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The map label for this gene is groEL

Identifier: 116492224

GI number: 116492224

Start: 469788

End: 471407

Strand: Direct

Name: groEL

Synonym: PEPE_0421

Alternate gene names: 116492224

Gene position: 469788-471407 (Clockwise)

Preceding gene: 116492223

Following gene: 116492225

Centisome position: 25.64

GC content: 41.42

Gene sequence:

>1620_bases
ATGGCTAAAGAAATTAAATTTTCAGAAGATGCACGTACAAAAATGCTTAAGGGTGTAGATAAGTTAGCTGACACCGTTAA
GTCAACAATTGGACCTAAAGGCCGTAATGTGGTCTTGGAACAATCATACGGTTCACCTACAATTACTAATGATGGTGTGA
CAATTGCTAAGGCAATCGAACTTGAAGATCATTTTGAAAATATGGGTGCTAAGTTAGTTTCAGAAGTTGCTTCAAAGACC
AACGACATTGCTGGTGATGGTACAACTACCGCTACGGTTCTTACTCAAGCAATCGTCAATGAAGGAATGAAGAACGTTAC
TGCTGGTGCTAACCCTGTTGGTATCCGTCGCGGTATTGAAAAAGCAACAAGTAAAGCAGTTGAAGCTCTCCACAAGATGT
CACATGAAGTAAAAACTAAAGATGATATTGCTCAAATCGCTTCAATTTCTTCTGCTAACCCAGAAGTTGGCAAGTTGATT
GCTAACGCTATGGAAAAAGTTGGTAACGATGGTGTTATCACCATTGAAGAATCTCGTGGAGTTGACACAACATTGGATGT
TGTGGAAGGTATGCAATTTGACCGTGGATACATGTCACAATACATGGTAACTGACAACGACAAGATGGAAGCTAACCTTG
ATAATCCATACATCTTAATCACTGATAAGAAGATCGGTAATATCCAAGATATTCTTCCAGTTCTTCAATCAGTAGTTGAA
CAAAGCCGTTCATTATTGATTATTGCGGATGACATTACAGGTGAAGCTCTACCAACACTTGTTTTGAATAAGATGCGTGG
AACCTTCAACGTAGTTGCAGTTAAGGCTCCTGGCTTTGGTGATCGTCGTAAGGAACAATTGGAAGACATTGCTATCCTAA
CTGGTGGTACTGTAATTACAGATGACCTTGGTTTGAACTTGAAGGACGTTACTATCGAACAACTTGGTCAATCTAACAAG
GTTACAGTTACTAAAGATGATACAACCATCGTTGAAGGTGCTGGAAGCCAAGAACAAATCGCTGAACGTGTTGGAATTAT
CAAACAACAAATCAGTGAAACAACTTCTGACTTCGACAAGGAAAAACTTCAAGAACGTCTTGCTAAGTTATCTGGTGGTG
TTGCAGTAATCCGTGTTGGTGCTGCTACTGAAACAGAACTTAAAGAAAAGAAATACCGTATCGAAGATGCTTTAAACGCT
ACTAGAGCTGCCGTTGAAGAAGGTTTTGTTCCTGGTGGTGGTACAGCACTTGCTAACGTTATTTCAGACCTTGAAGACGT
CGAAGCTGAAGGCGATGAAGCTACAGGTGTTAACATTGTACGTCGTGCGCTTGAAGAACCAGTTCGTCAAATTGCTGAAA
ATGCCGGCGAAGAAGGTTCAGTAATCGTTACAAAACTTAAGGGCCAAAAGCCAGGTGTTGGATACAACGCCGCTAACGAC
GAATGGGTCGACATGGTTGAAGCCGGAATCGTTGATCCTACAAAGGTTACACGTTCAGCACTTCAAAATGCTGCTAGTGT
ATCAGCATTATTGTTAACTACTGAAGCAGTAGTTGCTGACTTACCAGAAGACAACCCAGCTCCTGCAGCACCAGCTCCAG
GTATGGGCGGTATGATGTAA

Upstream 100 bases:

>100_bases
TACTGAAGTTTCATACGAAGATAAAAAATACTTAGTTGTCCATGAAAAGGACTTAGTTGCAGTAGTTGAATAATTAATTT
TTAATTTAGGAGTGTTGATA

Downstream 100 bases:

>100_bases
TTTAATTCTTAGAACTAACGATTTAAATTAGGAAGATGCGAAGGTTTGCCGAAAATTCAGCTCTCCAACGCTAAACTAGA
CTAATTTAAATTAAAAGAAT

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MAKEIKFSEDARTKMLKGVDKLADTVKSTIGPKGRNVVLEQSYGSPTITNDGVTIAKAIELEDHFENMGAKLVSEVASKT
NDIAGDGTTTATVLTQAIVNEGMKNVTAGANPVGIRRGIEKATSKAVEALHKMSHEVKTKDDIAQIASISSANPEVGKLI
ANAMEKVGNDGVITIEESRGVDTTLDVVEGMQFDRGYMSQYMVTDNDKMEANLDNPYILITDKKIGNIQDILPVLQSVVE
QSRSLLIIADDITGEALPTLVLNKMRGTFNVVAVKAPGFGDRRKEQLEDIAILTGGTVITDDLGLNLKDVTIEQLGQSNK
VTVTKDDTTIVEGAGSQEQIAERVGIIKQQISETTSDFDKEKLQERLAKLSGGVAVIRVGAATETELKEKKYRIEDALNA
TRAAVEEGFVPGGGTALANVISDLEDVEAEGDEATGVNIVRRALEEPVRQIAENAGEEGSVIVTKLKGQKPGVGYNAAND
EWVDMVEAGIVDPTKVTRSALQNAASVSALLLTTEAVVADLPEDNPAPAAPAPGMGGMM

Sequences:

>Translated_539_residues
MAKEIKFSEDARTKMLKGVDKLADTVKSTIGPKGRNVVLEQSYGSPTITNDGVTIAKAIELEDHFENMGAKLVSEVASKT
NDIAGDGTTTATVLTQAIVNEGMKNVTAGANPVGIRRGIEKATSKAVEALHKMSHEVKTKDDIAQIASISSANPEVGKLI
ANAMEKVGNDGVITIEESRGVDTTLDVVEGMQFDRGYMSQYMVTDNDKMEANLDNPYILITDKKIGNIQDILPVLQSVVE
QSRSLLIIADDITGEALPTLVLNKMRGTFNVVAVKAPGFGDRRKEQLEDIAILTGGTVITDDLGLNLKDVTIEQLGQSNK
VTVTKDDTTIVEGAGSQEQIAERVGIIKQQISETTSDFDKEKLQERLAKLSGGVAVIRVGAATETELKEKKYRIEDALNA
TRAAVEEGFVPGGGTALANVISDLEDVEAEGDEATGVNIVRRALEEPVRQIAENAGEEGSVIVTKLKGQKPGVGYNAAND
EWVDMVEAGIVDPTKVTRSALQNAASVSALLLTTEAVVADLPEDNPAPAAPAPGMGGMM
>Mature_538_residues
AKEIKFSEDARTKMLKGVDKLADTVKSTIGPKGRNVVLEQSYGSPTITNDGVTIAKAIELEDHFENMGAKLVSEVASKTN
DIAGDGTTTATVLTQAIVNEGMKNVTAGANPVGIRRGIEKATSKAVEALHKMSHEVKTKDDIAQIASISSANPEVGKLIA
NAMEKVGNDGVITIEESRGVDTTLDVVEGMQFDRGYMSQYMVTDNDKMEANLDNPYILITDKKIGNIQDILPVLQSVVEQ
SRSLLIIADDITGEALPTLVLNKMRGTFNVVAVKAPGFGDRRKEQLEDIAILTGGTVITDDLGLNLKDVTIEQLGQSNKV
TVTKDDTTIVEGAGSQEQIAERVGIIKQQISETTSDFDKEKLQERLAKLSGGVAVIRVGAATETELKEKKYRIEDALNAT
RAAVEEGFVPGGGTALANVISDLEDVEAEGDEATGVNIVRRALEEPVRQIAENAGEEGSVIVTKLKGQKPGVGYNAANDE
WVDMVEAGIVDPTKVTRSALQNAASVSALLLTTEAVVADLPEDNPAPAAPAPGMGGMM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=530, Percent_Identity=50.377358490566, Blast_Score=514, Evalue=1e-146,
Organism=Homo sapiens, GI31542947, Length=530, Percent_Identity=50.377358490566, Blast_Score=514, Evalue=1e-146,
Organism=Homo sapiens, GI24307939, Length=176, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI38455427, Length=139, Percent_Identity=32.3741007194245, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI302058290, Length=517, Percent_Identity=21.0831721470019, Blast_Score=67, Evalue=6e-11,
Organism=Homo sapiens, GI5453603, Length=147, Percent_Identity=36.0544217687075, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI1790586, Length=528, Percent_Identity=58.5227272727273, Blast_Score=615, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI17555558, Length=528, Percent_Identity=49.6212121212121, Blast_Score=507, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI193210679, Length=245, Percent_Identity=48.5714285714286, Blast_Score=209, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI25144674, Length=170, Percent_Identity=28.2352941176471, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25147750, Length=145, Percent_Identity=35.8620689655172, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323288, Length=524, Percent_Identity=54.5801526717557, Blast_Score=549, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6322524, Length=121, Percent_Identity=30.5785123966942, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320058, Length=165, Percent_Identity=29.0909090909091, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322350, Length=527, Percent_Identity=23.3396584440228, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24641193, Length=527, Percent_Identity=51.0436432637571, Blast_Score=525, Evalue=1e-149,
Organism=Drosophila melanogaster, GI24641191, Length=527, Percent_Identity=51.0436432637571, Blast_Score=525, Evalue=1e-149,
Organism=Drosophila melanogaster, GI45550936, Length=524, Percent_Identity=48.4732824427481, Blast_Score=497, Evalue=1e-141,
Organism=Drosophila melanogaster, GI45550132, Length=524, Percent_Identity=48.4732824427481, Blast_Score=497, Evalue=1e-141,
Organism=Drosophila melanogaster, GI45550935, Length=524, Percent_Identity=48.4732824427481, Blast_Score=497, Evalue=1e-141,
Organism=Drosophila melanogaster, GI17864606, Length=541, Percent_Identity=42.5138632162662, Blast_Score=429, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24584129, Length=533, Percent_Identity=35.4596622889306, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI19921262, Length=533, Percent_Identity=35.4596622889306, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24645179, Length=555, Percent_Identity=22.7027027027027, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24583944, Length=179, Percent_Identity=27.3743016759777, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17647245, Length=184, Percent_Identity=27.1739130434783, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24652903, Length=173, Percent_Identity=27.7456647398844, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI28571140, Length=148, Percent_Identity=33.7837837837838, Blast_Score=66, Evalue=6e-11,
Organism=Drosophila melanogaster, GI18858175, Length=148, Percent_Identity=33.7837837837838, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_PEDPA (Q03H05)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_803959.1
- ProteinModelPortal:   Q03H05
- SMR:   Q03H05
- STRING:   Q03H05
- GeneID:   4418387
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0421
- NMPDR:   fig|278197.10.peg.391
- eggNOG:   COG0459
- HOGENOM:   HBG625289
- OMA:   DKPAAGM
- PhylomeDB:   Q03H05
- ProtClustDB:   PRK00013
- BioCyc:   PPEN278197:PEPE_0421-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 57223; Mature: 57092

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEIKFSEDARTKMLKGVDKLADTVKSTIGPKGRNVVLEQSYGSPTITNDGVTIAKAIE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEEHHH
LEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVNEGMKNVTAGANPVGIRRGIE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHH
KATSKAVEALHKMSHEVKTKDDIAQIASISSANPEVGKLIANAMEKVGNDGVITIEESRG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCC
VDTTLDVVEGMQFDRGYMSQYMVTDNDKMEANLDNPYILITDKKIGNIQDILPVLQSVVE
CCHHHHHHHCCCCCCHHHHHEEECCCCCCEECCCCCEEEEECCCCCCHHHHHHHHHHHHH
QSRSLLIIADDITGEALPTLVLNKMRGTFNVVAVKAPGFGDRRKEQLEDIAILTGGTVIT
CCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHEEEEECCEEEE
DDLGLNLKDVTIEQLGQSNKVTVTKDDTTIVEGAGSQEQIAERVGIIKQQISETTSDFDK
CCCCCCCHHHHHHHCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKLQERLAKLSGGVAVIRVGAATETELKEKKYRIEDALNATRAAVEEGFVPGGGTALANV
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ISDLEDVEAEGDEATGVNIVRRALEEPVRQIAENAGEEGSVIVTKLKGQKPGVGYNAAND
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCH
EWVDMVEAGIVDPTKVTRSALQNAASVSALLLTTEAVVADLPEDNPAPAAPAPGMGGMM
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKEIKFSEDARTKMLKGVDKLADTVKSTIGPKGRNVVLEQSYGSPTITNDGVTIAKAIE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEEHHH
LEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVNEGMKNVTAGANPVGIRRGIE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHH
KATSKAVEALHKMSHEVKTKDDIAQIASISSANPEVGKLIANAMEKVGNDGVITIEESRG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCC
VDTTLDVVEGMQFDRGYMSQYMVTDNDKMEANLDNPYILITDKKIGNIQDILPVLQSVVE
CCHHHHHHHCCCCCCHHHHHEEECCCCCCEECCCCCEEEEECCCCCCHHHHHHHHHHHHH
QSRSLLIIADDITGEALPTLVLNKMRGTFNVVAVKAPGFGDRRKEQLEDIAILTGGTVIT
CCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHEEEEECCEEEE
DDLGLNLKDVTIEQLGQSNKVTVTKDDTTIVEGAGSQEQIAERVGIIKQQISETTSDFDK
CCCCCCCHHHHHHHCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKLQERLAKLSGGVAVIRVGAATETELKEKKYRIEDALNATRAAVEEGFVPGGGTALANV
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ISDLEDVEAEGDEATGVNIVRRALEEPVRQIAENAGEEGSVIVTKLKGQKPGVGYNAAND
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCH
EWVDMVEAGIVDPTKVTRSALQNAASVSALLLTTEAVVADLPEDNPAPAAPAPGMGGMM
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA