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The map label for this gene is pyrAA [H]

Identifier: 116492118

GI number: 116492118

Start: 359267

End: 360352

Strand: Direct

Name: pyrAA [H]

Synonym: PEPE_0315

Alternate gene names: 116492118

Gene position: 359267-360352 (Clockwise)

Preceding gene: 116492117

Following gene: 116492119

Centisome position: 19.61

GC content: 40.7

Gene sequence:

>1086_bases
ATGAAACGTTATTTAATTTTGGAAGACGGTACTACATATGAAGGAAAGGCGATTGGAGCCAATCGTGAAAGTATTGGAGA
GCTAGTTTTTAATACTGGGATGTCAGGATATCAAGAATCGATTACCGACCAATCATATAATGGGCAAATTTTAATGTTTA
CCTATCCGTTAATCGGAAATTATGGCGTTAATCGAGATGATTTAGAATCCGTTAAGTCAAGTTGCTTTGGAGTAGTAGTC
CATGAGGTGGCACGTCGAGCAAGTAATTGGCAAATGAATATGAGCTTTGAAGAGTTTTTAGCATATCAAGATTTGCCCGG
TATTAGTGGAGTGGACACGCGAGCAATTACTCGACATATTCGCGAACATGGAGCGATGAAAGCAGCCTTAGTTAATGAAA
TTTCAGACGCTAAGTTTGAAAAATTTCATGCAACTGATTTGATACAAAATCAAATTGAAATTAGCTCAACTCATAGTCCG
TACCCTAATCCAGCCAACGGTCGTAAAGTCGTAGTAATTGATTTTGGACTCAAGTATTCCATCCTACGTGAACTTGCTCG
TCGCGACTGCAACGTAATGGTACTTCCATATAATGCTACGGCAAAAGAAGTTTTAGCTTGCAATCCGGATGGGGTGATGT
TTACTAATGGACCTGGTGATCCGGAATCAATGGATGAAAGTGTTCTAGAAATGATTCGCACAGTGGAGGCTGCGGTTCCA
GTCTTTGGGATTTGTATGGGACACCAACTTTTTGCCCTAGCAAATGGTGCTAAGACAACTAAGATGAAATTTGGACATCG
CGGTTTTAACCATCCAGTTCGCAACTTAGCAACGGGACGAATTGATTTAACTTCCCAAAACCATGGTTATGCGGTCGAAC
GTGCTTCCATTGCAAACACTGACCTTGAAATTACCCACGAAGAAATTAATGACGAAACCGTTGAAGGACTCAGACATAAA
CAATATAAGGCTTTTTCCGTTCAATTCCATCCAGATGCTGCACCGGGTCCTCATGATGCGGACTACCTTTTTGATGATTT
TATGGAAATGATTGATGAAGCTAAACAAACCCAGGAGGACAAGTAA

Upstream 100 bases:

>100_bases
GGGAATCAATTCTCCGTTTATTGGTCAAAAGGTTTATGGACAGACACAACTGACCATGGTTGCTGGAAAAATTGTATATC
AACGGGAAGGGTAATTTAAA

Downstream 100 bases:

>100_bases
ACATGCCTAAGAGAGAGGATATTAATAAAATTTTAGTACTCGGTTCTGGACCCATCATTATCGGTCAAGCGGCTGAGTTC
GATTATTCTGGAACTCAAGC

Product: carbamoyl-phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain; CPS-P [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKRYLILEDGTTYEGKAIGANRESIGELVFNTGMSGYQESITDQSYNGQILMFTYPLIGNYGVNRDDLESVKSSCFGVVV
HEVARRASNWQMNMSFEEFLAYQDLPGISGVDTRAITRHIREHGAMKAALVNEISDAKFEKFHATDLIQNQIEISSTHSP
YPNPANGRKVVVIDFGLKYSILRELARRDCNVMVLPYNATAKEVLACNPDGVMFTNGPGDPESMDESVLEMIRTVEAAVP
VFGICMGHQLFALANGAKTTKMKFGHRGFNHPVRNLATGRIDLTSQNHGYAVERASIANTDLEITHEEINDETVEGLRHK
QYKAFSVQFHPDAAPGPHDADYLFDDFMEMIDEAKQTQEDK

Sequences:

>Translated_361_residues
MKRYLILEDGTTYEGKAIGANRESIGELVFNTGMSGYQESITDQSYNGQILMFTYPLIGNYGVNRDDLESVKSSCFGVVV
HEVARRASNWQMNMSFEEFLAYQDLPGISGVDTRAITRHIREHGAMKAALVNEISDAKFEKFHATDLIQNQIEISSTHSP
YPNPANGRKVVVIDFGLKYSILRELARRDCNVMVLPYNATAKEVLACNPDGVMFTNGPGDPESMDESVLEMIRTVEAAVP
VFGICMGHQLFALANGAKTTKMKFGHRGFNHPVRNLATGRIDLTSQNHGYAVERASIANTDLEITHEEINDETVEGLRHK
QYKAFSVQFHPDAAPGPHDADYLFDDFMEMIDEAKQTQEDK
>Mature_361_residues
MKRYLILEDGTTYEGKAIGANRESIGELVFNTGMSGYQESITDQSYNGQILMFTYPLIGNYGVNRDDLESVKSSCFGVVV
HEVARRASNWQMNMSFEEFLAYQDLPGISGVDTRAITRHIREHGAMKAALVNEISDAKFEKFHATDLIQNQIEISSTHSP
YPNPANGRKVVVIDFGLKYSILRELARRDCNVMVLPYNATAKEVLACNPDGVMFTNGPGDPESMDESVLEMIRTVEAAVP
VFGICMGHQLFALANGAKTTKMKFGHRGFNHPVRNLATGRIDLTSQNHGYAVERASIANTDLEITHEEINDETVEGLRHK
QYKAFSVQFHPDAAPGPHDADYLFDDFMEMIDEAKQTQEDK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=366, Percent_Identity=40.1639344262295, Blast_Score=259, Evalue=2e-69,
Organism=Homo sapiens, GI169790915, Length=367, Percent_Identity=34.8773841961853, Blast_Score=221, Evalue=7e-58,
Organism=Homo sapiens, GI21361331, Length=367, Percent_Identity=34.8773841961853, Blast_Score=221, Evalue=8e-58,
Organism=Escherichia coli, GI1786215, Length=379, Percent_Identity=40.8970976253298, Blast_Score=293, Evalue=9e-81,
Organism=Caenorhabditis elegans, GI193204318, Length=369, Percent_Identity=39.8373983739837, Blast_Score=259, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6322331, Length=396, Percent_Identity=37.8787878787879, Blast_Score=253, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6324878, Length=393, Percent_Identity=37.1501272264631, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6322638, Length=182, Percent_Identity=32.4175824175824, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI45555749, Length=382, Percent_Identity=40.5759162303665, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24642586, Length=382, Percent_Identity=40.5759162303665, Blast_Score=253, Evalue=1e-67,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40314; Mature: 40314

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRYLILEDGTTYEGKAIGANRESIGELVFNTGMSGYQESITDQSYNGQILMFTYPLIGN
CCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEEECCCCC
YGVNRDDLESVKSSCFGVVVHEVARRASNWQMNMSFEEFLAYQDLPGISGVDTRAITRHI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHCCCCCCCCCHHHHHHHH
REHGAMKAALVNEISDAKFEKFHATDLIQNQIEISSTHSPYPNPANGRKVVVIDFGLKYS
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEEECCCCHH
ILRELARRDCNVMVLPYNATAKEVLACNPDGVMFTNGPGDPESMDESVLEMIRTVEAAVP
HHHHHHHCCCCEEEEECCCCCCHHEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VFGICMGHQLFALANGAKTTKMKFGHRGFNHPVRNLATGRIDLTSQNHGYAVERASIANT
HHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHCCCEEEEECCCCCEEEEECHHCCC
DLEITHEEINDETVEGLRHKQYKAFSVQFHPDAAPGPHDADYLFDDFMEMIDEAKQTQED
CCEEEHHHCCHHHHHHHHHCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
K
C
>Mature Secondary Structure
MKRYLILEDGTTYEGKAIGANRESIGELVFNTGMSGYQESITDQSYNGQILMFTYPLIGN
CCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEEECCCCC
YGVNRDDLESVKSSCFGVVVHEVARRASNWQMNMSFEEFLAYQDLPGISGVDTRAITRHI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHCCCCCCCCCHHHHHHHH
REHGAMKAALVNEISDAKFEKFHATDLIQNQIEISSTHSPYPNPANGRKVVVIDFGLKYS
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEEECCCCHH
ILRELARRDCNVMVLPYNATAKEVLACNPDGVMFTNGPGDPESMDESVLEMIRTVEAAVP
HHHHHHHCCCCEEEEECCCCCCHHEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VFGICMGHQLFALANGAKTTKMKFGHRGFNHPVRNLATGRIDLTSQNHGYAVERASIANT
HHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHCCCEEEEECCCCCEEEEECHHCCC
DLEITHEEINDETVEGLRHKQYKAFSVQFHPDAAPGPHDADYLFDDFMEMIDEAKQTQED
CCEEEHHHCCHHHHHHHHHCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8982065; 12566566; 10852872 [H]