGACTATC
AACAATTAAATGTTGTCGGTCATTATAATTTAACCTTTAATGTGCTCCCACTAGTGAAACGTCAAGTAGCTCAAGCGTTC
ATTATTGAAGGATCAACTAGGAATACCCATCCGGATGGAGTTGAATTCATTCCGTTAGAGCCGGAAGTTAATACTAACTG
CGTTTTAGTTTGGAAGCGGGAGCGTAATCTATCACCGGTAGTAAATAAATTTATTAATTACTTCTACCATGCTTTCAAAG
CATAG

Upstream 100 bases:

>100_bases
GAGGTTGAGCTTTTTTAACGTATATATTACTCATGTAGCTAGGCTCCTTTTATTGTATGTTGTATATTAAGTATAGTATA
AAAAATTGGAGAATCGGGAA

Downstream 100 bases:

>100_bases
AAATGGATAAAATAAGCATTTTACGAATTACATAAAAAGTCTTAACATTATTTTTGTCGAAAGATGTTAAGGCTTTTTCT
AATCGGAGGTGCATAGTGGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MEIRTLRYFWTIAEEGTVSKAAEALHITQPTLSRQIKELEEELGTELFIRGRRQVRLTEAGMFLKSRAEEILELTQQTSL
EFENRKKQLFSGHITIGCIEADNSDTLALILEDFIRDYPQVTFTIYSGTSDDITERLDKGLVDVAVLIKPIATEKYHEIV
LPRTEKWGLLVSKASYIAQKDKIVPADLMGMPLLVSGRPEVQELISKWSKIDYQQLNVVGHYNLTFNVLPLVKRQVAQAF
IIEGSTRNTHPDGVEFIPLEPEVNTNCVLVWKRERNLSPVVNKFINYFYHAFKA

Sequences:

>Translated_294_residues
MEIRTLRYFWTIAEEGTVSKAAEALHITQPTLSRQIKELEEELGTELFIRGRRQVRLTEAGMFLKSRAEEILELTQQTSL
EFENRKKQLFSGHITIGCIEADNSDTLALILEDFIRDYPQVTFTIYSGTSDDITERLDKGLVDVAVLIKPIATEKYHEIV
LPRTEKWGLLVSKASYIAQKDKIVPADLMGMPLLVSGRPEVQELISKWSKIDYQQLNVVGHYNLTFNVLPLVKRQVAQAF
IIEGSTRNTHPDGVEFIPLEPEVNTNCVLVWKRERNLSPVVNKFINYFYHAFKA
>Mature_294_residues
MEIRTLRYFWTIAEEGTVSKAAEALHITQPTLSRQIKELEEELGTELFIRGRRQVRLTEAGMFLKSRAEEILELTQQTSL
EFENRKKQLFSGHITIGCIEADNSDTLALILEDFIRDYPQVTFTIYSGTSDDITERLDKGLVDVAVLIKPIATEKYHEIV
LPRTEKWGLLVSKASYIAQKDKIVPADLMGMPLLVSGRPEVQELISKWSKIDYQQLNVVGHYNLTFNVLPLVKRQVAQAF
IIEGSTRNTHPDGVEFIPLEPEVNTNCVLVWKRERNLSPVVNKFINYFYHAFKA

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=277, Percent_Identity=27.0758122743682, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788297, Length=155, Percent_Identity=33.5483870967742, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788887, Length=291, Percent_Identity=26.8041237113402, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790399, Length=195, Percent_Identity=30.2564102564103, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1790208, Length=150, Percent_Identity=36, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787879, Length=312, Percent_Identity=26.6025641025641, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787806, Length=152, Percent_Identity=28.9473684210526, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787601, Length=146, Percent_Identity=30.8219178082192, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788748, Length=284, Percent_Identity=24.6478873239437, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787530, Length=220, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI2367136, Length=120, Percent_Identity=35, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI145693105, Length=134, Percent_Identity=31.3432835820896, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790262, Length=195, Percent_Identity=29.2307692307692, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87081904, Length=146, Percent_Identity=26.7123287671233, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786202, Length=196, Percent_Identity=28.0612244897959, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788706, Length=167, Percent_Identity=29.3413173652695, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787589, Length=145, Percent_Identity=31.7241379310345, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33698; Mature: 33698

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRTLRYFWTIAEEGTVSKAAEALHITQPTLSRQIKELEEELGTELFIRGRRQVRLTEA
CCCEEEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCCEEEEHHH
GMFLKSRAEEILELTQQTSLEFENRKKQLFSGHITIGCIEADNSDTLALILEDFIRDYPQ
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCC
VTFTIYSGTSDDITERLDKGLVDVAVLIKPIATEKYHEIVLPRTEKWGLLVSKASYIAQK
EEEEEECCCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHEECCCCCCCCEEEHHHHHHHHC
DKIVPADLMGMPLLVSGRPEVQELISKWSKIDYQQLNVVGHYNLTFNVLPLVKRQVAQAF
CCCCCHHHCCCCEEECCCHHHHHHHHHHHHCCHHHEEEEEEECEEEEHHHHHHHHHHHEE
IIEGSTRNTHPDGVEFIPLEPEVNTNCVLVWKRERNLSPVVNKFINYFYHAFKA
EEECCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEIRTLRYFWTIAEEGTVSKAAEALHITQPTLSRQIKELEEELGTELFIRGRRQVRLTEA
CCCEEEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCCEEEEHHH
GMFLKSRAEEILELTQQTSLEFENRKKQLFSGHITIGCIEADNSDTLALILEDFIRDYPQ
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCC
VTFTIYSGTSDDITERLDKGLVDVAVLIKPIATEKYHEIVLPRTEKWGLLVSKASYIAQK
EEEEEECCCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHEECCCCCCCCEEEHHHHHHHHC
DKIVPADLMGMPLLVSGRPEVQELISKWSKIDYQQLNVVGHYNLTFNVLPLVKRQVAQAF
CCCCCHHHCCCCEEECCCHHHHHHHHHHHHCCHHHEEEEEEECEEEEHHHHHHHHHHHEE
IIEGSTRNTHPDGVEFIPLEPEVNTNCVLVWKRERNLSPVVNKFINYFYHAFKA
EEECCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]