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The map label for this gene is cca

Identifier: 116492835

GI number: 116492835

Start: 1069116

End: 1070306

Strand: Reverse

Name: cca

Synonym: PEPE_1072

Alternate gene names: 116492835

Gene position: 1070306-1069116 (Counterclockwise)

Preceding gene: 116492837

Following gene: 116492834

Centisome position: 58.41

GC content: 37.53

Gene sequence:

>1191_bases
ATGCCCCAAGAATTTGAGGACGCCCGTGCAGTCCTACAAAAAATCGAAGATGCAGGTTTTGATGCTTTTTTTGTTGGGGG
AAGTGTTCGTGATACATTATTAAATAAGCCAATTCATGATGTTGATATTGCATCAAGCGCATACCCCGCCGAAATTAAAC
ATATTTTTAAGAAAACTGTTGATACAGGAATCGAACATGGTACGGTAATGGTGATTCATGATGGCGAAGGTTACGAGGTC
ACGACTTTTAGAACCGAAAGTGGCTACCAGGATTTTCGCCGTCCAGATCAGGTTACTTTTGTACGCTCTTTAAAAGAAGA
TTTAATGCGCCGTGATTTTACAATTAACGCATTTGCTTTAAAAGAAGATCGAACGGTAATTGACATTTTTGATGGTTTAA
GTGATCTAGAACATAAAATTATTCGTGCGGTAGGAGATCCACACGAACGTTTTCATGAAGATGCCTTAAGAATGATGCGA
GCAGTACGGTTTGCAAGCCAACTAGATTTCAAAATTGAAGCAAAGACGCTGAAGGCAATCGAAGAAAATAATATGCTTTT
AGGTAAAATCTCAGTCGAAAGAATCTTAGTTGAATTTGAAAAAATGATGTTGGGTAGCAAACCTAATCGTGGACTAGAAG
ACATGCTGACAACTAAATTGAATGAATATTGCCCGGGATTTAAGGACCGTTCTAAAGAACTAGACCAGCTTACAAAATAT
CCCTTGAAACTTCTAGAAAATCCTGAACAAGTTTGGACTATGATTGCTTGGAGTTTAAAATTAACTCCTCAAGAAGTAAT
GCCTTTCTTGAAACAATGGAAGACATCTAACGATTTGATCAAAAATGTCAGTGCGACTTTACAAGTATTAAGCATGGATA
AAAATGATCAAAACGAACGTTTAGCGCTGTTCAACGCAGGGGAAGCTAATGTAGTTAACGCCATTCGGGTGGCCCAAATT
CTGGGGATGAATCGTGAGGCTTGGCTAGATACTTACCAAAACCTTCAAATTAAAGATACACATGAAATGGCAATTACTGG
TAAAGAGCTAATTCAAAAGGGAATAATTAAGCCTGGACCTCAGATGGGACAAGTTTTAAACCGGTTAAAATTAATGGTTA
TTAATGATGAACTGAAAAACGAGTCCTTAGCGCTTTTGAATGCAGTAAATAAGTTACAGAAAGATGATTAA

Upstream 100 bases:

>100_bases
TCGGATTGATGCGCATACTAAGTTAAAGAATTTATTATTTAAGGTGTTACGAGAAAGTAATTCCTTTGAAACGGATGTGG
AAATGGTGCAGATAGAAAAA

Downstream 100 bases:

>100_bases
ATATGAAAATACTAAAAGGTAAGAATCTAACCAAAACTTACGGTGAAAAGAAGCTGTTTAACTCGATTAATTTTGTAATC
AATGAACACGATCGAGTTGG

Product: tRNA nucleotidyltransferase/poly(A) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MPQEFEDARAVLQKIEDAGFDAFFVGGSVRDTLLNKPIHDVDIASSAYPAEIKHIFKKTVDTGIEHGTVMVIHDGEGYEV
TTFRTESGYQDFRRPDQVTFVRSLKEDLMRRDFTINAFALKEDRTVIDIFDGLSDLEHKIIRAVGDPHERFHEDALRMMR
AVRFASQLDFKIEAKTLKAIEENNMLLGKISVERILVEFEKMMLGSKPNRGLEDMLTTKLNEYCPGFKDRSKELDQLTKY
PLKLLENPEQVWTMIAWSLKLTPQEVMPFLKQWKTSNDLIKNVSATLQVLSMDKNDQNERLALFNAGEANVVNAIRVAQI
LGMNREAWLDTYQNLQIKDTHEMAITGKELIQKGIIKPGPQMGQVLNRLKLMVINDELKNESLALLNAVNKLQKDD

Sequences:

>Translated_396_residues
MPQEFEDARAVLQKIEDAGFDAFFVGGSVRDTLLNKPIHDVDIASSAYPAEIKHIFKKTVDTGIEHGTVMVIHDGEGYEV
TTFRTESGYQDFRRPDQVTFVRSLKEDLMRRDFTINAFALKEDRTVIDIFDGLSDLEHKIIRAVGDPHERFHEDALRMMR
AVRFASQLDFKIEAKTLKAIEENNMLLGKISVERILVEFEKMMLGSKPNRGLEDMLTTKLNEYCPGFKDRSKELDQLTKY
PLKLLENPEQVWTMIAWSLKLTPQEVMPFLKQWKTSNDLIKNVSATLQVLSMDKNDQNERLALFNAGEANVVNAIRVAQI
LGMNREAWLDTYQNLQIKDTHEMAITGKELIQKGIIKPGPQMGQVLNRLKLMVINDELKNESLALLNAVNKLQKDD
>Mature_395_residues
PQEFEDARAVLQKIEDAGFDAFFVGGSVRDTLLNKPIHDVDIASSAYPAEIKHIFKKTVDTGIEHGTVMVIHDGEGYEVT
TFRTESGYQDFRRPDQVTFVRSLKEDLMRRDFTINAFALKEDRTVIDIFDGLSDLEHKIIRAVGDPHERFHEDALRMMRA
VRFASQLDFKIEAKTLKAIEENNMLLGKISVERILVEFEKMMLGSKPNRGLEDMLTTKLNEYCPGFKDRSKELDQLTKYP
LKLLENPEQVWTMIAWSLKLTPQEVMPFLKQWKTSNDLIKNVSATLQVLSMDKNDQNERLALFNAGEANVVNAIRVAQIL
GMNREAWLDTYQNLQIKDTHEMAITGKELIQKGIIKPGPQMGQVLNRLKLMVINDELKNESLALLNAVNKLQKDD

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=386, Percent_Identity=28.4974093264249, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI87081691, Length=212, Percent_Identity=34.9056603773585, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1789436, Length=263, Percent_Identity=29.6577946768061, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71995920, Length=213, Percent_Identity=36.150234741784, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321016, Length=213, Percent_Identity=29.5774647887324, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI281360112, Length=394, Percent_Identity=28.9340101522843, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21357337, Length=394, Percent_Identity=28.9340101522843, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_PEDPA (Q03F94)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804570.1
- ProteinModelPortal:   Q03F94
- SMR:   Q03F94
- STRING:   Q03F94
- GeneID:   4417076
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1072
- NMPDR:   fig|278197.10.peg.938
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   DHGNGYE
- PhylomeDB:   Q03F94
- BioCyc:   PPEN278197:PEPE_1072-MONOMER
- HAMAP:   MF_01263
- InterPro:   IPR023068
- InterPro:   IPR002646

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 45327; Mature: 45196

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: BINDING 27-27 BINDING 30-30 BINDING 111-111 BINDING 154-154 BINDING 157-157 BINDING 160-160 BINDING 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQEFEDARAVLQKIEDAGFDAFFVGGSVRDTLLNKPIHDVDIASSAYPAEIKHIFKKTV
CCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
DTGIEHGTVMVIHDGEGYEVTTFRTESGYQDFRRPDQVTFVRSLKEDLMRRDFTINAFAL
HCCCCCCEEEEEECCCCEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEE
KEDRTVIDIFDGLSDLEHKIIRAVGDPHERFHEDALRMMRAVRFASQLDFKIEAKTLKAI
ECCCEEEEHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHH
EENNMLLGKISVERILVEFEKMMLGSKPNRGLEDMLTTKLNEYCPGFKDRSKELDQLTKY
HHCCEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
PLKLLENPEQVWTMIAWSLKLTPQEVMPFLKQWKTSNDLIKNVSATLQVLSMDKNDQNER
HHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCE
LALFNAGEANVVNAIRVAQILGMNREAWLDTYQNLQIKDTHEMAITGKELIQKGIIKPGP
EEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCCCCH
QMGQVLNRLKLMVINDELKNESLALLNAVNKLQKDD
HHHHHHHHHEEEEECCHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PQEFEDARAVLQKIEDAGFDAFFVGGSVRDTLLNKPIHDVDIASSAYPAEIKHIFKKTV
CCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
DTGIEHGTVMVIHDGEGYEVTTFRTESGYQDFRRPDQVTFVRSLKEDLMRRDFTINAFAL
HCCCCCCEEEEEECCCCEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEE
KEDRTVIDIFDGLSDLEHKIIRAVGDPHERFHEDALRMMRAVRFASQLDFKIEAKTLKAI
ECCCEEEEHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHH
EENNMLLGKISVERILVEFEKMMLGSKPNRGLEDMLTTKLNEYCPGFKDRSKELDQLTKY
HHCCEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
PLKLLENPEQVWTMIAWSLKLTPQEVMPFLKQWKTSNDLIKNVSATLQVLSMDKNDQNER
HHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCE
LALFNAGEANVVNAIRVAQILGMNREAWLDTYQNLQIKDTHEMAITGKELIQKGIIKPGP
EEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCCCCH
QMGQVLNRLKLMVINDELKNESLALLNAVNKLQKDD
HHHHHHHHHEEEEECCHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA