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The map label for this gene is aroA

Identifier: 116328725

GI number: 116328725

Start: 2458305

End: 2459627

Strand: Reverse

Name: aroA

Synonym: LBL_2099

Alternate gene names: 116328725

Gene position: 2459627-2458305 (Counterclockwise)

Preceding gene: 116328726

Following gene: 116328724

Centisome position: 68.05

GC content: 44.6

Gene sequence:

>1323_bases
ATGATTCTAAAAAACGTTAAACTCAAATCCGGAGAAATTACGGTTCCCGGAGATAAATCCCTTTCTCATCGCTCCGTTCT
GTTTGCCGCTCTTTGCAAGGGGAAATCTAAAGTGACCGGATTTTTGGAAGCGGAAGATCCTTTGAACACGATGTCTGCGT
TCACCAAACTCGGATTGAAGGTCCGAAAAGTCAAACCGGGAGAATACGAGTTTGAAAGTCCGGGTAAGAGAGGGTTTATT
TCGCCTAACGTGGATCTTGATTTTGGAAACGCCGGAACGGGGATTCGATTGTCTGCGGGACTCCTTTGCGGTTTACCCGG
AGTAAACGCGGTCCTAACGGGAGACGGTTCCCTAAAAAAACGTCCCATGGGTAGAATCATTAAACCTTTGACTGCCATGG
GAGCTTCCATTCTCGGTCTGGGGGAAAAAGAAACCGCTCCTTTAAAAGTGGAAGGAAAAAAACTAAAAAGCTTTCGTTAT
GAAAGTCCGATCGCGAGCGCACAGATTAAGTCCTGCTTGATGCTTGCGGCAATTGCATCCGAAACGGATTTGGAATATTC
GGAAAATATACTATCTCGGGACCACACGGAGAATATGTTCCGATTTTTAGGCAATAAGATCGAGCAAATTTCCCCGTTTC
ATTTTAAGATCGAACCTCCATATGTTCTGAATGGAGGGGAATTTAAGGTGCCGGGTGATATTTCTTCCGCAGCGTTTTTT
TTGGTTCTCGGGGTTTTGGCCAAAGAAGGAAATTTACTGGTTAGAAACATAGGGCTCAATCCGGCCCGAATCGGAATTTT
GAAAGCTCTCGAACTGATGGGCGCCAAAATCGAAATCCGGAATCAGAGGATGGAATGTGGAGAACCGGTCGGGGATCTAA
AGACTTACCCTTCTATATTAAATAAAACGAATATTCCGAAATCTTTGATTCCTTCTATCATCGACGAAATCCCGATCCTT
TCTGTCGCGGGACTTTTCGCGAAAGGCGGTTTTGAAATCCGTCATGCGGAGGAACTACGCGCTAAGGAATCGGACCGGAT
CCATACGATGGTTTCTAACTTTCGGGCGCTTGGGATCGAAGTAGAGGAATATCCGGACGGATATGCGTTTGACGGAACCT
CGCCTCAATCTTTGGAGATTTGGAAATTTCTCGCCTCCGGAAAAAAAATCTCCATTCTTTCGCATATGGATCATCGAATC
ACAATGAGCTTTTTGATTTTCAAAACACTTTCCGGATTTAATCTCCACATAGACGAAACTTCCTGGATCGAAACTTCTTT
TCCGGGTTTTGAAAAATTACTCGAGAGTTGTTTGGATGAATGA

Upstream 100 bases:

>100_bases
TTAGAAAAATTGAATCCAAAAAACGTTATAGATCCGAAGGAATGGGAACGGTTTATGGAAACTTCCCGTAGATCCAGAGA
TTATATTTTGAAGAGCCAGG

Downstream 100 bases:

>100_bases
AAATGTAATCGCTTTGGATGGTCCGGCCGGTTCGGGCAAAAGTACGGTGGCAAGGCAAATCGCCGAAAGAATCGGATTCA
ATTATTTGGATACCGGCGCG

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MILKNVKLKSGEITVPGDKSLSHRSVLFAALCKGKSKVTGFLEAEDPLNTMSAFTKLGLKVRKVKPGEYEFESPGKRGFI
SPNVDLDFGNAGTGIRLSAGLLCGLPGVNAVLTGDGSLKKRPMGRIIKPLTAMGASILGLGEKETAPLKVEGKKLKSFRY
ESPIASAQIKSCLMLAAIASETDLEYSENILSRDHTENMFRFLGNKIEQISPFHFKIEPPYVLNGGEFKVPGDISSAAFF
LVLGVLAKEGNLLVRNIGLNPARIGILKALELMGAKIEIRNQRMECGEPVGDLKTYPSILNKTNIPKSLIPSIIDEIPIL
SVAGLFAKGGFEIRHAEELRAKESDRIHTMVSNFRALGIEVEEYPDGYAFDGTSPQSLEIWKFLASGKKISILSHMDHRI
TMSFLIFKTLSGFNLHIDETSWIETSFPGFEKLLESCLDE

Sequences:

>Translated_440_residues
MILKNVKLKSGEITVPGDKSLSHRSVLFAALCKGKSKVTGFLEAEDPLNTMSAFTKLGLKVRKVKPGEYEFESPGKRGFI
SPNVDLDFGNAGTGIRLSAGLLCGLPGVNAVLTGDGSLKKRPMGRIIKPLTAMGASILGLGEKETAPLKVEGKKLKSFRY
ESPIASAQIKSCLMLAAIASETDLEYSENILSRDHTENMFRFLGNKIEQISPFHFKIEPPYVLNGGEFKVPGDISSAAFF
LVLGVLAKEGNLLVRNIGLNPARIGILKALELMGAKIEIRNQRMECGEPVGDLKTYPSILNKTNIPKSLIPSIIDEIPIL
SVAGLFAKGGFEIRHAEELRAKESDRIHTMVSNFRALGIEVEEYPDGYAFDGTSPQSLEIWKFLASGKKISILSHMDHRI
TMSFLIFKTLSGFNLHIDETSWIETSFPGFEKLLESCLDE
>Mature_440_residues
MILKNVKLKSGEITVPGDKSLSHRSVLFAALCKGKSKVTGFLEAEDPLNTMSAFTKLGLKVRKVKPGEYEFESPGKRGFI
SPNVDLDFGNAGTGIRLSAGLLCGLPGVNAVLTGDGSLKKRPMGRIIKPLTAMGASILGLGEKETAPLKVEGKKLKSFRY
ESPIASAQIKSCLMLAAIASETDLEYSENILSRDHTENMFRFLGNKIEQISPFHFKIEPPYVLNGGEFKVPGDISSAAFF
LVLGVLAKEGNLLVRNIGLNPARIGILKALELMGAKIEIRNQRMECGEPVGDLKTYPSILNKTNIPKSLIPSIIDEIPIL
SVAGLFAKGGFEIRHAEELRAKESDRIHTMVSNFRALGIEVEEYPDGYAFDGTSPQSLEIWKFLASGKKISILSHMDHRI
TMSFLIFKTLSGFNLHIDETSWIETSFPGFEKLLESCLDE

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=452, Percent_Identity=30.0884955752212, Blast_Score=134, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6320332, Length=454, Percent_Identity=25.9911894273128, Blast_Score=97, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_LEPBJ (Q04U42)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_800336.1
- ProteinModelPortal:   Q04U42
- SMR:   Q04U42
- STRING:   Q04U42
- GeneID:   4409830
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_0934
- eggNOG:   COG0128
- HOGENOM:   HBG646626
- OMA:   LSRNHTE
- ProtClustDB:   PRK02427
- BioCyc:   LBOR355277:LBJ_0934-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 48177; Mature: 48177

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILKNVKLKSGEITVPGDKSLSHRSVLFAALCKGKSKVTGFLEAEDPLNTMSAFTKLGLK
CCCCCEEECCCEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCE
VRKVKPGEYEFESPGKRGFISPNVDLDFGNAGTGIRLSAGLLCGLPGVNAVLTGDGSLKK
EEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEECCEEECCCCCCEEEECCCCCCC
RPMGRIIKPLTAMGASILGLGEKETAPLKVEGKKLKSFRYESPIASAQIKSCLMLAAIAS
CCHHHHHHHHHHHCHHHCCCCCCCCCCEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHC
ETDLEYSENILSRDHTENMFRFLGNKIEQISPFHFKIEPPYVLNGGEFKVPGDISSAAFF
CCCCHHHHHHHCCCHHHHHHHHHCCHHHHCCCCEEEECCCEEECCCCEECCCCCHHHHHH
LVLGVLAKEGNLLVRNIGLNPARIGILKALELMGAKIEIRNQRMECGEPVGDLKTYPSIL
HHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEECCHHHCCCCHHHHHHHHHHH
NKTNIPKSLIPSIIDEIPILSVAGLFAKGGFEIRHAEELRAKESDRIHTMVSNFRALGIE
CCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEECHHHHHHCCCHHHHHHHHHHHHCCEE
VEEYPDGYAFDGTSPQSLEIWKFLASGKKISILSHMDHRITMSFLIFKTLSGFNLHIDET
HHHCCCCEEECCCCCCHHHHHHHHHCCCEEEEEHHCCHHHHHHHHHHHHHCCCEEEECCC
SWIETSFPGFEKLLESCLDE
CCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MILKNVKLKSGEITVPGDKSLSHRSVLFAALCKGKSKVTGFLEAEDPLNTMSAFTKLGLK
CCCCCEEECCCEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCE
VRKVKPGEYEFESPGKRGFISPNVDLDFGNAGTGIRLSAGLLCGLPGVNAVLTGDGSLKK
EEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEECCEEECCCCCCEEEECCCCCCC
RPMGRIIKPLTAMGASILGLGEKETAPLKVEGKKLKSFRYESPIASAQIKSCLMLAAIAS
CCHHHHHHHHHHHCHHHCCCCCCCCCCEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHC
ETDLEYSENILSRDHTENMFRFLGNKIEQISPFHFKIEPPYVLNGGEFKVPGDISSAAFF
CCCCHHHHHHHCCCHHHHHHHHHCCHHHHCCCCEEEECCCEEECCCCEECCCCCHHHHHH
LVLGVLAKEGNLLVRNIGLNPARIGILKALELMGAKIEIRNQRMECGEPVGDLKTYPSIL
HHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEECCHHHCCCCHHHHHHHHHHH
NKTNIPKSLIPSIIDEIPILSVAGLFAKGGFEIRHAEELRAKESDRIHTMVSNFRALGIE
CCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEECHHHHHHCCCHHHHHHHHHHHHCCEE
VEEYPDGYAFDGTSPQSLEIWKFLASGKKISILSHMDHRITMSFLIFKTLSGFNLHIDET
HHHCCCCEEECCCCCCHHHHHHHHHCCCEEEEEHHCCHHHHHHHHHHHHHCCCEEEECCC
SWIETSFPGFEKLLESCLDE
CCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA