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The map label for this gene is prfB [H]

Identifier: 116492233

GI number: 116492233

Start: 480958

End: 481989

Strand: Direct

Name: prfB [H]

Synonym: PEPE_0430

Alternate gene names: 116492233

Gene position: 480958-481989 (Clockwise)

Preceding gene: 116492232

Following gene: 116492234

Centisome position: 26.25

GC content: 39.83

Gene sequence:

>1032_bases
TTGAATGAAAGTATCAGTGTCAATGAGGCCAAAATGGCAGAGCCTAACTTTTGGGATAATCAAGAACAGGCTCAAATTGT
CATCAATGAAAATAATGAGTTAAAACAAAAATACGATAATTTTAAAAATTTAACCGACCATGTTGAAAACTTAGAGGTAA
CTGCTGAATTATTGGAAATGGAAGATGATCCTGACGTTCAAAGTGAGTTTGAGAGTGAATTAGCCGCCACGGCTAAATTA
TTGCAGGATTACCGTTTAGGATTATTGCTTAATGGTAAATATGATGCCAATAACGCAATTATAGAAATTCACCCCGGAGC
GGGAGGAACAGAATCTCAAGATTGGGGTTCCATGTTGCTAAGAATGTACACGCGTTGGGCTGAGCAGCATCAGTTTAAGG
TTCAAATTGAAGATTACCAAGCAGGTGAAGTGGCAGGAATTAGTAGTGTAACCATGCGAATTGTCGGTCACAACGCATAT
GGTTATTTACGCTCTGAAAAGGGAGTTCATCGGCTAGTAAGACTATCACCATTTGATTCTGCTGGACGACGGCATACTTC
TTTTGCATCAGTCGACGTTATGCCGGAATTGGATGATTCGGTTGAAGTTAACATTAACCAAGATGATTTACGGGTGGATG
TATATCGTTCTAGTGGTGCTGGTGGACAGCATATTAACAAGACTTCTTCAGCGGTGCGAATTACGCATTTGCCAACTGGA
ATTGTAGTTGCGAGTCAAGCAGAACGTTCTCAATTACAAAACCGGGCTACCGCTATGAGTATGTTGAAGGCTAAGCTATT
TGAGCTTGAAGAACAAAAGAAAGCAGAAGAAAAGGCTAAAATAGAAGGCATTCAACTTGAAATAGGATGGGGATCGCAGA
TTCGTTCTTACGTTTTCCATCCCTATTCTATGGTTAAAGATCATCGAAGCAACTTTGAGACGGCTAACGTTCAAGGTGTG
ATGGACGGGGATTTCGATGATTTTATCAATGCTTATTTGCAATGGAAATTGAAAGAAGATAATCCAAAATAG

Upstream 100 bases:

>100_bases
AAGGAGCTAACTTATATGGAGCTAGTTGAAGCAAAACATCAAATTGAAGATATGAAAAAAGAAGTCGCTGACTTCGGGAG
GTCTCTTTGACTTAGATGCT

Downstream 100 bases:

>100_bases
GGGGATTCGGATGTCAAAGACGATATTAGTTGTAGATGATGAGCCAGCAATTGTGACTTTAATTACTTATAATTTGGAAC
AAGCTAATTATCAAGTTGTG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MNESISVNEAKMAEPNFWDNQEQAQIVINENNELKQKYDNFKNLTDHVENLEVTAELLEMEDDPDVQSEFESELAATAKL
LQDYRLGLLLNGKYDANNAIIEIHPGAGGTESQDWGSMLLRMYTRWAEQHQFKVQIEDYQAGEVAGISSVTMRIVGHNAY
GYLRSEKGVHRLVRLSPFDSAGRRHTSFASVDVMPELDDSVEVNINQDDLRVDVYRSSGAGGQHINKTSSAVRITHLPTG
IVVASQAERSQLQNRATAMSMLKAKLFELEEQKKAEEKAKIEGIQLEIGWGSQIRSYVFHPYSMVKDHRSNFETANVQGV
MDGDFDDFINAYLQWKLKEDNPK

Sequences:

>Translated_343_residues
MNESISVNEAKMAEPNFWDNQEQAQIVINENNELKQKYDNFKNLTDHVENLEVTAELLEMEDDPDVQSEFESELAATAKL
LQDYRLGLLLNGKYDANNAIIEIHPGAGGTESQDWGSMLLRMYTRWAEQHQFKVQIEDYQAGEVAGISSVTMRIVGHNAY
GYLRSEKGVHRLVRLSPFDSAGRRHTSFASVDVMPELDDSVEVNINQDDLRVDVYRSSGAGGQHINKTSSAVRITHLPTG
IVVASQAERSQLQNRATAMSMLKAKLFELEEQKKAEEKAKIEGIQLEIGWGSQIRSYVFHPYSMVKDHRSNFETANVQGV
MDGDFDDFINAYLQWKLKEDNPK
>Mature_343_residues
MNESISVNEAKMAEPNFWDNQEQAQIVINENNELKQKYDNFKNLTDHVENLEVTAELLEMEDDPDVQSEFESELAATAKL
LQDYRLGLLLNGKYDANNAIIEIHPGAGGTESQDWGSMLLRMYTRWAEQHQFKVQIEDYQAGEVAGISSVTMRIVGHNAY
GYLRSEKGVHRLVRLSPFDSAGRRHTSFASVDVMPELDDSVEVNINQDDLRVDVYRSSGAGGQHINKTSSAVRITHLPTG
IVVASQAERSQLQNRATAMSMLKAKLFELEEQKKAEEKAKIEGIQLEIGWGSQIRSYVFHPYSMVKDHRSNFETANVQGV
MDGDFDDFINAYLQWKLKEDNPK

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=302, Percent_Identity=33.7748344370861, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI34577120, Length=318, Percent_Identity=31.7610062893082, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI166795305, Length=203, Percent_Identity=34.4827586206897, Blast_Score=115, Evalue=7e-26,
Organism=Escherichia coli, GI2367172, Length=328, Percent_Identity=50, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI1787462, Length=241, Percent_Identity=43.5684647302905, Blast_Score=190, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=223, Percent_Identity=34.9775784753363, Blast_Score=104, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6321295, Length=324, Percent_Identity=35.4938271604938, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI19921226, Length=230, Percent_Identity=37.3913043478261, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 38848; Mature: 38848

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNESISVNEAKMAEPNFWDNQEQAQIVINENNELKQKYDNFKNLTDHVENLEVTAELLEM
CCCCCCCCCHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EDDPDVQSEFESELAATAKLLQDYRLGLLLNGKYDANNAIIEIHPGAGGTESQDWGSMLL
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHH
RMYTRWAEQHQFKVQIEDYQAGEVAGISSVTMRIVGHNAYGYLRSEKGVHRLVRLSPFDS
HHHHHHHHCCEEEEEEECCCCCCCCCHHHEEEEEEECCCCEEEECCCCHHHHEEECCCCC
AGRRHTSFASVDVMPELDDSVEVNINQDDLRVDVYRSSGAGGQHINKTSSAVRITHLPTG
CCCCCCCEEEEECCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCC
IVVASQAERSQLQNRATAMSMLKAKLFELEEQKKAEEKAKIEGIQLEIGWGSQIRSYVFH
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHCC
PYSMVKDHRSNFETANVQGVMDGDFDDFINAYLQWKLKEDNPK
CHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHEEEEECCCCCC
>Mature Secondary Structure
MNESISVNEAKMAEPNFWDNQEQAQIVINENNELKQKYDNFKNLTDHVENLEVTAELLEM
CCCCCCCCCHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EDDPDVQSEFESELAATAKLLQDYRLGLLLNGKYDANNAIIEIHPGAGGTESQDWGSMLL
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHH
RMYTRWAEQHQFKVQIEDYQAGEVAGISSVTMRIVGHNAYGYLRSEKGVHRLVRLSPFDS
HHHHHHHHCCEEEEEEECCCCCCCCCHHHEEEEEEECCCCEEEECCCCHHHHEEECCCCC
AGRRHTSFASVDVMPELDDSVEVNINQDDLRVDVYRSSGAGGQHINKTSSAVRITHLPTG
CCCCCCCEEEEECCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCC
IVVASQAERSQLQNRATAMSMLKAKLFELEEQKKAEEKAKIEGIQLEIGWGSQIRSYVFH
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHCC
PYSMVKDHRSNFETANVQGVMDGDFDDFINAYLQWKLKEDNPK
CHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]