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The map label for this gene is glnH [H]

Identifier: 116492193

GI number: 116492193

Start: 435850

End: 436686

Strand: Direct

Name: glnH [H]

Synonym: PEPE_0390

Alternate gene names: 116492193

Gene position: 435850-436686 (Clockwise)

Preceding gene: 116492192

Following gene: 116492194

Centisome position: 23.79

GC content: 38.47

Gene sequence:

>837_bases
ATGCGAAAAATACTGAAAAGTGTCGCCCTCATTTTCACCCTCATGGCAAGTTTAGTGTTGTTAAGTAGTTGTGGTGCGAA
GGGTGAAGTAAAAAATATAGATGCTTGGGATCAAGCCCAAAAGACGGATTCCATCACTTGGGGTGTTAAAGCCGATACTA
AACTGTTTGGTTTGATGGATGTTAAAGATTCACAAATTAAGGGATTTGATATCGATATTGCCAAAGCGTTAACCAAGCAA
ATGCTCGGAAAAAAGGCCAAAGCCAACTTTATCCAAGTTACGAGTCAAACTCGGATACCTTTACTGAAAAACGGTAATTT
AGATGCAATTATTGCTACGATGACAATCACGAAAGAACGTGAAAAAGTTGTCGATTTTTCAAATTCATACTTTGATGCTG
GTCAATCGCTTTTAGTTAAAAAAGGTAGCTCCATTAAGAGCGTTAAAGATTTGAATAAGCCCGGAACTAAAGTAATCGGG
GTGGTTGGATCTAATTCCGTCGAGAATATTAAAAAGTTTGCTCCTAAGGCGCAGGTAATTCAGCTTCAAGATTATGCTCA
GGCTTTGACCGCTTTAAAATCTGGTCAAGGAGCAGCGTTAACGACTGATAATGGTATTTTATTTGGGATGGCGGTCGACA
ATCCTAATTATCAAGTTGTTGGAAAAGCCTTTACTAAAGAACCTTATGGGATTGCAATTAATAAAGGACAAGGTCAGTTG
AAAAAGCATCTCAACCAAGCCTTGAAAGAATTGGAAGAAAGTGGCGAGTACAACAAACTAGTCGAAAAATGGTTTGGAAA
TATCGCTGGATTTGACTACAAGGAGGTTGAAAGATGA

Upstream 100 bases:

>100_bases
TAGAAGACGATCAAGCTAAGGAATTCTTTGATGGACAACCAAGTAATGAACGAGCACGCCAATTCTTAAGTAAAATTATT
AAACATTAGGAGGGGAGAAT

Downstream 100 bases:

>100_bases
TTAATATAATTGCTAGTCATGGCTCAGAACTTCTAAGTGGACTGGGACAAACCGTCCTTTGTAGTGTGATTGCACTATTC
TTTAGTTTGATCATCGGATC

Product: amino acid ABC transporter periplasmic protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MRKILKSVALIFTLMASLVLLSSCGAKGEVKNIDAWDQAQKTDSITWGVKADTKLFGLMDVKDSQIKGFDIDIAKALTKQ
MLGKKAKANFIQVTSQTRIPLLKNGNLDAIIATMTITKEREKVVDFSNSYFDAGQSLLVKKGSSIKSVKDLNKPGTKVIG
VVGSNSVENIKKFAPKAQVIQLQDYAQALTALKSGQGAALTTDNGILFGMAVDNPNYQVVGKAFTKEPYGIAINKGQGQL
KKHLNQALKELEESGEYNKLVEKWFGNIAGFDYKEVER

Sequences:

>Translated_278_residues
MRKILKSVALIFTLMASLVLLSSCGAKGEVKNIDAWDQAQKTDSITWGVKADTKLFGLMDVKDSQIKGFDIDIAKALTKQ
MLGKKAKANFIQVTSQTRIPLLKNGNLDAIIATMTITKEREKVVDFSNSYFDAGQSLLVKKGSSIKSVKDLNKPGTKVIG
VVGSNSVENIKKFAPKAQVIQLQDYAQALTALKSGQGAALTTDNGILFGMAVDNPNYQVVGKAFTKEPYGIAINKGQGQL
KKHLNQALKELEESGEYNKLVEKWFGNIAGFDYKEVER
>Mature_278_residues
MRKILKSVALIFTLMASLVLLSSCGAKGEVKNIDAWDQAQKTDSITWGVKADTKLFGLMDVKDSQIKGFDIDIAKALTKQ
MLGKKAKANFIQVTSQTRIPLLKNGNLDAIIATMTITKEREKVVDFSNSYFDAGQSLLVKKGSSIKSVKDLNKPGTKVIG
VVGSNSVENIKKFAPKAQVIQLQDYAQALTALKSGQGAALTTDNGILFGMAVDNPNYQVVGKAFTKEPYGIAINKGQGQL
KKHLNQALKELEESGEYNKLVEKWFGNIAGFDYKEVER

Specific function: Part of the ABC transporter complex glnHMPQ involved in glutamine transport [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=203, Percent_Identity=33.9901477832512, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786876, Length=202, Percent_Identity=31.6831683168317, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787088, Length=232, Percent_Identity=30.6034482758621, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787085, Length=232, Percent_Identity=29.7413793103448, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788228, Length=207, Percent_Identity=30.4347826086957, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788648, Length=223, Percent_Identity=26.9058295964126, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788649, Length=209, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30354; Mature: 30354

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKILKSVALIFTLMASLVLLSSCGAKGEVKNIDAWDQAQKTDSITWGVKADTKLFGLMD
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCEEECCCCCCEEEEEEE
VKDSQIKGFDIDIAKALTKQMLGKKAKANFIQVTSQTRIPLLKNGNLDAIIATMTITKER
CCCCCCCCCCHHHHHHHHHHHHCCHHCCCEEEEECCCCCCEEECCCCCEEEEEEEEHHHH
EKVVDFSNSYFDAGQSLLVKKGSSIKSVKDLNKPGTKVIGVVGSNSVENIKKFAPKAQVI
HHHHHCCCCHHHCCCCEEEECCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCHHEE
QLQDYAQALTALKSGQGAALTTDNGILFGMAVDNPNYQVVGKAFTKEPYGIAINKGQGQL
EHHHHHHHHHHHHCCCCCEEEECCCEEEEEEECCCCEEEEEHHHCCCCCEEEEECCHHHH
KKHLNQALKELEESGEYNKLVEKWFGNIAGFDYKEVER
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure
MRKILKSVALIFTLMASLVLLSSCGAKGEVKNIDAWDQAQKTDSITWGVKADTKLFGLMD
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCEEECCCCCCEEEEEEE
VKDSQIKGFDIDIAKALTKQMLGKKAKANFIQVTSQTRIPLLKNGNLDAIIATMTITKER
CCCCCCCCCCHHHHHHHHHHHHCCHHCCCEEEEECCCCCCEEECCCCCEEEEEEEEHHHH
EKVVDFSNSYFDAGQSLLVKKGSSIKSVKDLNKPGTKVIGVVGSNSVENIKKFAPKAQVI
HHHHHCCCCHHHCCCCEEEECCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCHHEE
QLQDYAQALTALKSGQGAALTTDNGILFGMAVDNPNYQVVGKAFTKEPYGIAINKGQGQL
EHHHHHHHHHHHHCCCCCEEEECCCEEEEEEECCCCEEEEEHHHCCCCCEEEEECCHHHH
KKHLNQALKELEESGEYNKLVEKWFGNIAGFDYKEVER
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 9384377 [H]