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The map label for this gene is nfo

Identifier: 116492873

GI number: 116492873

Start: 1109126

End: 1110016

Strand: Reverse

Name: nfo

Synonym: PEPE_1111

Alternate gene names: 116492873

Gene position: 1110016-1109126 (Counterclockwise)

Preceding gene: 116492874

Following gene: 116492871

Centisome position: 60.58

GC content: 37.15

Gene sequence:

>891_bases
ATGATTATTGGTTCACACGTCAGTATGAGTGGAAGGAAAATGTTTTTAGGCTCCGTTGAAACGAGTATTGAAAATGGGGC
AAATGCTTTGATGATTTATACAGGAGCTCCCCAAAATACACGCCGCAAAGCAATTGAAGATTTACGTATTCCTGAAGGAC
GCGCCTTACTTACAGAACATGATTTTAAAGACGTCGTGGTTCATGCCCCATACATTGTTAATTTAGGAAATACTTTTAAA
CCCGAAAGTTTCAAATTCGCCGTGGAATTCTTAAAAGAAGAGGTTAAACGGGCTGATGCTTTAGGGGCTAGCCAGTTAGT
CTTACATCCAGGATCGCATGTAGGAGCAGGTCCCGACGCTGCGATTGCGTCAATTACTGAGGGATTAAATCAAATTATTA
CACCAGAGCAAAATGTGAAGATTGCGTTAGAAACCATGGCGGGAAAAGGAACTGAAGTTGCAACTAACTTTGAACAAATT
CAGCAAATTATTACTGGAGTAGAGCACAACGAAAAATTATCCGTATGTTTTGATACATGTCATACGAATGATGCGGGATA
TGATGTTAAAAACCATTTCGATGATGTAATGGATGAATTTGACCAAATTATTGGTTTGCAGAAAATCGGCGTAATTCATT
TAAATGATTCTAAAAATGAAATGGGCAGTCACAAAGACCGCCATGAAAATCTTGGGTTTGGAACCATTGGATTTAAGGCG
CTACATTATATTGCTAATTTTGATAAATTTCAAAACGTTCCTAAAATTTTAGAGACACCATGGGTAAAAGATCCAGATGC
TAAAAAGCATTCTCCATATCAACTAGAGCTTGCAATGCTTAAGAGTGGTAGATTTGATGATACATTGATTGAAAAAATCA
TCAATAATTAA

Upstream 100 bases:

>100_bases
CAGAGCGCTTTAACAAATTTGCCAAGCGTTTTGTAAGTTGGGAAAGAGGGACAGCTGCTCAACAAGTCATAGAAAAAATT
AACTATCGGAGGGATGAAAA

Downstream 100 bases:

>100_bases
AAAATGTCATAGGGCAATGTGATAACGAGATATTTATCTCGGTGCCTTATGACATTTTTTGGTTTTATACGAGACTTTCC
ATAATAATTGTGGCGGTCTC

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MIIGSHVSMSGRKMFLGSVETSIENGANALMIYTGAPQNTRRKAIEDLRIPEGRALLTEHDFKDVVVHAPYIVNLGNTFK
PESFKFAVEFLKEEVKRADALGASQLVLHPGSHVGAGPDAAIASITEGLNQIITPEQNVKIALETMAGKGTEVATNFEQI
QQIITGVEHNEKLSVCFDTCHTNDAGYDVKNHFDDVMDEFDQIIGLQKIGVIHLNDSKNEMGSHKDRHENLGFGTIGFKA
LHYIANFDKFQNVPKILETPWVKDPDAKKHSPYQLELAMLKSGRFDDTLIEKIINN

Sequences:

>Translated_296_residues
MIIGSHVSMSGRKMFLGSVETSIENGANALMIYTGAPQNTRRKAIEDLRIPEGRALLTEHDFKDVVVHAPYIVNLGNTFK
PESFKFAVEFLKEEVKRADALGASQLVLHPGSHVGAGPDAAIASITEGLNQIITPEQNVKIALETMAGKGTEVATNFEQI
QQIITGVEHNEKLSVCFDTCHTNDAGYDVKNHFDDVMDEFDQIIGLQKIGVIHLNDSKNEMGSHKDRHENLGFGTIGFKA
LHYIANFDKFQNVPKILETPWVKDPDAKKHSPYQLELAMLKSGRFDDTLIEKIINN
>Mature_296_residues
MIIGSHVSMSGRKMFLGSVETSIENGANALMIYTGAPQNTRRKAIEDLRIPEGRALLTEHDFKDVVVHAPYIVNLGNTFK
PESFKFAVEFLKEEVKRADALGASQLVLHPGSHVGAGPDAAIASITEGLNQIITPEQNVKIALETMAGKGTEVATNFEQI
QQIITGVEHNEKLSVCFDTCHTNDAGYDVKNHFDDVMDEFDQIIGLQKIGVIHLNDSKNEMGSHKDRHENLGFGTIGFKA
LHYIANFDKFQNVPKILETPWVKDPDAKKHSPYQLELAMLKSGRFDDTLIEKIINN

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=285, Percent_Identity=31.5789473684211, Blast_Score=141, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17531193, Length=276, Percent_Identity=31.1594202898551, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6322735, Length=267, Percent_Identity=31.0861423220974, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_PEDPA (Q03F56)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804608.1
- ProteinModelPortal:   Q03F56
- SMR:   Q03F56
- STRING:   Q03F56
- GeneID:   4417046
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1111
- NMPDR:   fig|278197.10.peg.975
- eggNOG:   COG0648
- HOGENOM:   HBG565018
- OMA:   QIALETM
- PhylomeDB:   Q03F56
- ProtClustDB:   PRK01060
- BioCyc:   PPEN278197:PEPE_1111-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 32810; Mature: 32810

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGSHVSMSGRKMFLGSVETSIENGANALMIYTGAPQNTRRKAIEDLRIPEGRALLTEH
CEECCCCCCCCCEEEEHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEECC
DFKDVVVHAPYIVNLGNTFKPESFKFAVEFLKEEVKRADALGASQLVLHPGSHVGAGPDA
CCCCEEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHH
AIASITEGLNQIITPEQNVKIALETMAGKGTEVATNFEQIQQIITGVEHNEKLSVCFDTC
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHCHHHHHHHHHCCCCCCEEEEEEEEC
HTNDAGYDVKNHFDDVMDEFDQIIGLQKIGVIHLNDSKNEMGSHKDRHENLGFGTIGFKA
CCCCCCCCHHHHHHHHHHHHHHHHCHHHEEEEEECCCHHHHCCCCHHHHCCCCCHHHHHH
LHYIANFDKFQNVPKILETPWVKDPDAKKHSPYQLELAMLKSGRFDDTLIEKIINN
HHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCEEEEEEEHHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MIIGSHVSMSGRKMFLGSVETSIENGANALMIYTGAPQNTRRKAIEDLRIPEGRALLTEH
CEECCCCCCCCCEEEEHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEECC
DFKDVVVHAPYIVNLGNTFKPESFKFAVEFLKEEVKRADALGASQLVLHPGSHVGAGPDA
CCCCEEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHH
AIASITEGLNQIITPEQNVKIALETMAGKGTEVATNFEQIQQIITGVEHNEKLSVCFDTC
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHCHHHHHHHHHCCCCCCEEEEEEEEC
HTNDAGYDVKNHFDDVMDEFDQIIGLQKIGVIHLNDSKNEMGSHKDRHENLGFGTIGFKA
CCCCCCCCHHHHHHHHHHHHHHHHCHHHEEEEEECCCHHHHCCCCHHHHCCCCCHHHHHH
LHYIANFDKFQNVPKILETPWVKDPDAKKHSPYQLELAMLKSGRFDDTLIEKIINN
HHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCEEEEEEEHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA