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The map label for this gene is tetA [H]

Identifier: 116328455

GI number: 116328455

Start: 2091731

End: 2093026

Strand: Direct

Name: tetA [H]

Synonym: LBL_1798

Alternate gene names: 116328455

Gene position: 2091731-2093026 (Clockwise)

Preceding gene: 116328454

Following gene: 116328461

Centisome position: 57.87

GC content: 44.83

Gene sequence:

>1296_bases
ATGAATAAAATTTCCTTCGTCCTTTTCTTTACCGTATTCATCGATATGATGGGGTTCTCCGTGATCTTTCCCATCTTTCC
CGAAACGCTCAAAGCCTTTCTCGCTAAACCCGGTGATCCGGTCTTAGATAAATTCACTGATCTTACAAGAATTCTTGTGG
AAGTTTCCGGTGGAGATTGGGCTTTATTCGTGGCCCTCTTCGGTGGAATTGTCGCCAGTCTTTATTCCCTTCTACAGTTT
GCATTCGCTCCGATTTGGGGAAGAATCTCCGACCGTGTCGGAAGAAAACCCGTCCTTGTGTTCACAAGTCTAGGAAGTTT
TTTCGGATACGCGGTTTGGTTCTTTTCGGGAAGTTTTTCCCTGTTCGTATTCTCCAGAGTCATCACAGGAATGATGGGAG
GAAATATCTCCGTCGCGTCCGCAGCGATGGCCGACATCACGAGTGAAAAGGATCGCGCCAAAGGAATGGGTTTGATCGGA
GCCGGAGTCGGACTCGGCTTTATCGCAGGCCCGCCAACAGGAGGATTGTTTTCAAAAATCGATCTAAGCTTTTTGGAACT
CGCGTTTCCTGATATTCCGTTCACCGTGTTTCCGGCTTCCGCGCTTGTAGCGGCGGCGATCGCGCTCCTCAATCTTTTGA
TGATCGTTTTCTGGTTTAAGGAAACCTTAAACGTAAACCAGGAAGTCCAGGAAAAGAAAAAGATCCATCCGATCATCGGA
GTCTTTGCGTCGAACAACAGAGAAGTGGTTTCGTATTCCCTTCTTTATTTCGTTTTCGTGTTCGCGTTTTCCGGTTTCGA
ATTTTCAATCAACTTTTACTTAAGTCAATTTTTGAATTACAGTCCGATCTCGATCGGCTTGACTTTCGTTTATATCGGGA
TGATTATCGTTTTGATTCAAGGCGGAGTGTTTCGAAGACTTTCCGGCAAGGTAAAGGAAACAAAACTCGTACGAGCGGGC
GCCCTCTTCTTGATCGTCGGATTTTCTTTTCTCTATTTTGTTTCAAATTCCTATCAGCTTTTTATATCGCTTACCTTTTT
GGCTTCGGGAAGCGCGCTGCTTCATCCTTCCCTTTCCACATTAGTTTCTCTTGTCTCCAGTCAGGAAGAACAAGGAACAA
ATCTCGGGATGTTTCGAAGTCTCGCTTCCTTGGGAAGAGGACTTGCTCCATTTGCATTTTGTTTAATTTATTTCAGCAGA
GGTCCCTCCATCTCGTTTCTAACTTCGGGCTTTATCTGTTTTCTATTTCTACTTTTTATCTGGAACCTGAAACAGCCAAG
TCACAGTCACCTATAA

Upstream 100 bases:

>100_bases
GCGTCTCTTAAGTAAGAAACAATAGCCCCTCTTTTTTCGAAAATGTTAAGACAAGGTAAGTGGCTTAGAAGATCGTTGTC
TTCGAGCCATTTTACCTCTT

Downstream 100 bases:

>100_bases
ATTAACGTGAGTTCGGCGTAAGAAAGTTTGGGCGAATAAGAAACTAAAGTGTTACGACTCCCTATGAGTCGTCGCAGCTC
GCGAATTTCATAGATAAAAT

Product: major facilitator superfamily permease

Products: NA

Alternate protein names: TetA(B); Metal-tetracycline/H(+) antiporter [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MNKISFVLFFTVFIDMMGFSVIFPIFPETLKAFLAKPGDPVLDKFTDLTRILVEVSGGDWALFVALFGGIVASLYSLLQF
AFAPIWGRISDRVGRKPVLVFTSLGSFFGYAVWFFSGSFSLFVFSRVITGMMGGNISVASAAMADITSEKDRAKGMGLIG
AGVGLGFIAGPPTGGLFSKIDLSFLELAFPDIPFTVFPASALVAAAIALLNLLMIVFWFKETLNVNQEVQEKKKIHPIIG
VFASNNREVVSYSLLYFVFVFAFSGFEFSINFYLSQFLNYSPISIGLTFVYIGMIIVLIQGGVFRRLSGKVKETKLVRAG
ALFLIVGFSFLYFVSNSYQLFISLTFLASGSALLHPSLSTLVSLVSSQEEQGTNLGMFRSLASLGRGLAPFAFCLIYFSR
GPSISFLTSGFICFLFLLFIWNLKQPSHSHL

Sequences:

>Translated_431_residues
MNKISFVLFFTVFIDMMGFSVIFPIFPETLKAFLAKPGDPVLDKFTDLTRILVEVSGGDWALFVALFGGIVASLYSLLQF
AFAPIWGRISDRVGRKPVLVFTSLGSFFGYAVWFFSGSFSLFVFSRVITGMMGGNISVASAAMADITSEKDRAKGMGLIG
AGVGLGFIAGPPTGGLFSKIDLSFLELAFPDIPFTVFPASALVAAAIALLNLLMIVFWFKETLNVNQEVQEKKKIHPIIG
VFASNNREVVSYSLLYFVFVFAFSGFEFSINFYLSQFLNYSPISIGLTFVYIGMIIVLIQGGVFRRLSGKVKETKLVRAG
ALFLIVGFSFLYFVSNSYQLFISLTFLASGSALLHPSLSTLVSLVSSQEEQGTNLGMFRSLASLGRGLAPFAFCLIYFSR
GPSISFLTSGFICFLFLLFIWNLKQPSHSHL
>Mature_431_residues
MNKISFVLFFTVFIDMMGFSVIFPIFPETLKAFLAKPGDPVLDKFTDLTRILVEVSGGDWALFVALFGGIVASLYSLLQF
AFAPIWGRISDRVGRKPVLVFTSLGSFFGYAVWFFSGSFSLFVFSRVITGMMGGNISVASAAMADITSEKDRAKGMGLIG
AGVGLGFIAGPPTGGLFSKIDLSFLELAFPDIPFTVFPASALVAAAIALLNLLMIVFWFKETLNVNQEVQEKKKIHPIIG
VFASNNREVVSYSLLYFVFVFAFSGFEFSINFYLSQFLNYSPISIGLTFVYIGMIIVLIQGGVFRRLSGKVKETKLVRAG
ALFLIVGFSFLYFVSNSYQLFISLTFLASGSALLHPSLSTLVSLVSSQEEQGTNLGMFRSLASLGRGLAPFAFCLIYFSR
GPSISFLTSGFICFLFLLFIWNLKQPSHSHL

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Homo sapiens, GI225703104, Length=368, Percent_Identity=29.6195652173913, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI225703102, Length=368, Percent_Identity=29.6195652173913, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI39753965, Length=351, Percent_Identity=24.5014245014245, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17551570, Length=394, Percent_Identity=30.2030456852792, Blast_Score=151, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI71996331, Length=351, Percent_Identity=24.7863247863248, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17738085, Length=426, Percent_Identity=30.5164319248826, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI221513297, Length=344, Percent_Identity=25.2906976744186, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47290; Mature: 47290

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCCCCCC
>Mature Secondary Structure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HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6319234; 6298728; 1848846; 2168416; 8995300 [H]