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The map label for this gene is sigL [H]

Identifier: 116492259

GI number: 116492259

Start: 509598

End: 510914

Strand: Direct

Name: sigL [H]

Synonym: PEPE_0457

Alternate gene names: 116492259

Gene position: 509598-510914 (Clockwise)

Preceding gene: 116492257

Following gene: 116492260

Centisome position: 27.81

GC content: 37.28

Gene sequence:

>1317_bases
ATGGCAATAAAACAAGGATTTAGTCAAAATCAAACTCAACAACAAAAGCAAATCCAACGTTTAGCAATGACGCAGACCCT
ACAACAGTCGATTCAGATGTTGCAATATAACGTTGAAGAGCTTCAAGATTTTCTCAAACAAAAAGAAATGGATAATCCAC
TTATTACGGTTGAAATGGCTGCTGAAAAGCCAACCAGTAAGATTTCAGGTGATTCCGATCGACACGAGGCATTTATGAAC
CAAATTGCTAGCGATACGGAACAATCATTGTTCGACTACTTATTAGATCAGGTTCATTTAACCATGCGAGATACGCCATT
AAGACGCCTAGTATTGTTGCTTTTAGACTATGTTGACGATAATGGGTATTTAGGAATATCCAATGAAGCAGTGATTGAAA
AGACCGGTGCAGATGAAATTGCCGTGATCGATGCGGTAACTTTACTCCAACAGTTGGACCCGCCTGGCGTGGGGGCTCGC
TCGCTCCAAGAGTGTCTAATGCTTCAAACTGAAAATGATACATTTTCTCCAGAACAAGCTTATTTGATTTTGGAAGAATC
TTTTGAAGATTTAGTCAATCGTCAATGGGATAAAATCGCTAAAAAATATGATATTCAATTGAGTGATGTCCAAGCGGTTT
TTGATTATGTCAGAACATTAACCCCTAACCCCGGAGCAGGATTTGGAAAACAAGAGCATAGTTATATTATTCCAGATTTA
ATCTTTAGCATTGAAAATGGTGAACCTCAATTACTACAGTCTAAGGCTTCACGTCCTAAAGTCAGCTTCTCACAACATTA
TTTTGAACAACTTCAAAACGTTGATGAAGAGGTCAAAGCTTATGTCACGGAGAAGAAGAAAGAATACGACTGGATTTCGA
AGAGTTTAAGTCAACGGGAAGATACCATTCTCAGAGTTGGAAAGATTATCTTTGAAAAGCAAAGTGAATTCTTTATGAAT
CAAAGTAAAGAGCTAAAACCGTTGCTTCTAAGAGATGTCGCGAATAAATTACATTTACATGAATCAACAATATCTCGTGC
TGTTAACGGTAAATATATTGCAACCCCATTTGGTACGTTTGAACTGAAATCGTTCTTTAGTAATGCTGTTAATCGTGAAA
GTGATGTTTCAGCAGATGCAGCAAAAGAAAAGATTAAAAAAATTATCGATGAAGAGGATAAAAGAAAACCTATTTCTGAT
TCGAAAATTGAGAAAAATTTAAAAGAAAGTGGGATTGCGATTTCGCGGAGAACCGTCGCCAAATACCGCGAAGCCATTGG
GATTCCATCTTCTTCAAAACGCAAGCGATTTGATTAA

Upstream 100 bases:

>100_bases
GAACCGGAATCTCACGTGCTATCCATTACACTAACGGCGCCTAACAATTTGATTTTACAGTAGGACAGAAGAAAAAACAA
GATAATTGCGGAGAATTACT

Downstream 100 bases:

>100_bases
TTTAAAAAGCCCCAAATAAGTTGAGTTTAAATTTGCAAAAAAGATGGGGGCTTGTTATAATTTAATTCGTAAGTGAGGTA
TACAACAAAAGTTAATTCTT

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MAIKQGFSQNQTQQQKQIQRLAMTQTLQQSIQMLQYNVEELQDFLKQKEMDNPLITVEMAAEKPTSKISGDSDRHEAFMN
QIASDTEQSLFDYLLDQVHLTMRDTPLRRLVLLLLDYVDDNGYLGISNEAVIEKTGADEIAVIDAVTLLQQLDPPGVGAR
SLQECLMLQTENDTFSPEQAYLILEESFEDLVNRQWDKIAKKYDIQLSDVQAVFDYVRTLTPNPGAGFGKQEHSYIIPDL
IFSIENGEPQLLQSKASRPKVSFSQHYFEQLQNVDEEVKAYVTEKKKEYDWISKSLSQREDTILRVGKIIFEKQSEFFMN
QSKELKPLLLRDVANKLHLHESTISRAVNGKYIATPFGTFELKSFFSNAVNRESDVSADAAKEKIKKIIDEEDKRKPISD
SKIEKNLKESGIAISRRTVAKYREAIGIPSSSKRKRFD

Sequences:

>Translated_438_residues
MAIKQGFSQNQTQQQKQIQRLAMTQTLQQSIQMLQYNVEELQDFLKQKEMDNPLITVEMAAEKPTSKISGDSDRHEAFMN
QIASDTEQSLFDYLLDQVHLTMRDTPLRRLVLLLLDYVDDNGYLGISNEAVIEKTGADEIAVIDAVTLLQQLDPPGVGAR
SLQECLMLQTENDTFSPEQAYLILEESFEDLVNRQWDKIAKKYDIQLSDVQAVFDYVRTLTPNPGAGFGKQEHSYIIPDL
IFSIENGEPQLLQSKASRPKVSFSQHYFEQLQNVDEEVKAYVTEKKKEYDWISKSLSQREDTILRVGKIIFEKQSEFFMN
QSKELKPLLLRDVANKLHLHESTISRAVNGKYIATPFGTFELKSFFSNAVNRESDVSADAAKEKIKKIIDEEDKRKPISD
SKIEKNLKESGIAISRRTVAKYREAIGIPSSSKRKRFD
>Mature_437_residues
AIKQGFSQNQTQQQKQIQRLAMTQTLQQSIQMLQYNVEELQDFLKQKEMDNPLITVEMAAEKPTSKISGDSDRHEAFMNQ
IASDTEQSLFDYLLDQVHLTMRDTPLRRLVLLLLDYVDDNGYLGISNEAVIEKTGADEIAVIDAVTLLQQLDPPGVGARS
LQECLMLQTENDTFSPEQAYLILEESFEDLVNRQWDKIAKKYDIQLSDVQAVFDYVRTLTPNPGAGFGKQEHSYIIPDLI
FSIENGEPQLLQSKASRPKVSFSQHYFEQLQNVDEEVKAYVTEKKKEYDWISKSLSQREDTILRVGKIIFEKQSEFFMNQ
SKELKPLLLRDVANKLHLHESTISRAVNGKYIATPFGTFELKSFFSNAVNRESDVSADAAKEKIKKIIDEEDKRKPISDS
KIEKNLKESGIAISRRTVAKYREAIGIPSSSKRKRFD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the levanase operon. The open complex (sigma-54 and core RNA poly

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=484, Percent_Identity=33.6776859504132, Blast_Score=244, Evalue=7e-66,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 50150; Mature: 50019

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKQGFSQNQTQQQKQIQRLAMTQTLQQSIQMLQYNVEELQDFLKQKEMDNPLITVEMA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
AEKPTSKISGDSDRHEAFMNQIASDTEQSLFDYLLDQVHLTMRDTPLRRLVLLLLDYVDD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
NGYLGISNEAVIEKTGADEIAVIDAVTLLQQLDPPGVGARSLQECLMLQTENDTFSPEQA
CCEEECCCCHHEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEECCCCCCCCHHH
YLILEESFEDLVNRQWDKIAKKYDIQLSDVQAVFDYVRTLTPNPGAGFGKQEHSYIIPDL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHH
IFSIENGEPQLLQSKASRPKVSFSQHYFEQLQNVDEEVKAYVTEKKKEYDWISKSLSQRE
HHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTILRVGKIIFEKQSEFFMNQSKELKPLLLRDVANKLHLHESTISRAVNGKYIATPFGTF
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHH
ELKSFFSNAVNRESDVSADAAKEKIKKIIDEEDKRKPISDSKIEKNLKESGIAISRRTVA
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHCCCHHHHHHHH
KYREAIGIPSSSKRKRFD
HHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AIKQGFSQNQTQQQKQIQRLAMTQTLQQSIQMLQYNVEELQDFLKQKEMDNPLITVEMA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC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HHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1924373; 8969506; 9384377 [H]