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The map label for this gene is lmrB [H]

Identifier: 116492219

GI number: 116492219

Start: 463663

End: 465063

Strand: Direct

Name: lmrB [H]

Synonym: PEPE_0416

Alternate gene names: 116492219

Gene position: 463663-465063 (Clockwise)

Preceding gene: 116492218

Following gene: 116492220

Centisome position: 25.3

GC content: 38.54

Gene sequence:

>1401_bases
ATGCAAGAATCATCAACAAAAAAATCCAATCCGATGGTAGTTGTGGCGATTGTGGCACTGATGTCTTTTATGGGAGTTTT
AACGGAAACTTCAATGAACGTTACTTTCCCAGCTTTGATGCGAGAATTTAAGGTGTCATTAAGTACAGTGCAGTGGGTAA
CGGCAGGCTATTTGTTAATGGCTGCGTTAGTCATGTTAACTTCAGCTTACATGAAAAGAAGATTTACCAATCGACAGTTA
TTTGTGGCAGCAGCCATCTTGTTTGGATTGGGAGATGTTATCTGTGGCGCAGCGCTGAATTTTTGGGTGCTCTTGGTAGG
CCGTTTAATTCAAGCCGGATGTGTGGGGTTATGTACTCCTTTAATGGTTAATATAATTTTAGAGGTTGTACCCCAACACA
AGCTAGGAACTTATATTGGGTTAGCCAATTTAATTATTTTAATTGCGCCTGCCATGGGTCCGACCTTTGGAGGGGCCGTG
GTCGCTTTTGCAAGTTGGCGAATGATTTTCTGGGGAACATTACCGATTGCATTGATTCTAATGGTTTTAGGGTCTAAAAA
AATTAAACAATATACCCCAACTCAAAAACAGTATGCTTTTGATTGGACACGTTTTATTCTTTTAGGGTTGGCACTAGTAG
CCCTAATCGTTGGACTCAATAATTTGGGGTCAGGCGCTTATATTTCATTTATCGTTTTACTATTATTTAGTATTGGTTTA
ACAACGCTGTTTGTAAAAAAATCTAAAAATGCTACTAAAGCTTTATTTTCACTAGACGTATTCAAAAATAAGGCCTTTCT
ATTTAGTTTTCTACCATATATAATGTTGCAATTCGCTAACGTGGGGATTAACTTTTTACTTCCCAACTACGTTCAAGATG
TATTCCAAGCGACATCCTTAATTGGTGGTTTGATTTTGCTTCCAGGGAGTCTATTAAATGGATTTGGACAACCAGTTTAT
GGTTGGATGTTAGATAACTTTGGCGGAAAATTACCATTATATATGGGTGATATTTTATTTACCTTAGCGCTAGCATCCTT
AGCCCTTTGGGGACCACATATGGGAGTATTGGGAATTACCATAGCATACTTAATTTTTGCCATTGGCCGTTCTATGGCGT
TTGGTAATTCGGTTGCCTATGGTTTAAAACATATCAACCAAGAACTTCAAAATGACGCTAATGCGCTATATAACACCGGT
CAACAGGTGGCTGGTGCGATTGGAACGACGGTTTTAGCGTTAATGATGGGTTCAGTTCATCATCCAGGTTATACCAGAGC
CCAAAATATCAGTGCGGGCAGTGGATGGGCGTTTATTCTACTTGTTATTTTAGGATTAATTATCTTGGGCTTCTTTCATC
GATTGGTAAATTTAGATAAGCAAATTAAACAGACGAATTAA

Upstream 100 bases:

>100_bases
GTTACGTCTTTTTTGATGAACCAATTTGACAAATCTTTCAATTATTTCCATTATAGAAGTTATGTACTTTTAAAAAGTAA
CCTTGAGAGGAAGAGTTTTA

Downstream 100 bases:

>100_bases
TCGTGAAGTTGTATAATTAAAATTGTATTATAAAATAAGGAGAAAACTATGGCTAAAATTACGCGTCTTTATGATTTATT
TCAACCCGAACACTACGATA

Product: major facilitator superfamily permease

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MQESSTKKSNPMVVVAIVALMSFMGVLTETSMNVTFPALMREFKVSLSTVQWVTAGYLLMAALVMLTSAYMKRRFTNRQL
FVAAAILFGLGDVICGAALNFWVLLVGRLIQAGCVGLCTPLMVNIILEVVPQHKLGTYIGLANLIILIAPAMGPTFGGAV
VAFASWRMIFWGTLPIALILMVLGSKKIKQYTPTQKQYAFDWTRFILLGLALVALIVGLNNLGSGAYISFIVLLLFSIGL
TTLFVKKSKNATKALFSLDVFKNKAFLFSFLPYIMLQFANVGINFLLPNYVQDVFQATSLIGGLILLPGSLLNGFGQPVY
GWMLDNFGGKLPLYMGDILFTLALASLALWGPHMGVLGITIAYLIFAIGRSMAFGNSVAYGLKHINQELQNDANALYNTG
QQVAGAIGTTVLALMMGSVHHPGYTRAQNISAGSGWAFILLVILGLIILGFFHRLVNLDKQIKQTN

Sequences:

>Translated_466_residues
MQESSTKKSNPMVVVAIVALMSFMGVLTETSMNVTFPALMREFKVSLSTVQWVTAGYLLMAALVMLTSAYMKRRFTNRQL
FVAAAILFGLGDVICGAALNFWVLLVGRLIQAGCVGLCTPLMVNIILEVVPQHKLGTYIGLANLIILIAPAMGPTFGGAV
VAFASWRMIFWGTLPIALILMVLGSKKIKQYTPTQKQYAFDWTRFILLGLALVALIVGLNNLGSGAYISFIVLLLFSIGL
TTLFVKKSKNATKALFSLDVFKNKAFLFSFLPYIMLQFANVGINFLLPNYVQDVFQATSLIGGLILLPGSLLNGFGQPVY
GWMLDNFGGKLPLYMGDILFTLALASLALWGPHMGVLGITIAYLIFAIGRSMAFGNSVAYGLKHINQELQNDANALYNTG
QQVAGAIGTTVLALMMGSVHHPGYTRAQNISAGSGWAFILLVILGLIILGFFHRLVNLDKQIKQTN
>Mature_466_residues
MQESSTKKSNPMVVVAIVALMSFMGVLTETSMNVTFPALMREFKVSLSTVQWVTAGYLLMAALVMLTSAYMKRRFTNRQL
FVAAAILFGLGDVICGAALNFWVLLVGRLIQAGCVGLCTPLMVNIILEVVPQHKLGTYIGLANLIILIAPAMGPTFGGAV
VAFASWRMIFWGTLPIALILMVLGSKKIKQYTPTQKQYAFDWTRFILLGLALVALIVGLNNLGSGAYISFIVLLLFSIGL
TTLFVKKSKNATKALFSLDVFKNKAFLFSFLPYIMLQFANVGINFLLPNYVQDVFQATSLIGGLILLPGSLLNGFGQPVY
GWMLDNFGGKLPLYMGDILFTLALASLALWGPHMGVLGITIAYLIFAIGRSMAFGNSVAYGLKHINQELQNDANALYNTG
QQVAGAIGTTVLALMMGSVHHPGYTRAQNISAGSGWAFILLVILGLIILGFFHRLVNLDKQIKQTN

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=412, Percent_Identity=25.9708737864078, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789042, Length=441, Percent_Identity=22.6757369614512, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081983, Length=306, Percent_Identity=22.2222222222222, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788392, Length=426, Percent_Identity=23.7089201877934, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788710, Length=425, Percent_Identity=21.6470588235294, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50606; Mature: 50606

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]